Human progranulin(PGRN) ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人前顆粒蛋白(PGRN)酶聯(lián)免疫試劑盒（CSB-E15049h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液樣本中的PGRN含量。試劑盒檢測范圍為4.7 ng/mL-300 ng/mL，支持科研人員通過定量檢測PGRN在細(xì)胞模型中的分泌動(dòng)態(tài)，或探索其在神經(jīng)系統(tǒng)疾病、癌癥等研究領(lǐng)域的生物標(biāo)志物價(jià)值，也可用于評(píng)估藥物干預(yù)或基因修飾對(duì)PGRN表達(dá)的影響，為分子機(jī)制研究提供可靠工具，適用于基礎(chǔ)科研和體外生物醫(yī)學(xué)研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

PGRN
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates
檢測范圍：

4.7 ng/mL-300 ng/mL
靈敏度：

1.17 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PGRN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	103
	Range %	97-110
1:2	Average %	97
	Range %	92-101
1:4	Average %	90
	Range %	85-94
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of human PGRN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	89	84-93

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.