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  4. Human leucine-rich alpha-2 glycoprotein 1(LRG1) ELISA Kit

Human leucine-rich alpha-2 glycoprotein 1(LRG1) ELISA Kit

  • 中文名稱：
    人富亮氨酸α2糖蛋白1(LRG1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E12962h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人富亮氨酸α2糖蛋白1(LRG1)酶聯(lián)免疫試劑盒（CSB-E12962h）為競爭法ELISA試劑盒，定量檢測血清、尿液、組織勻漿樣本中的LRG1含量。試劑盒檢測范圍為0.156 μg/mL-40 μg/mL，科研人員可通過研究LRG1在心血管疾病、糖尿病腎病、腫瘤轉(zhuǎn)移等病理過程中的表達動態(tài)，分析其與疾病進展的相關(guān)性，或用于藥物干預(yù)實驗中的生物標志物檢測本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    1300008B03Rik ELISA Kit; 2310031E04Rik ELISA Kit; A2GL ELISA Kit; A2GL_HUMAN ELISA Kit; HMFT1766 ELISA Kit; Leucine rich alpha 2 glycoprotein ELISA Kit; Leucine-rich alpha-2-glycoprotein 1 ELISA Kit; Leucine-rich alpha-2-glycoprotein ELISA Kit; Leucine-rich alpha-2-glycoprotein precursor ELISA Kit; LRG ELISA Kit; LRG1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, urine, tissue homogenates
  • 檢測范圍：
    0.156 μg/mL-40 μg/mL
  • 靈敏度：
    0.156 μg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<12%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<15%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human LRG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    89

    Range %

    84-95

    1:2

    Average %

    90

    Range %

    85-95

    1:4

    Average %

    95

    Range %

    90-102

    1:8

    Average %

    92

    Range %

    87-97

  • 回收率：

    The recovery of human LRG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    91

    87-96

    EDTA plasma (n=4)

