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  4. Human glucocorticoid receptor-β,GR-β ELISA Kit

Human glucocorticoid receptor-β,GR-β ELISA Kit

  • 中文名稱：
    人糖皮質(zhì)激素受體β(GR-β)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08889h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人糖皮質(zhì)激素受體β(GR-β)酶聯(lián)免疫試劑盒（CSB-E08889h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的GR-β含量。GR-β即糖皮質(zhì)激素受體β，是核受體超家族成員。它能拮抗GR-α轉(zhuǎn)錄活性，影響糖皮質(zhì)激素效應(yīng)。研究發(fā)現(xiàn)其在哮喘等炎癥性疾病中異常表達(dá)，深入探究其作用機(jī)制，可為相關(guān)疾病的治療提供新的靶點(diǎn)與思路。試劑盒檢測范圍為39 pg/mL-2500 pg/mL，適用于科研領(lǐng)域?qū)R-β表達(dá)水平的精準(zhǔn)分析；可為體外研究GR-β在疾病模型中的表達(dá)調(diào)控、藥物干預(yù)效果評估以及信號通路機(jī)制探索提供可靠工具；實(shí)驗(yàn)樣本兼容性廣泛，支持從基礎(chǔ)細(xì)胞實(shí)驗(yàn)到動物組織研究的多樣化需求，操作流程標(biāo)準(zhǔn)化，適用于實(shí)驗(yàn)室常規(guī)蛋白定量分析本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    lucocorticoid receptor-β,GR-β|lucocorticoid receptor-beta,GR-beta
  • 縮寫：
    GR-β
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    39 pg/mL-2500 pg/mL
  • 靈敏度：
    9.75 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Epigenetics and Nuclear Signaling
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays):CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human GR-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 103    
    Range % 99-108    
    1:2 Average % 98    
    Range % 94-102    
    1:4 Average % 91    
    Range % 88-95    
    1:8 Average % 89    
    Range % 85-96    
  • 回收率：
    The recovery of human GR-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 90-100    
    EDTA plasma (n=4) 87 83-95    
                   
                   
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    2500 2.441 2.382 2.412 2.301    
    1250 1.692 1.725 1.709 1.598    
    625 1.021 1.103 1.062 0.951    
    312.5 0.563 0.539 0.551 0.440    
    156 0.327 0.314 0.321 0.210    
    78 0.245 0.258 0.252 0.141    
    39 0.185 0.191 0.188 0.077    
    0 0.108 0.113 0.111 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

相關(guān)問答

■ 常見問題解答
Q:

Please provide the following information for CSB-E08889h
1.What is the standard? (Recombinant full-length protein? Partial protein
(range?)?, peptide (sequence range?), native protein?)
2. If recombinant protein, then what is the host?
3. Is the capture antibody a monoclonal or polyclonal?
4. What is the host of the capture antibody?
5. Is the detection antibody a monoclonal or polyclonal?
6. What is the host of the detection antibody?

A:
Thanks for your inquiry.
CSB-E08889h
Capture antibody is mouse monoclonal antibody.
Detection antibody is goat polyclonal antibody.
Standard is CHO cell-derived recombinant protein.