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  4. Human pro-gastrin-releasing peptide, ProGRP ELISA Kit

Human pro-gastrin-releasing peptide, ProGRP ELISA Kit

  • 中文名稱：
    人胃泌素釋放肽前體(ProGRP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E09127h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人胃泌素釋放肽前體(ProGRP)酶聯(lián)免疫試劑盒（CSB-E09127h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ProGRP含量。ProGRP（胃泌素釋放肽前體）是一種在小細(xì)胞肺癌（SCLC）中特異性高表達(dá)的腫瘤標(biāo)志物。它作為SCLC發(fā)生發(fā)展中的生物標(biāo)志物，可能具有致癌基因的作用，并可能成為潛在的治療靶點(diǎn)。研究顯示，ProGRP在SCLC的診斷、療效評估和預(yù)后預(yù)測中具有重要作用。試劑盒檢測范圍為18.75 pg/mL-1200 pg/mL，適用于腫瘤標(biāo)志物篩選、疾病機(jī)制研究及抗腫瘤藥物開發(fā)等科研領(lǐng)域，尤其為肺癌相關(guān)生物標(biāo)志物探索、病理模型驗(yàn)證及組織微環(huán)境分析提供可靠工具，其優(yōu)化的檢測體系支持多樣本類型的高通量檢測需求，滿足實(shí)驗(yàn)室基礎(chǔ)研究與轉(zhuǎn)化醫(yī)學(xué)應(yīng)用。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    ProGRP
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    18.75 pg/mL-1200 pg/mL
  • 靈敏度：
    4.68 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ProGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:5Average %87
    Range %80-90
    1:10Average %97
    Range %92-101
    1:20Average %98
    Range %93-102
    1:40Average %93
    Range %86-96
  • 回收率：
    The recovery of human ProGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-96
    EDTA plasma (n=4)9791-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    12002.432 2.327 2.380 2.253
    6001.894 1.886 1.890 1.763
    3001.271 1.237 1.254 1.127
    1500.721 0.692 0.707 0.580
    750.467 0.452 0.460 0.333
    37.50.338 0.345 0.342 0.215
    18.750.238 0.225 0.232 0.105
    00.129 0.125 0.127 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)