Human growth differentiation factor 15(GDF15)ELISA Kit
產(chǎn)品詳情
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產(chǎn)品描述:
人生長(zhǎng)分化因子15(GDF15)酶聯(lián)免疫試劑盒(CSB-E12009h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿、尿液樣本中的GDF15含量。GDF15是一種生長(zhǎng)分化因子。其它在多種生理病理過(guò)程中發(fā)揮作用。研究機(jī)制上,GDF15可通過(guò)結(jié)合特定受體調(diào)控細(xì)胞生長(zhǎng)、分化等活動(dòng),與代謝、腫瘤、心血管疾病等的發(fā)生發(fā)展存在關(guān)聯(lián),在疾病診斷和治療上有潛在價(jià)值。試劑盒檢測(cè)范圍為7.8 pg/mL-500 pg/mL,靈敏度為1.95 pg/mL。該產(chǎn)品適用于基礎(chǔ)科研中探究GDF15在代謝調(diào)控、腫瘤進(jìn)展或慢性炎癥等領(lǐng)域的分子機(jī)制,也可用于體外模型或動(dòng)物實(shí)驗(yàn)中生物標(biāo)志物的定量分析,為疾病相關(guān)分子通路研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
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別名:
GDF 15 ELISA Kit; GDF-15 ELISA Kit; Gdf15 ELISA Kit; GDF15_HUMAN ELISA Kit; Growth differentiation factor 15 ELISA Kit; Growth/differentiation factor 15 ELISA Kit; Macrophage inhibitory cytokine 1 ELISA Kit; MIC 1 ELISA Kit; Mic-1 ELISA Kit; MIC1 ELISA Kit; NAG 1 ELISA Kit; NAG-1 ELISA Kit; NAG1 ELISA Kit; NRG 1 ELISA Kit; NRG-1 ELISA Kit; NRG1 ELISA Kit; NSAID (nonsteroidal anti inflammatory drug) activated protein 1 ELISA Kit; NSAID ELISA Kit; NSAID regulated protein 1 ELISA Kit; NSAID-activated gene 1 protein ELISA Kit; NSAID-regulated gene 1 protein ELISA Kit; PDF ELISA Kit; PLAB ELISA Kit; Placental bone morphogenetic protein ELISA Kit; Placental bone morphogenic protein ELISA Kit; Placental TGF beta ELISA Kit; Placental TGF-beta ELISA Kit; Prostate differentiation factor ELISA Kit; PTGF beta ELISA Kit; PTGFB ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:
Homo sapiens (Human)
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樣本類型:
serum, plasma, tissue homogenates, urine
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檢測(cè)范圍:
7.8 pg/mL-500 pg/mL
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靈敏度:
1.95 pg/mL
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反應(yīng)時(shí)間:
1-5h
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樣本體積:
50-100ul
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檢測(cè)波長(zhǎng):
450 nm
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研究領(lǐng)域:
Signal Transduction
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測(cè)定原理:
quantitative
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測(cè)定方法:
Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% |
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Three samples of known concentration were tested twenty times on one plate to assess. |
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Inter-assay Precision (Precision between assays): CV%<10% |
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Three samples of known concentration were tested in twenty assays to assess. |
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human GDF15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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1:1 |
Average % |
89 |
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Range % |
80-91 |
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1:2 |
Average % |
98 |
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Range % |
91-104 |
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1:4 |
Average % |
97 |
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Range % |
90-105 |
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1:8 |
Average % |
95 |
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Range % |
91-100 |
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回收率:
The recovery of human GDF15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
Sample Type |
Average % Recovery |
Range |
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Serum (n=5) |
92 |
86-99 |
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EDTA plasma (n=4) |
97 |
91-105 |
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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500 |
1.756 |
1.737 |
1.747 |
1.601 |
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250 |
1.134 |
1.142 |
1.138 |
0.992 |
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125 |
0.702 |
