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  4. Human tumor necrosis factor-related apoptosis-inducing ligand receptor 4(TRAIL-R4) ELISA Kit

Human tumor necrosis factor-related apoptosis-inducing ligand receptor 4(TRAIL-R4) ELISA Kit

  • 中文名稱(chēng)：
    人腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體受體4(TRAIL-R4)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E04749h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體受體4(TRAIL-R4)酶聯(lián)免疫試劑盒（CSB-E04749h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的TNFRSF10D含量。TNFRSF10D是一個(gè)靶點(diǎn)。它在腫瘤免疫等領(lǐng)域有重要背景，可參與調(diào)節(jié)免疫細(xì)胞活性等過(guò)程。其研究機(jī)制方面，主要圍繞它與配體相互作用、下游信號(hào)通路傳導(dǎo)展開(kāi)，影響細(xì)胞增殖、凋亡等，為相關(guān)疾病治療研究提供了方向。試劑盒檢測(cè)范圍為0.156 ng/ml- 10 ng/ml，靈敏度為0.039 ng/ml。該產(chǎn)品適用于腫瘤生物學(xué)、免疫調(diào)控機(jī)制等基礎(chǔ)研究領(lǐng)域，可用于評(píng)估細(xì)胞模型、動(dòng)物實(shí)驗(yàn)或臨床前研究中TRAIL-R4的表達(dá)動(dòng)態(tài)，輔助探究其在腫瘤微環(huán)境中的作用或藥物干預(yù)效果，例如化療藥物敏感性測(cè)試、凋亡信號(hào)通路研究等場(chǎng)景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    CD 264 ELISA Kit; CD264 ELISA Kit; DcR 2 ELISA Kit; DcR2 ELISA Kit; Decoy receptor 2 ELISA Kit; Decoy with truncated death domain ELISA Kit; OTTHUMP00000123528 ELISA Kit; TNF receptor related receptor for TRAIL ELISA Kit; TNF related apoptosis inducing ligand receptor 4 ELISA Kit; TNF-related apoptosis-inducing ligand receptor 4 ELISA Kit; TNFRSF 10D ELISA Kit; TNFRSF10D ELISA Kit; TR10D_HUMAN ELISA Kit; TRAIL R4 ELISA Kit; TRAIL receptor 4 ELISA Kit; TRAIL receptor with a truncated death domain ELISA Kit; TRAIL-R4 ELISA Kit; TRAILR4 ELISA Kit; TRUNDD ELISA Kit; Tumor necrosis factor receptor superfamily member 10D ELISA Kit; Tumor necrosis factor receptor superfamily; member 10d; decoy with truncated death domain ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.156 ng/ml - 10 ng/ml
  • 靈敏度：
    0.039 ng/ml
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線(xiàn)性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human TRAIL-R4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    89

    Range %

    86-92

    1:2

    Average %

    95

    Range %

    91-99

    1:4

    Average %

    98

    Range %

    92-104

    1:8

    Average %

    92

    Range %

    82-95

  • 回收率：

    The recovery of human TRAIL-R4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    90-101

    EDTA plasma (n=4)

    107

    102-110

  • 標(biāo)準(zhǔn)曲線(xiàn)：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    10

