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  4. Human osteonectin,ON ELISA Kit

Human osteonectin,ON ELISA Kit

  • 中文名稱：
    人骨粘連蛋白(ON)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08760h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人骨粘連蛋白(ON)酶聯(lián)免疫試劑盒（CSB-E08760h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、唾液、組織勻漿樣本中的SPOCK2含量。試劑盒檢測(cè)范圍為0.78 ng/mL-50 ng/mL，適用于科研場(chǎng)景下探究骨發(fā)育、心血管鈣化、腫瘤轉(zhuǎn)移等生物學(xué)過程中ON蛋白的動(dòng)態(tài)變化，尤其適合多類型生物樣本的批量檢測(cè)需求，滿足實(shí)驗(yàn)室對(duì)骨基質(zhì)相關(guān)蛋白的高通量定量分析要求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    and Kazal-like domains proteoglycan 2 ELISA Kit; CWCV ELISA Kit; FLJ97039 ELISA Kit; KIAA0275 ELISA Kit; SPARC/osteonectin ELISA Kit; Sparc/osteonectin cwcv and kazal like domains proteoglycan (testican) 2 ELISA Kit; Sparc/osteonectin cwcv and kazal like domains proteoglycan 2 ELISA Kit; SPOCK 2 ELISA Kit; Spock2 ELISA Kit; Testican 2 ELISA Kit; Testican-2 ELISA Kit; Testican2 ELISA Kit; TICN 2 ELISA Kit; TICN2 ELISA Kit; TICN2_HUMAN ELISA Kit
  • 縮寫：
    SPOCK2
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, saliva, tissue homogenates
  • 檢測(cè)范圍：
    0.78 ng/mL-50 ng/mL
  • 靈敏度：
    0.195 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of Human ON in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:20Average %94
    Range %87-98
    1:40Average %96
    Range %90-102
    1:80Average %94
    Range %88-98
    1:160Average %97
    Range %92-103
  • 回收率：
    The recovery of Human ON spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9894-102
    EDTA plasma (n=4)8680-92
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    502.658 2.857 2.758 2.663
    252.307 2.208 2.258 2.163
    12.51.601 1.543 1.572 1.477
    6.250.847 0.867 0.857 0.762
    3.120.485 0.474 0.480 0.385
    1.560.307 0.319 0.313 0.218
    0.780.181 0.188 0.185 0.090
    00.094 0.095 0.095
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    May participate in diverse steps of neurogenesis. Binds calcium.
  • 基因功能參考文獻(xiàn)：
    1. candidate susceptibility gene for bronchopulmonary dysplasia; lung expression pattern indicates potential role in alveolarization PMID: 21836138
    2. Neurons are a major source of all of the testican family members in the normal brain. Abundant distribution of testican 2 may contribute to glioma invasion by inactivating other testican family members including N-Tes, which all inhibit MT-MMPs. PMID: 12810672
    3. purification and characterization of a recombinant testican-2 expressed in insect cells; the C1q subcomponent of complement component C1 was identified as a potential interacting partner of testican-2 PMID: 17950619
  • 亞細(xì)胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 組織特異性：
    Highly expressed in brain. Also found in lung and testis.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 13564

    OMIM: 607988

    KEGG: hsa:9806

    STRING: 9606.ENSP00000321108

    UniGene: Hs.523009