Human neutrophil peptide 1-3,HNP1-3 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人中性粒細(xì)胞防御素1-3(HNP1-3)酶聯(lián)免疫試劑盒（CSB-E11742h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的HNP1-3含量。試劑盒檢測(cè)范圍為1.56 ng/mL-100 ng/mL，適用于探索感染性疾病機(jī)制、免疫調(diào)控研究、炎癥相關(guān)疾病模型建立等科研場(chǎng)景，例如膿毒癥、類風(fēng)濕性關(guān)節(jié)炎或腫瘤微環(huán)境中的免疫應(yīng)答研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

HNP1-3
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human HNP1-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-102
1:2	Average %	85
	Range %	81-90
1:4	Average %	95
	Range %	87-99
1:8	Average %	100
	Range %	85-106

回收率：

The recovery of human HNP1-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	95	87-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.