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  4. Human interleukin-18 binding prorein(IL-18BP) ELISA Kit

Human interleukin-18 binding prorein(IL-18BP) ELISA Kit

  • 中文名稱：
    人白介素18結(jié)合蛋白(IL-18BP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13687h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人白介素18結(jié)合蛋白(IL-18BP)酶聯(lián)免疫試劑盒（CSB-E13687h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的IL18BP含量。IL18BP即白介素18結(jié)合蛋白，它可與IL-18結(jié)合阻斷其活性。IL-18參與機體免疫調(diào)節(jié)、炎癥反應(yīng)等。IL18BP作為內(nèi)源性抑制劑，調(diào)控IL-18活性，有潛力用于炎癥相關(guān)疾病如關(guān)節(jié)炎、感染性疾病的治療研究。試劑盒檢測范圍為15.6 pg/ml-1000 pg/ml，靈敏度為3.9 pg/ml。適用于基礎(chǔ)醫(yī)學(xué)研究領(lǐng)域，例如探究炎癥相關(guān)疾病的分子機制、評估免疫調(diào)節(jié)藥物對IL - 18/IL - 18BP信號通路的影響，或作為生物標志物在心血管疾病、代謝綜合征等病理模型中的動態(tài)監(jiān)測工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    I18BP_HUMAN ELISA Kit; IL-18BP ELISA Kit; IL18 binding protein ELISA Kit; IL18 BP ELISA Kit; IL18 BPa ELISA Kit; IL18BP ELISA Kit; IL18BPa ELISA Kit; Interleukin 18 binding protein ELISA Kit; Interleukin-18-binding protein ELISA Kit; MC51L 53L 54L homolog gene product ELISA Kit; Tadekinig alfa ELISA Kit; Tadekinig-alfa ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    15.6 pg/ml-1000 pg/ml
  • 靈敏度：
    3.9 pg/ml
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

    　

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(pg/ml)

    128.430

    124.396

    125.740

    124.844

    128.205

    126.861

    SD

    0.032

    0.035

    0.037

    0.050

    0.046

    0.047

    CV(%)

    4.819

    5.418

    5.675

    7.716

    6.938

    7.154

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-18BP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:10

    Average %

    100

    Range %

    95-104

    1:20

    Average %

    84

    Range %

    80-88

    1:40

    Average %

    98

    Range %

    92-103

    1:80

    Average %

    90

    Range %

    85-97

  • 回收率：

    The recovery of human IL-18BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    90-100

    EDTA plasma (n=4)

    93

    89-98

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    1000

    2.412

    2.624

    2.518

    2.412

    500

    1.845

    1.952

    1.899

    1.793

    250

    1.181

    1.124

    1.153

    1.047

    125

    0.685

    0.645

    0.665

    0.559

    62.5

    0.371

    0.393

    0.382

    0.276

    31.2

    0.234

    0.223

    0.229

    0.123

    15.6

    0.169

    0.162

    0.166

    0.060

    0

    0.107

    0.105

    0.106

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

平均分:
0.0分 - 1 個評價

樣品類型：血漿（抗凝劑） EDTA

樣品信息：人

稀釋比：其他 1:20

產(chǎn)品評價： 我用CSB-E13687h檢測系統(tǒng)性紅斑狼瘡病人血漿樣本中的IL-18BP, 標準品OD范圍是0.06-1.75. 血漿樣本OD值范圍是0.8-1.5，標準曲線R平方約0.99, 依據(jù)標準曲線算出的樣本濃度范圍是8-17ng/mL, 符合預(yù)期結(jié)果。

