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  4. Human sperm associated antigen 9 (SPAG9) antibody ELISA Kit

Human sperm associated antigen 9 (SPAG9) antibody ELISA Kit

  • 中文名稱：
    人精子相關(guān)抗原9(SPAG9) 抗體酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13940h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人精子相關(guān)抗原9(SPAG9) 抗體酶聯(lián)免疫試劑盒（CSB-E13940h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的SPAG9 Ab含量。試劑盒檢測范圍為50 mU/mL-1200 mU/mL，本試劑盒特別適用于生殖生物學(xué)基礎(chǔ)研究、男性不育相關(guān)分子機(jī)制探索、生殖系統(tǒng)疾病模型構(gòu)建等科研領(lǐng)域本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    SPAG9 Ab
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    50 mU/mL-1200 mU/mL
  • 靈敏度：
    20 mU/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<10%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human SPAG9 antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 90  
    Range % 87-98  
    1:2 Average % 93  
    Range % 91-99  
    1:4 Average % 95  
    Range % 90-102  
    1:8 Average % 92  
    Range % 86-98  
  • 回收率：
    The recovery of human SPAG9 antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 86-98  
    EDTA plasma (n=4) 96 91-100  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/ml OD1 OD2 Average Corrected  
    1200 2.004 1.949 1.977 1.930  
    600 1.334 1.318 1.326 1.279  
    300 0.772 0.783 0.778 0.731  
    100 0.351 0.370 0.361 0.314  
    50 0.223 0.212 0.218 0.171  
    0 0.047 0.046 0.047    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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