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  4. Human procollagen Ⅰ N-terminal peptide,PⅠNP ELISA Kit

Human procollagen Ⅰ N-terminal peptide,PⅠNP ELISA Kit

  • 中文名稱：
    人Ⅰ型前膠原N端前肽(PⅠNP)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E11226h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人Ⅰ型前膠原N端前肽(PⅠNP)酶聯(lián)免疫試劑盒（CSB-E11226h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的PⅠNP含量。PⅠNP即I型前膠原氨基端前肽，它是反映成骨細(xì)胞活性和骨形成的特異性指標(biāo)。其背景在于可用于評(píng)估骨代謝狀態(tài)。研究機(jī)制上，主要是通過檢測(cè)血液中PⅠNP水平，來反映骨合成代謝過程，輔助診斷和監(jiān)測(cè)骨質(zhì)疏松癥等骨病。試劑盒檢測(cè)范圍為18.75 pg/mL-1200 pg/mL，適用于骨組織工程研究、骨骼疾病動(dòng)物模型評(píng)估、骨代謝相關(guān)分子機(jī)制解析等科研場(chǎng)景，為骨代謝研究、抗骨質(zhì)疏松藥物篩選及骨再生材料開發(fā)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    procollagen 1 N-terminal peptide,P1NP,_x000D_ procollagen I N-terminal peptide,PINP
  • 縮寫：
    PⅠNP
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    18.75 pg/mL-1200 pg/mL
  • 靈敏度：
    4.68 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PⅠNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 97  
    Range % 89-102  
    1:200 Average % 90  
    Range % 83-97  
    1:400 Average % 99  
    Range % 94-105  
    1:800 Average % 96  
    Range % 90-102  
  • 回收率：
    The recovery of human PⅠNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-99  
    EDTA plasma (n=4) 101 95-107  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1200 2.191 2.287 2.239 2.086  
    600 1.885 1.896 1.891 1.738  
    300 1.527 1.507 1.517 1.364  
    150 1.001 1.042 1.022 0.869  
    75 0.692 0.648 0.670 0.517  
    37.5 0.499 0.472 0.486 0.333  
    18.75 0.272 0.288 0.280 0.127  
    0 0.152 0.154 0.153 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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