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  4. Human heme oxygenase 2,HO-2 ELISA Kit

Human heme oxygenase 2,HO-2 ELISA Kit

  • 中文名稱：
    人血紅素氧合酶2(HO-2)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08408h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人血紅素氧合酶2(HO-2)酶聯(lián)免疫試劑盒（CSB-E08408h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液樣本中的HMOX2含量。HMOX2，全稱血紅素加氧酶2，是一種關(guān)鍵酶，催化血紅素氧化裂解，釋放一氧化碳和膽綠素，同時(shí)釋放二價(jià)鐵。研究顯示，HMOX2基因在藏族高原低氧適應(yīng)中發(fā)揮作用，調(diào)節(jié)血紅蛋白代謝，實(shí)現(xiàn)低氧環(huán)境下的遺傳適應(yīng)。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，該產(chǎn)品廣泛用于基礎(chǔ)醫(yī)學(xué)研究，例如探索氧化應(yīng)激相關(guān)疾病的分子機(jī)制、評(píng)估藥物或基因干預(yù)對(duì) HO - 2 表達(dá)的影響，或用于細(xì)胞模型中炎癥及代謝調(diào)控通路的分析，為科研人員提供高效可靠的工具。適用于科研場(chǎng)景中 HO - 2 的表達(dá)水平分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Heme oxygenase (decycling) 2 ELISA Kit; Heme oxygenase (decyclizing) 2 ELISA Kit; Heme oxygenase 2 ELISA Kit; HMOX 2 ELISA Kit; Hmox2 ELISA Kit; HMOX2 protein ELISA Kit; HMOX2_HUMAN ELISA Kit; HO 2 ELISA Kit; HO-2 ELISA Kit; HO2 ELISA Kit; OTTHUMP00000159847 ELISA Kit
  • 縮寫(xiě)：
    HMOX2
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates
  • 檢測(cè)范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human HO-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 89
    Range % 84-95
    1:2 Average % 92
    Range % 87-101
    1:4 Average % 98
    Range % 94-104
    1:8 Average % 98
    Range % 93-105
  • 回收率：
    The recovery of human HO-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 97 93-102
    EDTA plasma (n=4) 91 84-97
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    20 2.447 2.292 2.370 2.222
    10 1.959 1.820 1.890 1.742
    5 1.161 1.251 1.206 1.058
    2.5 0.706 0.763 0.735 0.587
    1.25 0.406 0.416 0.411 0.263
    0.625 0.290 0.298 0.294 0.146
    0.312 0.221 0.223 0.222 0.074
    0 0.144 0.152 0.148 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.
  • 基因功能參考文獻(xiàn)：
    1. The frequencies of genotype and allelic variants of ALAD rs1800435 did not differ significantly between patients with essential tremor (ET) and controls, and were not influenced by gender. Subjects carrying the ALAD rs1800435CC genotype (wild-type) and the HMOX2 rs1051308GG genotype or the HMOX2 rs1051308G allele had significantly decreased risk for ET. PMID: 28276576
    2. HO-2 is a cellular myristate-binding protein that negatively regulates both virus replication and host inflammatory responses. PMID: 28132836
    3. Our results suggest that rs1051308 is associated with risk of developing Parkinson disease in Han Chinese, and further studies involving various ethnicities are needed to validate the association. PMID: 28179208
    4. High HMOX2 expression is associated with bladder cancer. PMID: 28320388
    5. HMOX2 contributes to high-altitude adaptation in Tibetans by functioning as a modifier in the regulation of hemoglobin metabolism. PMID: 26781569
    6. a weak association between HMOX2 rs1051308 polymorphisms and the risk to develop essential tremor in the Spanish population. PMID: 26091465
    7. Taken together with EPR measurements, which show the appearance of a new low-spin heme signal in reduced HO2, it appears that a cysteine residue(s) in the HRMs directly interacts with a second bound heme PMID: 25849895
    8. HO-2 protein is expressed in the cytosols of skin cancer cells. PMID: 25864768
    9. Interactions of HO-2 with CPR and BVR, were evaluated. PMID: 25196843
    10. increased expression of nucleated RBC, HSP90alpha and corresponding decreased expression of HO-2 in such hypoxic condition may play a protective role; to prevent cord blood RBC against stress induced damage during preeclampsia. PMID: 22935040
    11. PFKFB4 and HO-2 are expressed in a coordinated manner to maintain glucose homeostasis. PMID: 22892400