    95

    91-100

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    μg/ml

    OD1

    OD2

    Average

    40

    0.132

    0.134

    0.133

    10

    0.340

    0.357

    0.348

    2.5

    0.655

    0.641

    0.648

    0.625

    0.959

    0.965

    0.962

    0.156

    1.469

    1.479

    1.474

    0

    2.038

    2.063

    2.051

  • 本試劑盒所含材料：
    • A 96-well Assay plate --The 96-well plate has been pre-coated with LRG1.
    • Standard (2 x 250 μl)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
    • Anti-human LRG1 antibody (100 x concentrate) (1 x 60 μl)
    • Antibody Diluent (1 x 10 ml) --Dilute the anti-human LRG1
    • HRP-conjugated goat-anti-mouse LRG1antibody (100 x concentrate) (1 x 120 μl) --Act as the detection antibody.
    • HRP-conjugate Diluent (1 x 20 ml) --Dilute the HRP-conjugated LRG1
    • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
    • Wash Buffer (25x concentrate) (1 x 20 ml)--Wash away unbound or free substances.
    • TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells)
    • An Instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 基因功能參考文獻：
    1. LRG-1 was highly expressed in human retinoblastoma sections, thus providing new insights into the molecular mechanism of retinoblastoma pathogenesis, and suggests a possible new therapeutic target. PMID: 29392314
    2. The detection of LRG1 in accordance with the DeltaRct value (electron-transfer resistance at the electrode surface) of the sensor layer incorporating LRG1 BP3 peptides showed a statistically significant difference (p < 0.001) between adenomas and carcinomas, indicating that the potential use of this biosensing platform for detecting the CRC biomarker, as well as for monitoring the colorectal adenoma-to-carcinoma tra... PMID: 28697446
    3. This study shows that TNF-alpha is the predominant proinflammatory cytokine that induces the secretion of LRG1. LRG1 contributes to angiogenesis-coupled de novo bone formation by increasing angiogenesis and recruiting MSCs in the subchondral bone of osteoarthritis joints. PMID: 28358372
    4. the expression of 3 miRs and 9 mRNAs associate with the PIK3CA status. Expression of LRG1 is independent of luminal (A or B) subtype, decreased after neo-adjuvant aromatase inhibitor treatment. PMID: 27491861
    5. Plasma LRG1 predicts both albuminuria and Chronic kidney disease progression beyond traditional risk factors. It may play a role in the pathologic pathway leading to progression of diabetic kidney disease in T2 Diabetes mellitus. PMID: 28973352
    6. LRG1 may be a key regulatory factor of allergic responses. PMID: 27378305
    7. Results find serum LRG1 levels are significantly associated with LRG1 expression in gastric tumors. Its inhibition suppresses gastric cancer cell growth and invasion suggesting that LRG1 is implicating in gastric tumorigenesis. PMID: 28746773
    8. LRG1 plays a crucial role in the proliferation and apoptosis of colorectal cancer (CRC) by regulating RUNX1 expression. LRG1 may be a potential detection biomarker as well as a marker for monitoring recurrence and therapeutic target for CRC. PMID: 28376129
    9. These findings suggest that sputum LRG is a promising biomarker of local inflammation in asthma. PMID: 27611322
    10. combination of modified whole blood LRG1 mRNA levels, plasma LRG1 protein and Alvarado score at the ED may be useful to diagnose simple and complicated acute appendicitis from other causes of abdominal pain PMID: 28202345
    11. Downregulation of LRG1 is associated with head and neck squamous cell carcinoma. PMID: 28098902
    12. Results suggest that serum LRG-1 is a promising biomarker for pancreatic cancer. PMID: 25058884
    13. Results provided evidence for LRG1 function as a novel inducer of epithelial-mesenchymal transition and angiogenesis in colorectal cancer, which was at least partially through promotion of HIF-1alpha expression. PMID: 26856989
    14. Data show that leucine-rich-alpha-2-glycoprotein 1 (LRG1) is markedly up-regulated and serves as an independent factor of poor outcomes in hepatocellular carcinoma (HCC). PMID: 26517349
    15. Reanalysis of a total of 47 MDS patients revealed biomarker candidates, with alpha-2-HS-glycoprotein and leucine-rich alpha-2-glycoprotein as the most promising candidates. PMID: 26448929
    16. Two of these proteins were verified in a separate patient cohort: values of CRP and LRG1 combined gave a highly significant indication of extended survival post one week of radiotherapy treatment. PMID: 26425690
    17. Findings suggest that LRG1 promotes invasion and migration of glioma cells through TGF-beta signaling pathway. PMID: 26049667
    18. LGR1 is involved in the inhibition of HCC metastasis. PMID: 25814288
    19. silencing the expression of LRG1 suppresses the growth of glioblastoma U251 cells in vitro and in vivo, suggesting that LRG1 may play a critical role in glioblastoma development, and it may have potential clinical implications in glioblastoma therapy. PMID: 25589464
    20. Higher LRG1 is a significant predictor for arterial stiffness, endothelial function, and peripheral arterial disease. PMID: 25636050
    21. The blood proteins IGF1, IGF2, IBP2, IBP3 and A2GL have been proposed as indirect biomarkers for detection of GH administration and as putative biomarkers for breast cancer diagnosis. PMID: 25277046
    22. LRG1 potentially offered clinical value in directing personal treatment for endometrial carcinoma patients PMID: 24760273
    23. Human LRG (hLRG) concentration in the cerebrospinal fluid (CSF) was measured and hLRG expression in post-mortem human cerebral cortex and mouse LRG (mLRG) in the brain were examined. PMID: 24058569
    24. Overexpression of serum LRG1 is associated with non-small cell lung cancer. PMID: 23284758
    25. High Serum leucine-rich alpha-2 glycoprotein is associated with ulcerative colitis. PMID: 22374925
    26. Increased plasma levels of the APC-interacting protein MAPRE1, LRG1, and IGFBP2 precede a diagnosis of colorectal cancer in women PMID: 22277732
    27. This study demonistrated that LRG expression in resident astrocytes increased with age. PMID: 22116432
    28. Screened for NSCLC-related proteins in urinary exosomes by comparing urinary exosomes proteome of normal controls and NSCLC patients. The LRG1 was found to be expressed at higher levels in urinary exosomes and lung tissue of NSCLC patients. PMID: 21557262
    29. LRG1 was identified as a serum biomarker that accurately identifies patients with heart failure. PMID: 21282491
    30. autologous Cyt c is an endogeneous ligand for LRG and PLIbeta and these serum proteins neutralize the autologous Cyt c released from the dead cells PMID: 20442399
    31. cDNA clones for human leucine-rich alpha2-glycoprotein were isolated. LRG expression was up-regulated during neutrophil differentiation but down-regulated during monocytic differentiation. The hLRG gene was localized to chromosome 19p13.3. PMID: 12223515
    32. Mass spectrometry analysis identified the serum inhibitory factor as leucine-rich alpha-2-glycoprotein-1 in enzyme-linked immunosorbent assay . PMID: 16699948
    33. These results also indicate that the CSF level assay of TGF-beta1, TbetaR-II and LRG is useful for the diagnosis of patients with INPH, and TGF-beta1, TbetaR-II and LRG may be involved in the pathogenesis of the disease. PMID: 17194537
    34. Several indirect lines of evidence suggest a role for LRG in implantation/decidualization. PMID: 17583700
    35. These results strongly suggest that LRG was a secretory type 1 acute-phase protein whose expression was up-regulated by the mediator of acute-phase response. PMID: 19324010

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  • 亞細胞定位：
    Secreted.
  • 組織特異性：
    Plasma.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 29480

    OMIM: 611289

    KEGG: hsa:116844

    STRING: 9606.ENSP00000302621

    UniGene: Hs.655559