0.719 |
0.711 |
0.565 |
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62.5 |
0.465 |
0.450 |
0.458 |
0.312 |
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31.2 |
0.337 |
0.349 |
0.343 |
0.197 |
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15.6 |
0.266 |
0.272 |
0.269 |
0.123 |
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7.8 |
0.192 |
0.203 |
0.198 |
0.052 |
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0 |
0.145 |
0.147 |
0.146 |
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本試劑盒所含材料:
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human GDF15 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled GDF15 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料:
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數(shù)據(jù)處理:
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貨期:
3-5 working days
引用文獻(xiàn)
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Impact of three types of resistance, aerobic, and combined exercises on serum levels of cardiac biomarkers in active older adults
H Norinejad, A Salehi,Sustainability and Sports Science Journal,2025
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Correlation Between Growth Differentiation Factor-15 and Peripheral Neuropathy in Patients with Type 2 Diabetes Mellitus
Y Li, Y Wang, Y Cao, X Zhang, W Dai,Diabetes, Metabolic Syndrome and Obesity,2024
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The microbial metabolite agmatine acts as an FXR agonist to promote polycystic ovary syndrome in female mice
C Yun,Nature metabolism,2024
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Potential Association of Gut Microbial Metabolism and Circulating mRNA Based on Multiomics Sequencing Analysis in Fetal Growth Restriction
H Tang,Mediators of inflammation,2024
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Association of Growth and Differentiation Factor 15 in Rheumatoid Arthritis
YW He,International journal of clinical practice,2022
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Association between Growth Differentiation Factor-15 and Risk of Cardiovascular Diseases in Patients with Adult Growth Hormone Deficiency
X Wu,International Journal of Endocrinology,2021
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The Association Among Serum Growth Differentiation Factor 15 Level and Suicidal Ideation is Dependent on Testosterone Deficiency in Depressive Patients
Rui Peng,Journal of Inflammation Research,2021
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The association between serum growth differentiation factor-15 and 3-month depression after acute ischemic stroke
Xiurong Lu, et al,Journal of Affective Disorders,2019
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Association of serum growth differentiation factor 15 and hepatocellular carcinoma in Egyptian patients
Mohamed-Naguib Wifi,Egypt J Intern Med,2019
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Relation of growth differentiation factor-15 with CHA2DS2-VASC score in Chinese patients with non-valvular atrial fibrillation
Liu T.et al, International journal of cardiology,2015
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Regulation of Hepcidin through GDF-15 in Cancer-Related Anemia
Fang Jiang et al,Clinica Chimica Acta,2014
靶點(diǎn)詳情
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最新研究進(jìn)展:
GDF15蛋白是一種細(xì)胞因子,最近的研究發(fā)現(xiàn)其在代謝性疾病、腫瘤、神經(jīng)退行性疾病等多個(gè)方面都具有重要的生物學(xué)作用。例如,在腫瘤中,GDF15蛋白的表達(dá)水平常常與腫瘤的惡性程度和預(yù)后相關(guān);在代謝性疾病中,GDF15蛋白的表達(dá)水平可以作為預(yù)測(cè)心血管事件和死亡的生物標(biāo)志物。此外,研究還表明,GDF15蛋白還參與了體內(nèi)能量代謝的調(diào)節(jié)以及神經(jīng)元發(fā)生和功能的調(diào)節(jié)等生物學(xué)過(guò)程。
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功能:
Regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Binds to its receptor, GFRAL, and activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem. It then triggers the activation of neurons localized within the parabrachial nucleus and central amygdala, which constitutes part of the 'emergency circuit' that shapes feeding responses to stressful conditions. On hepatocytes, inhibits growth hormone signaling.
