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  4. Human peroxiredoxin 2 (PRDX2) ELISA kit

Human peroxiredoxin 2 (PRDX2) ELISA kit

  • 中文名稱：
    人過(guò)氧化物酶2(PRDX2)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL018654HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人過(guò)氧化物酶2(PRDX2)酶聯(lián)免疫試劑盒（CSB-EL018654HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的PRDX2含量。試劑盒檢測(cè)范圍為47 pg/mL-3000 pg/mL，適用于基礎(chǔ)研究中PRDX2表達(dá)水平的動(dòng)態(tài)監(jiān)測(cè)、疾病模型氧化損傷評(píng)估、藥物干預(yù)效果分析及信號(hào)通路機(jī)制探索等方向，為細(xì)胞實(shí)驗(yàn)、動(dòng)物模型及臨床前研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    47 pg/mL-3000 pg/mL
  • 靈敏度：
    11.75 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PRDX2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %85
    Range %81-95
    1:2Average %97
    Range %91-105
    1:4Average %96
    Range %92-108
    1:8Average %93
    Range %86-102
  • 回收率：
    The recovery of human PRDX2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9289-98
    EDTA plasma (n=4)9792-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    30002.3812.4192.400 2.253
    15001.9351.9521.944 1.797
    7501.4271.4031.415 1.268
    3751.0681.0591.064 0.917
    187.50.6270.650.639 0.492
    940.4120.4230.418 0.271
    470.2950.280.288 0.141
    00.1490.1450.147 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    Peroxiredoxin family, AhpC/Prx1 subfamily
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 9353

    OMIM: 600538

    KEGG: hsa:7001

    STRING: 9606.ENSP00000301522

    UniGene: Hs.432121