    12. Role of cysteine residues in heme binding to human heme oxygenase-2 elucidated by two-dimensional NMR spectroscopy. PMID: 22923613
    13. Although the carboxy-terminal deletion mutant of HO-2 is found in the nucleus, translocation of HO-2 to the nucleus does not occur under conditions of hypoxia. PMID: 22545110
    14. for the first time, copy number variations in the HMOX2 gene and an association of the SNP rs2270363 with Parkinson's disease risk. PMID: 21709601
    15. Results suggest that the c.544G>A polymorphism of the heme oxygenase-2 gene is not associated with age-related macular degeneration in this population. PMID: 21804464
    16. HO-2, which is highly expressed in the corneal epithelium, appears to be critical for the wound healing process in the cornea. PMID: 21506105
    17. These findings are consistent with the presence of a hydrogen-bonding network at the heme's distal side within the active site of HO-2 with potentially significant differences from that observed in HO-1. PMID: 20502928
    18. There was positive correlation between seminal plasma HO enzyme activity and sperm concentration, per cent of motile spermatozoa, number of motile spermatozoas ml(-1) and significant negative correlation with per cent of sperm abnormal forms. PMID: 20629646
    19. high expression in keratinocytes prevents basal and radiation-induced gene expression of heme oxygenase 1 PMID: 19874887
    20. HO-2 is important in maintaining endothelial viability and may preserve local regulation of vascular tone, thrombosis, and inflammatory responses during reductions in systemic oxygen delivery PMID: 20118244
    21. HO-2 protein content was decreased by 17% and 5% in human trophoblast cells after 24-h exposure to 1% and 5% O(2), respectively, versus 20% O(2) but unchanged in chorionic villi PMID: 12578814
    22. Low expression of HO-2 may lead to enhanced levels of free heme at the feto-maternal interface, with subsequent upregulation of adhesion molecules, allowing enhanced inflammatory cells migration to the feto-maternal interface. PMID: 14506930
    23. HO-2 is part of the BK channel complex and enhances channel activity in normoxia PMID: 15528406
    24. Catalytically inactive mutant, HO-2H45A, overexpressed in HEK293 cell lines was more sensitive to hemin as compared to control. HO-2H45A was also able to protect cells against oxidative stress injury. PMID: 16043027
    25. Review summarizes function of hemoxygenase-2 as an oxygen sensor of native and recombinant large conductance, voltage- and calcium-dependent potassium BK(Ca) channels expressed in carotid body glomus cells. PMID: 16137652
    26. These results suggest that HO-2 may down-regulate the expression of HO-1, thereby directing the co-ordinated expression of HO-1 and HO-2. PMID: 17064313
    27. Suggest membrane potential gradient in small intestine is dependent on carbon monoxide generated by HO-2 in interstital cells of Cajal. PMID: 17510199
    28. the heme regulatory motifs in HO-2 constitute a thiol/disulfide redox switch that regulates the myriad physiological functions of HO-2, including its involvement in the hypoxic response in the carotid body PMID: 17540772
    29. analysis of apo- and heme-bound crystal structures of a truncated human heme oxygenase-2 PMID: 17965015
    30. HO-2 may be important in controlling trophoblast invasion. PMID: 19345412
    31. The thiol/disulfide switch in HO-2 responds to cellular oxidative stress and reductive conditions, representing a paradigm for how heme regulatory motifs can integrate heme homeostasis with carbon monoxide signaling and redox regulation. PMID: 19473966

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  • 亞細(xì)胞定位：
    Microsome. Endoplasmic reticulum.
  • 蛋白家族：
    Heme oxygenase family
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 5014

    OMIM: 141251

    KEGG: hsa:3163

    STRING: 9606.ENSP00000219700

    UniGene: Hs.284279