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Human irisin ELISA kit

  • 中文名稱:
    人鳶尾素酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-EQ027943HU
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人鳶尾素酶聯(lián)免疫試劑盒(CSB-EQ027943HU)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的irisin含量。Irisin是一種由運(yùn)動(dòng)誘導(dǎo)產(chǎn)生的肌肉因子。背景上,它被視為運(yùn)動(dòng)益處的重要介質(zhì)。研究機(jī)制方面,Irisin能促進(jìn)白色脂肪棕色化,增加能量消耗,還參與調(diào)節(jié)葡萄糖代謝、骨代謝等過程,在代謝性疾病等領(lǐng)域有潛在治療價(jià)值。試劑盒檢測(cè)范圍為3.12 ng/mL-200 ng/mL,科研人員可將其應(yīng)用于運(yùn)動(dòng)生理學(xué)、代謝調(diào)控機(jī)制、脂肪組織功能研究等領(lǐng)域,通過定量分析不同干預(yù)條件下(如運(yùn)動(dòng)訓(xùn)練、藥物處理)樣本中鳶尾素濃度變化,為探索代謝相關(guān)疾病的分子機(jī)制提供可靠數(shù)據(jù)支持。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    irisin
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    3.12 ng/mL-200 ng/mL
  • 靈敏度:
    0.78 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Others
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human irisin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:1 Average % 90    
    Range % 84-96    
    1:2 Average % 97    
    Range % 91-103    
    1:4 Average % 103    
    Range % 97-109    
    1:8 Average % 89    
    Range % 83-95    
  • 回收率:
    The recovery of human irisin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 100 94-106    
    EDTA plasma (n=4) 106 101-112    
                   
                   
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    200 2.690 2.752 2.721 2.616    
    100 2.402 2.371 2.387 2.282    
    50 1.965 1.853 1.909 1.804    
    25 1.355 1.247 1.301 1.196    
    12.5 0.764 0.735 0.750 0.645    
    6.25 0.495 0.464 0.480 0.375    
    3.12 0.251 0.266 0.259 0.154    
    0 0.106 0.103 0.105      
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
    Irisin是一種由肌肉細(xì)胞產(chǎn)生的激素,在身體代謝調(diào)節(jié)中發(fā)揮著重要作用。最近的研究表明,Irisin對(duì)改善糖尿病、肥胖和心血管疾病等慢性病的預(yù)防和治療具有潛在價(jià)值。此外,研究人員正在探索Irisin對(duì)其他疾病的影響,如阿爾茨海默病、肌肉萎縮和骨質(zhì)疏松癥等。雖然Irisin的作用機(jī)制還沒有完全理解,但這種激素已經(jīng)成為新的藥物開發(fā)領(lǐng)域的熱門研究方向之一。