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基因功能參考文獻(xiàn):
- Data indicate that LINC01133 inhibited OSCC metastasis via a feedback regulation loop of reciprocal inhibition with GDF15. PMID: 30332510
- Our results provide valuable evidence that GDF15 is related with first-line chemo-resistance, with highly expressed GDF15 being a strong and an independent indicator of shorter PFS in EOC patients. PMID: 29580231
- Placenta and appetite genes GDF15 and IGFBP7 are associated with hyperemesis gravidarum. PMID: 29563502
- These results suggest that upregulation of NAG-1 contributes to trichostatin A-induced apoptosis in human endometriotic stromal cells. PMID: 29157123
- GDF-15 is a promising biomarker for prediction of HF and death due to CHD in the general population, which may provide prognostic information to already established clinical biomarkers. PMID: 29771963
- the association between GDF-15 and contrast-induced nephropathy in acute myocardial infarction patients after percutaneous coronary intervention PMID: 29791474
- Age-dependent GDF-15 centile values developed from a population of apparently healthy older adults are independently predictive of all-cause mortality PMID: 29714603
- To the best of our knowledge, this is the first documentation of cytokine GDF-15 presence in follicular fluid. Its concentration was not associated with donor/patient fertility status. Our data also show that GDF-15 is expressed and inducible in both normal healthy and cancerous granulosa cells, as well as in oocytes PMID: 29948426
- Findings indicate that global platelet function was positively correlated with serum GDF-15 concentration. PMID: 28597461
- GDF-15 in blood is more accurate in differentiating pancreatic mass due to chronic pancreatitis from pancreatic adenocarcinoma than Ca19-9. the combination of GDF-15 with Ca125 is remarkably useful in differentiating pancreatic mass. PMID: 29171983
- Our results show GDF-15 as an effective biomarker in patients with metastatic colorectal cancer PMID: 29400662
- This study found that the mean serum GDF15 levels in the Parkinson Disease patients were significantly higher than those of the healthy control group. Secondly, in the Parkinson Disease patients, the GDF15 levels were significantly higher in the men than in the women. PMID: 28787735
- Elevated GDF-15 correlates with lack of reverse remodeling and increased mortality after TAVR and improves risk prediction of mortality. PMID: 29222133
- Initial serum GDF15 level showed an inverse correlation with serum glomerular filtration rate and was associated with worse renal outcome in immunoglobulin A nephropathy. PMID: 28869715
- data reveal that YAP promotes metastasis of breast cancer cells by repressing GDF15 transcription and present a novel molecular mechanism underlying the pro-metastasis function of YAP oncoprotein, with the implication of a therapeutic avenue for breast cancer treatment. PMID: 29499325
- GDF15 was significantly correlated with mitochondrial disease severity and the proportion of ragged-red fibers identified in skeletal muscle. PMID: 27889897
- GDF-15 serum levels were elevated in patients with Systemic sclerosis and correlated with the extent of skin fibrosis, and it was found to be higher in Systemic sclerosis patients with interstitial pulmonary fibrosis PMID: 28872977
- Enhanced radiosensitivity and decreased migration in cells caused by CDP138 knockdown are partially dependent on GDF15 inhibition. PMID: 28880265
- these results suggest that increased GDF-15-as observed in lungs of smokers and patients with COPD-contributes to cigarette smoke-induced pulmonary inflammation. PMID: 28145442
- High GDF15 levels are associated with increased risk of major amputation and/or death in peripheral arterial disease patients. PMID: 28855167
- we found that GDF-15 -3148C/G polymorphism (CC genotype) might have a correlation with acute ST-elevation myocardial infarction occurrence and the formation of collateral circulation. PMID: 29489980
- Data show that serum growth differentiation factor 15 (GDF15)levels were significantly higher in subjects with thyroid nodules compared with nodule negative subjects, and this was influenced by age. PMID: 28489602
- Findings suggest that GDF15 induces tumor sphere formation through GDF15-ERK1/2-GDF15 circuits, leading to maintenance of GDF15high cancer stem-like cells. PMID: 28206960
- These authors demonstrate that blocking cardiomyocyte production of GDF15 normalizes circulating GDF15 level and restores liver growth hormone signaling, establishing GDF15 as a bona fide heart-derived hormone that regulates pediatric body growth. PMID: 28572090
- Authors demonstrated that GDF15 contributed to radioresistance of HNC, as determined by both gain- and lost-of-functional experiments. Authors further showed that GDF15 facilitated the conversion of cancer stemness, as assessed by the promotion of CD44+ and ALDH1+ cell populations and spheroid cell formation. PMID: 27903972
