Human midregional pro-atrial natriuretic peptide (MR-proANP) ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (3) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人中部前心鈉肽(MR-proANP)酶聯(lián)免疫試劑盒（CSB-EQ028056HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的MR-proANP含量。試劑盒檢測(cè)范圍為39 pmol/L-2500 pmol/L，該產(chǎn)品適用于心血管疾病機(jī)制研究、動(dòng)物模型中心功能評(píng)估、藥物干預(yù)效果監(jiān)測(cè)等科研領(lǐng)域，為探索心力衰竭、心肌缺血等疾病的分子機(jī)制提供定量分析工具。試劑盒操作流程標(biāo)準(zhǔn)化，配套完整實(shí)驗(yàn)組分，支持實(shí)驗(yàn)室開(kāi)展高通量樣本檢測(cè)，滿足基礎(chǔ)醫(yī)學(xué)研究和生物標(biāo)志物開(kāi)發(fā)需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

MR-proANP
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

39 pmol/L-2500 pmol/L
靈敏度：

9.8 pmol/L
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human MR-proANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	85-97
1:2	Average %	100
	Range %	94-105
1:4	Average %	90
	Range %	83-94
1:8	Average %	97
	Range %	91-101

回收率：

The recovery of human MR-proANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	102	92-107

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.