Human platelet-derived growth factor CC (PDGF-CC) ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人血小板衍生生長因子CC(PDGF-CC)酶聯(lián)免疫試劑盒（CSB-E13241h）為雙抗夾心法ELISA試劑盒，定量檢測血清、組織培養(yǎng)上清液、尿液、組織勻漿樣本中的PDGF-CC含量。PDGF-CC 即血小板衍生生長因子 CC，在細胞生長、增殖等生理過程中發(fā)揮作用。其異常表達與腫瘤、纖維化等疾病的發(fā)生發(fā)展相關(guān)。研究機制主要聚焦于其信號通路，阻斷該信號有望為相關(guān)疾病治療提供方向，目前是研究熱門靶點。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，科研人員可通過該產(chǎn)品開展PDGF-CC在疾病模型中的表達水平監(jiān)測、藥物干預(yù)效果評估或信號通路機制研究，為心血管生物學(xué)、腫瘤微環(huán)境及再生醫(yī)學(xué)等領(lǐng)域的體外實驗提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

PDGF-CC
種屬：

Homo sapiens (Human)
樣本類型：

serum, cell culture supernates, urine, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PDGF-CC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	95
	Range %	92-99
1:4	Average %	94
	Range %	89-101
1:8	Average %	89
	Range %	84-95

回收率：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.