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  4. Human dynorphin,Dyn ELISA Kit

Human dynorphin,Dyn ELISA Kit

  • 中文名稱：
    人強啡肽(Dyn)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E09128h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人強啡肽(Dyn)酶聯(lián)免疫試劑盒（CSB-E09128h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Dyn含量。人強啡肽（Dyn）是一種具有阿片樣活性的內(nèi)源性肽，主要作用于κ-阿片受體，具有鎮(zhèn)痛、減輕腦水腫、改善缺血后腦記憶障礙等腦保護作用。其作用機制主要通過阿片樣受體途徑和非阿片樣受體途徑實現(xiàn)，如κ受體介導的鎮(zhèn)痛和NMDA受體途徑的腦保護作用。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，本試劑盒檢測范圍為3.12 pg/mL至200 pg/mL，可精準識別低豐度靶標，適用于神經(jīng)生物學、疼痛機制研究或精神疾病動物模型中的動態(tài)監(jiān)測。操作簡便，僅需100 μL樣本即可完成檢測，配套提供標準品、稀釋液及終止液等完整組分，適用于實驗室規(guī)模的高通量篩查或靶向性研究，為探索Dyn在神經(jīng)信號傳導、藥物干預效果評估等科研領域提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    N/A
  • 縮寫：
    Dyn
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    3.12 pg/mL-200 pg/mL
  • 靈敏度：
    0.78 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human Dyn in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %80-90
    1:2Average %95
    Range %90-100
    1:4Average %93
    Range %90-96
    1:8Average %92
    Range %87-96
  • 回收率：
    The recovery of human Dyn spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9284-99
    EDTA plasma (n=4)10097-103
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.537 2.576 2.557 2.451
    1002.141 2.164 2.153 2.047
    501.778 1.791 1.785 1.679
    251.405 1.455 1.430 1.324
    12.51.012 1.053 1.033 0.927
    6.250.604 0.624 0.614 0.508
    3.120.348 0.357 0.353 0.247
    00.105 0.107 0.106 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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