By 唐老師

靶點詳情

  • 功能：
    Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response.
  • 基因功能參考文獻：
    1. these results were corroborated in female osteoporotic subjects where decreased serum IL-18BP levels and enhanced serum IL-18 levels were observed. Our study forms a strong basis for using humanized IL-18BP towards the treatment of postmenopausal osteoporosis. PMID: 27649785
    2. epigenetic silencing by single CpG methylation determines differential IL18BP inducibility in monocytic versus epithelial cells. T PMID: 29409936
    3. a large proportion blood basophils from patients with asthma express IL-18 and IL-18BP. mast cells and basophils are implicated in the pathogenesis of asthma via an IL-18-related mechanism. PMID: 29505668
    4. The maintenance of normal production of IL-18BP may contribute, at least in part, to the ability ofLong Term Non-Progressors to delay AIDS progression. PMID: 27863336
    5. Platelets also contain the IL-18 antagonist, the IL-18-Binding Protein (IL-18BP); however, it is not synthesized in them de novo, is present in pre-made form and is released irrespective of platelet activation. PMID: 27914933
    6. The serum levels of IL-37, which were correlated with antibody production and the serum levels of total IL-18 and IL-18BP, were elevated in the patients with primary Sjogren's syndrome. PMID: 28057714
    7. an imbalance in the production of IL-18 and its antagonist (an increase in the production of IL-18 with a decrease, no increase or an insufficient increase in the production of IL-18BP) has been described in many chronic inflammatory diseases in humans. PMID: 26898120
    8. IL-18, IL-18R and IL-18BP expression in eosinophil are involved in the inflammatory reaction of asthma. PMID: 28395725
    9. the inhibition of IL-18 signaling by IL-18BPa may be involved in the development of pulmonary vascular involvement leading to pulmonary hypertension and modulate the systemic inflammation in systemic sclerosis PMID: 26777734
    10. The natural IL-18 inhibitor IL-18BP further regulates IL-18 activity in the extracellular environment. Review. PMID: 25548255
    11. imbalance of IL-18/IL-18BP ratio in IgA nephropathy especially in patients with arteriolar lesions PMID: 25807634
    12. IL-18, IL-18BP, and IL-18R may have roles in the pathogenesis of epithelial ovarian carcinoma PMID: 24963217
    13. HPV16 E7 oncoprotein increases production of the IL18BP in keratinocytes. PMID: 24478434
    14. these data indicate that IL-18BP, which is produced in EOC in response to microenvironmental factors, may inhibit endogenous or exogenous IL-18 activity PMID: 23873689
    15. Data suggest that the imbalance of IL-18/IL-18BP might play an important role in the pathogenesis of systemic juvenile idiopathic arthritis (SJIA) and it might go hand-in-hand with the severity of SJIA. PMID: 23419721
    16. Due to highly significant increases in circulating IL-18BP in schizophrenia compared to controls, the levels of free IL-18 are not significantly different between schizophrenic groups. PMID: 22913567
    17. Imbalance between IL-18 and IL-18BP may play an important role in pathogenesis of idiopathic thrombocytopenic purpura. PMID: 21867627
    18. High IL-18 BP levels indicate the severity of existing glomerular injury in systemic lupus erythematosus. PMID: 21656327
    19. The balance between interleukin-18 (IL-18) and its endogenous antagonist, IL-18 binding protein (IL-18BP), was evaluated in children with Henoch-Schonlein purpura. PMID: 22289535
    20. IL-18/IL-18BP imbalance plays an important role in pathogenesis of primary immune thrombocytopenia. PMID: 21418867
    21. In T2DM patients, 18 BP began to increase after IL-18 increased and reached a threshold, in which case kidney dysfunction would have developed. PMID: 21440322
    22. increased levels of both IL-18 and its natural inhibitor IL-18BP, characterise SLE PMID: 21126942
    23. In lines of intestinal epithelial cells from inflammatory bowel disease patients, interferon-gamma selectively up-regulated IL-18 binding protein. PMID: 21078084
    24. lupus nephritis patients present lower IL-18BP mRNA expression and higher serum levels of IL-18 than those in normal controls PMID: 20140691
    25. Altogether, data presented herein indicate that direct action of STAT1 on the IL-18BP promoter at the proximal GAS element is key to IL-18BP expression by IFN-gamma-stimulated DLD-1 colon carcinoma cells PMID: 19046253
    26. High circulating levels in patients with active Systemic Lupus Erythematosus PMID: 19699611
    27. Endometrial interleukin-18 binding protein mRNA expression may possibly be responsible for the pathologic process of adenomyosis. PMID: 19394601
    28. Suggest that an early rise in IL-18 levels may play a role in reverse remodeling process following aortic valve replacement. PMID: 19961286
    29. Since IL-18 plays a major role in perpetuating hemophagocytosis, the failure of IFNgamma to induce IL-18BP may constitute a fundamental pathogenetic mechanism PMID: 20072626
    30. trancriptional activation and release of IL-18 binding protein in response to IFN-gamma PMID: 11739524
    31. IL-18BP messenger transcript and protein are significantly increased in surgically resected specimens from active Crohn's disease compared with control patients, correlating with an up-regulation of IL-18. PMID: 11907126
    32. after binding to IL-18BP, IL-1F7b forms a complex with IL-18Rbeta, depriving the beta-chain of forming a functional receptor complex with IL-18Ralpha and thus inhibiting IL-18 activity PMID: 12381835
    33. IRF-1-CEBPbeta complex activate the promoter of IL-18 binding protein. PMID: 12482935
    34. Administration of recombinant human IL-18BP not only reduces symptoms of murine contact hypersensitivity (CHS) during the elicitation phase but also significantly decreases inflammation in mice that had previously undergone CHS without treatment. PMID: 12874202
    35. High endogenous levels of IL-18BP in trangenic mice effectively neutralize IL-18 and are protective in response to different inflammatory stimuli. PMID: 12960225
    36. production of IL-18BP in response to IL-12 and IL-18 was regulated differently in blood and synovial cells. PMID: 15188356
    37. IL-18 levels, which are determined in part by variation in IL18/IL18BP, play a role in coronary heart disease development and postsurgery outcome. PMID: 17951325
    38. Despite the elevated IL-18BP levels during active disease, free IL-18 remained higher than in the inactive disease stages, suggesting a potential benefit of administration of exogenous IL-18BP as a therapeutic approach for active Wegener's granulomatosis. PMID: 18594952

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  • 亞細胞定位：
    Secreted.
  • 組織特異性：
    Strongly expressed in heart, lung, placenta and spleen.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 5987

    OMIM: 604113

    KEGG: hsa:10068

    STRING: 9606.ENSP00000260049

    UniGene: Hs.591967