Human fetal hemoglobin,HBF ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人胎兒血紅蛋白(HBF)酶聯(lián)免疫試劑盒（CSB-E09598h）為競爭法ELISA試劑盒，定量檢測血清、血漿、RBC裂解物樣本中的HBF含量。HBF（胎兒血紅蛋白）具有重要背景。它在胎兒發(fā)育階段是主要血紅蛋白，出生后逐漸被成人血紅蛋白替代。研究發(fā)現(xiàn)，提升 HBF 水平可改善某些血紅蛋白病。其研究機制聚焦于調(diào)控 HBF 表達的基因與信號通路，以探索治療相關疾病的新策略。試劑盒檢測范圍為125 ng/mL-2000 ng/mL，該產(chǎn)品適用于科研領域對胎兒血紅蛋白表達調(diào)控機制的研究，如遺傳性血紅蛋白病的病理機制探索、造血干細胞分化過程中血紅蛋白轉換的分子研究，以及藥物開發(fā)中對胎兒血紅蛋白誘導療法的效果評估，為血液學基礎研究和疾病模型構建提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

HBF
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, lysate for RBC
檢測范圍：

125 ng/mL-2000 ng/mL
靈敏度：

62.5 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human HBF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	95
	Range %	91-99
1:40	Average %	103
	Range %	99-107
1:80	Average %	98
	Range %	93-103
1:160	Average %	92
	Range %	87-97

回收率：

The recovery of human HBF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-104
EDTA plasma (n=4)	89	84-94

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.