Human leucocyte antigen B27,HLA-B27 ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人類白細(xì)胞抗原B27(HLA-B27)酶聯(lián)免疫試劑盒（CSB-E08607h）為雙抗夾心法ELISA試劑盒，定量檢測血清、尿液、腦脊液（CSF）樣本中的HLA-B27含量。HLA-B27是人類白細(xì)胞抗原B27。它與脊柱關(guān)節(jié)病尤其是強直性脊柱炎密切相關(guān)。研究機制上，HLA-B27可能通過分子模擬、關(guān)節(jié)源性肽及免疫調(diào)節(jié)異常等機制，引發(fā)機體異常免疫反應(yīng)，導(dǎo)致關(guān)節(jié)等部位的炎癥與病變。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，可為免疫性疾病機制研究、炎癥反應(yīng)通路探索以及HLA - B27表達(dá)水平與疾病相關(guān)性分析提供可靠工具，尤其適用于神經(jīng)免疫、風(fēng)濕病學(xué)等領(lǐng)域的體液樣本檢測需求，其多樣本類型的兼容性為跨組織液比較研究創(chuàng)造了便利條件。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

HLA-B27
Uniprot No.：

P03989
種屬：

Homo sapiens (Human)
樣本類型：

serum, urine, cerebrospinal fluid (CSF)
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human HLA-B27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	80-98
1:2	Average %	86
	Range %	80-96
1:4	Average %	96
	Range %	92-100
1:8	Average %	100
	Range %	96-110

回收率：

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.