- this study, for the first time, demonstrates that GDF15 is a promising target for preventing cold I/R injury in heart transplantation. This study also shows that the resultant protective effects are mediated by the Foxo3 and NFkappaB signaling pathways PMID: 28388574
- High circulating GDF-15 levels are predictive of secondary cardiovascular events in women but not in men with carotid atherosclerosis. PMID: 28389123
- In patients with chronic obstructive pulmonary disease, high levels of GDF15 were independently associated with a higher yearly rate of exacerbations, higher mortality and increased decline in both FEV1 and FVC. PMID: 28298399
- Data suggested NAG-1 protein as a crucial mediator of epithelial ovarian cancer (EOC) progression and resistance to the standard first-line chemotherapy against EOC, particularly in MyD88-positive ovarian cancer stem-like cells. PMID: 27708225
- The GDF-15 gene rs1804826G/T polymorphism and sGDF-15 levels are associated with ischemic stroke in the Chinese population. PMID: 28526273
- NSAID-activated gene 1 (NAG-1) has been assessed for its involvement in cellular integrity and pathogenesis in mucosal inflammation and carcinogenesis. [review] PMID: 28455268
- showed association between high level of GDF-15, determined at the first 24 hours after acute coronary syndrome, and chronic heart failure progression after 12 months. PMID: 29099703
- Results provide evidence that GDF15 contributes to the cancer-associated fibroblasts-mediated chemoprotection of AML cells. PMID: 27643489
- serum GDF15 was not altered in the first trimester in women destined to develop late-onset preeclampsia. PMID: 27173615
- even very small amounts of contaminating TGF-b in purified protein batches may influence the experimental results given that receptors for TGF-b are present. When we attempted to characterize possible receptors for the TGF-b superfamily ligand GDF15, striking similarities between GDF15-induced activities and known TGF-b activities were found. PMID: 29161287
- In ever-smokers with COPD free of clinical CVD, GDF-15 contributes independently to subclinical coronary atherosclerosis. TRIAL REGISTRATION: ClinicalTrials.gov, NCT00608764 . Registered 28 January 2008. PMID: 28245821
- This is the first study showing that adolescent male swimmers have higher PTX3 levels than sedentary controls and that there is a linear relationship between PTX3 and GDF-15. PMID: 28664745
- GDF-15 at 1 month after an acute coronary syndrome is related to the risk of bleeding during dual antiplatelet therapy and provides additional information on the bleeding risk beyond baseline GDF-15 levels. PMID: 28411246
- NF-kappaB/GDF-15 regulatory axis is important for tumor cells in evading macrophage immune surveillance during the early stages of tumorigenesis PMID: 28891811
- Our data indicated that GDF-15 plays an important role in iron metabolism in SAA. GDF-15 might be a novel target for SAA therapy. PMID: 28385068
- These results suggest that rs1054564 confers allele-specific translational repression of GDF15 via hsa-miR-1233-3p. PMID: 28806401
- Serum MIC-1 levels in PUL were not able to categorically diagnose EP, however, MIC-1 could distinguish women with an EP that required medical intervention and those women whose PULs spontaneously resolved PMID: 23824055
- HIF-1alpha and GDF15 expression are inversely related under normoxia and hyperoxia. PMID: 28734801
- PPIX concentrations correlated with both growth differentiation factor (GDF) 15 (p = 0.012) and male gender (p = 0.015). PMID: 28185024
- Data show that growth differentiation factor 15 (GDF15) knockdown significantly inhibited the growth and metastasis of cancer stem-like sphere cells (SCs) through AKT/GSK-3beta/beta-catenin pathway suppression. PMID: 28199981
- epithelial restitution was promoted by enhanced NAG-1 expression and subsequent enterocyte locomotion during the early wound-healing process PMID: 27421482
- serum GDF15 may be a useful diagnostic and prognostic biomarker for colorectal cancer. PMID: 26990020
- These findings highlight an important contribution of GDF15 in promoting airway epithelial senescence upon cigarette smoke exposure. PMID: 27093475
- The results of this study, including a robust induction of GDF-15 expression by several external factors known to elevate IOP, and rGDF-15-induced increase in contractility, cell adhesion, and the levels of ECM proteins and alphaSMA in TM cells PMID: 27918822
- GDF-15 captures distinct aspects of cardiovascular disease development, progression, and prognosis, which are not represented by clinical risk predictors and other biomarkers. The usefulness of GDF-15 to guide management decisions and discover new treatment targets should be further explored. PMID: 28062617
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亞細(xì)胞定位:
Secreted.
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蛋白家族:
TGF-beta family
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組織特異性:
Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney. Detected in plasma (at protein level).
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數(shù)據(jù)庫(kù)鏈接:
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