    2.214

    2.323

    2.269

    2.112

    5

    1.597

    1.486

    1.542

    1.385

    2.5

    0.957

    0.886

    0.922

    0.765

    1.25

    0.547

    0.568

    0.558

    0.401

    0.625

    0.355

    0.366

    0.361

    0.204

    0.312

    0.281

    0.297

    0.289

    0.132

    0.156

    0.208

    0.214

    0.211

    0.054

    0

    0.156

    0.157

    0.157

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Receptor for the cytotoxic ligand TRAIL. Contains a truncated death domain and hence is not capable of inducing apoptosis but protects against TRAIL-mediated apoptosis. Reports are contradictory with regards to its ability to induce the NF-kappa-B pathway. According to PubMed:9382840, it cannot but according to PubMed:9430226, it can induce the NF-kappa-B pathway.
  • 基因功能參考文獻(xiàn)：
    1. The high-dose hook effect was apparent during ELISA testing of uDcR2 in chronic kidney disease (CKD) patients, yet dilution of the urine samples neutralized this effect. However, the use of a four-fold dilution of urine for uDcR2/cre testing may eliminate the high-dose hook effect and make it possible to effectively monitor the severity of TII in CKD patients. PMID: 29879421
    2. results suggest that CD264 is a surface marker of cellular age for bone marrow-derived mesenchymal stem cells. PMID: 28962588
    3. Urinary DcR2 could potentially serve as a novel biomarker for tubulointerstitial injury and may reflect senescence of renal proximal tubular cells in diabetic nephropathy. PMID: 28356293
    4. Results identified epigenetic inactivation of TNFRSF10C and TNFRSF10D in majority of cervical cancer cases. PMID: 26542757
    5. membrane expression more common in endometrioid endometrial cancer than in normal endometrium PMID: 24649804
    6. This study identified TNFRSF10D DNA methylation status as an independent prognostic biomarker for relapse-free survival and overall mortality in non-metastatic melanoma patients. PMID: 25003639
    7. The membrane expression of the TRAIL receptors DR4, DR5, DcR1 and DcR2 is greater in normal endometrium than endometrioid adenocarcinoma (EAC). The level of the receptors in EAC is not dependent on grading and staging and does not predict survival. PMID: 23584885
    8. the results presented here claim for a relevant impact of aberrant methylation of decoy receptors in melanoma and allow to understand how the silencing of DcR1 and DcR2 is related to melanomagenesis. PMID: 24211571
    9. TRAIL receptor-4 expression profiles on T cells might be important in revelation of rheumatoid arthritis pathogenesis. PMID: 20799941
    10. ANT2 shRNA treatment sensitized MCF7, T47 D, and BT474 cells to TRAIL-induced apoptosis by up-regulating the expression of TRAIL death receptors 4 and 5 (DR4 and DR5) and down-regulating the TRAIL decoy receptor 2 (DcR2). PMID: 20875141
    11. these results demonstrated that hypoxia-inducible factor 1alpha played a crucial role in regulating the transcription of DcR2. PMID: 20018172
    12. Enhanced expression of DcR2 promotes peripheral blood eosinophil survival in the airways of allergic asthmatics following segmental antigen challenge. PMID: 12421985
    13. Cytotoxicity and apoptosis induced by TRAIL to beta-cell lines CM were inhibited competitively by soluble TRAIL receptors, R1, R2, R3 or R4. PMID: 12488957
    14. Respiratory syncytial virus infection strongly up-regulated the expression of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and its functional receptors death receptor 4 (DR4) and DR5. PMID: 12915532
    15. Our results demonstrate that DcR1 and DcR2 genes are frequently methylated in various tumor types and aberrant methylation was the cause for silencing of DcR1 and DcR2 expression. PMID: 14999791
    16. The DcR2 was found to have a truncated and non-functional death domain. PMID: 15538968
    17. CD8+ lymphocytes and NKT lymphocytes, but not CD4+ lymphocytes, express TRAIL-R4 PMID: 15919363
    18. Resistance to TRAIL-induced apoptosis in acute myeloid leukemia cells is associated with expression of TRAIL-R4. PMID: 15921376
    19. Preligand assembly domain-mediated ligand-independent association between TRAIL receptor 4 (TR4) and TR2 regulates TRAIL-induced apoptosis. PMID: 16319225
    20. DCR2 was found positive in 81 and 33% normal, 46 and 10% nodular hyperplasia, 74 and 36% PIN tissues, 87 and 89% low-grade carcinomas, and 100 and 93% high-grade carcinomas PMID: 16799475
    21. TRAIL-R4-beta is a new splice variant of TRAIL-receptor 4 lacking the cysteine rich domain 1 PMID: 16934748
    22. The specificity of DcR1- and DcR2-mediated TRAIL inhibition reveals an additional level of complexity for the regulation of TRAIL signaling. PMID: 16980609
    23. CASP8, DCR2, and HIN-1 methylation leads to progression of neuroblastoma PMID: 17545522
    24. These data strongly support a recent proposal that a segment at 8p21.3 contains crucial prostate cancer tumor suppressors. PMID: 18460741
    25. DCR2-methylated patients showed significantly poorer 5-year event-free survival in the whole neuroblastoma group (43% PMID: 18980997
    26. High TRAIL death receptor 4 and decoy receptor 2 expression correlates with significant cell death in pancreatic ductal adenocarcinoma patients. PMID: 18981952
    27. Results indicate that disc cells, after herniation, undergo apoptotic cell death via the DR5/TRAIL pathway. PMID: 19730199

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  • 亞細(xì)胞定位：
    Membrane; Single-pass type I membrane protein.
  • 組織特異性：
    Widely expressed, in particular in fetal kidney, lung and liver, and in adult testis and liver. Also expressed in peripheral blood leukocytes, colon and small intestine, ovary, prostate, thymus, spleen, pancreas, kidney, lung, placenta and heart.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 11907

    OMIM: 603614

    KEGG: hsa:8793

    STRING: 9606.ENSP00000310263

    UniGene: Hs.213467