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  3. 酶聯(lián)免疫試劑盒
  4. Human opiorphin ELISA kit
  • 中文名稱(chēng)：
    人opiorphin酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EQ027423HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人opiorphin酶聯(lián)免疫試劑盒（CSB-EQ027423HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、唾液樣本中的opiorphin含量。opiorphin是一種內(nèi)源性阿片肽增強(qiáng)劑。其背景在于它是人體天然存在的物質(zhì)。研究機(jī)制方面，它能抑制阿片肽的降解，增強(qiáng)阿片肽活性，從而發(fā)揮鎮(zhèn)痛、抗焦慮等生理作用，在疼痛治療和神經(jīng)精神疾病領(lǐng)域具有潛在研究?jī)r(jià)值。試劑盒檢測(cè)范圍為0.625 ng/mL-40 ng/mL，適用于基礎(chǔ)科研中鎮(zhèn)痛機(jī)制探索、精神疾病模型構(gòu)建及神經(jīng)肽動(dòng)態(tài)監(jiān)測(cè)等方向。助力科研人員深入探究 Opiorphin 在生理病理過(guò)程中的調(diào)控作用。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    opiorphin
  • 種屬：
    Homo sapiens (Human)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates, saliva
  • 檢測(cè)范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線(xiàn)性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human opiorphin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %89
    Range %82-92
    1:2Average %98
    Range %93-103
    1:4Average %92
    Range %87-96
    1:8Average %92
    Range %86-97
  • 回收率：
    The recovery of human opiorphin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)8681-92
  • 標(biāo)準(zhǔn)曲線(xiàn)：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    402.561 2.669 2.615 2.491
    202.205 2.114 2.160 2.036
    101.457 1.354 1.406 1.282
    50.742 0.720 0.731 0.607
    2.50.425 0.405 0.415 0.291
    1.250.275 0.283 0.279 0.155
    0.6250.187 0.181 0.184 0.060
    00.123 0.124 0.124
  • 本試劑盒所含材料：
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human opiorphin antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled opiorphin antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問(wèn)答

■ 常見(jiàn)問(wèn)題解答
Q:

Regarding your ELISA kit CSB-EQ027423HU, could you please advise on the following:
1. Is the standard natural material or recombinant? And if recombinant, what kind of cell or living material is it used for expression?
2. Does the standard include additive agents such as antiseptic or preservative? If included, what is the composition of said additive agent(s)?
3. Material name and concentration of Stop Solution.
4. Do these kits include hazardous substance such as azide, thimerosal, 2-ME, etc?

A:
Thanks for your inquiry!
CSB-EQ027423HU
Standard is synthetic peptide.
Preservative is proclin 300(1:3000)；
Stop solution is 4N sulfuric acid.
This kit doesn't contain azide, thimerosal, 2-ME, etc.