Human soluble fibrin monomer complex,SFMC ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人可溶性血纖蛋白單體復(fù)合物(SFMC)酶聯(lián)免疫試劑盒（CSB-E09097h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液樣本中的SFMC含量。試劑盒檢測范圍為6.25 μg/mL-400 μg/mL，適用于實驗室研究凝血與纖溶系統(tǒng)動態(tài)平衡、構(gòu)建血栓性疾病動物模型后的生物標(biāo)志物分析，或探索尿液中SFMC與腎臟相關(guān)疾病的潛在關(guān)聯(lián)性研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

SFMC
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, urine
檢測范圍：

6.25 μg/mL-400 μg/mL
靈敏度：

1.56 μg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human SFMC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	81-92
1:2	Average %	100
	Range %	95-106
1:4	Average %	96
	Range %	92-100
1:8	Average %	89
	Range %	83-95

回收率：

The recovery of human SFMC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	80-91
EDTA plasma (n=4)	99	95-103

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.