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  4. Human α2 macroglobulin,α2 MG ELISA Kit

Human α2 macroglobulin,α2 MG ELISA Kit

  • 中文名稱：
    人α2巨球蛋白(α2-MG)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08959h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人α2巨球蛋白(α2-MG)酶聯(lián)免疫試劑盒（CSB-E08959h）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的A2M含量。A2M（α2-巨球蛋白）是一種血漿中含量豐富的大分子糖蛋白，具有廣譜蛋白酶抑制活性，參與宿主防御機制。其研究機制涉及與多種細(xì)胞因子、生長因子和激素結(jié)合，調(diào)節(jié)體內(nèi)平衡，發(fā)揮保護作用。在奶牛乳腺炎抗性研究中，A2M基因的突變可增強其表達活性，提高奶?？共×?。試劑盒檢測范圍為1.25 μg/mL-20 μg/mL，靈敏度為1.25 μg/mL。適用于科研領(lǐng)域中對α2 - MG表達水平的研究，例如炎癥相關(guān)疾病機制探索、免疫調(diào)控分析或組織損傷修復(fù)模型中的生物標(biāo)志物檢測，支持多種生物樣本的靈活檢測需求，為體外實驗提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    A2m ELISA Kit; A2MG_HUMAN ELISA Kit; Alpha 2 M ELISA Kit; Alpha 2M ELISA Kit; Alpha-2-M ELISA Kit; Alpha-2-macroglobulin ELISA Kit; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 ELISA Kit; CPAMD5 ELISA Kit; DKFZp779B086 ELISA Kit; FWP007 ELISA Kit; S863 7 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.25 μg/mL-20 μg/mL
  • 靈敏度：
    1.25 μg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human α2 MG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 87  
    Range % 82-93  
    1:200 Average % 94  
    Range % 89-99  
    1:400 Average % 95  
    Range % 89-99  
    1:800 Average % 92  
    Range % 87-96  
  • 回收率：
    The recovery of human α2 MG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 85-98  
    EDTA plasma (n=4) 96 92-101  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    20 0.185 0.187 0.186    
    10 0.360 0.365 0.363    
    5 0.635 0.642 0.639    
    2.5 1.163 1.172 1.168    
    1.25 1.995 2.013 2.004    
    0 2.849 2.924 2.887    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 最新研究進展：
        α2-巨球蛋白（α2-MG）是一種血漿蛋白，在人體各種生理和病理狀態(tài)下發(fā)揮著多種生物學(xué)功能。以下是α2-MG的最新研究進展：
        α2-MG在免疫調(diào)節(jié)中的作用受到廣泛關(guān)注。研究表明，α2-MG可以通過抑制T細(xì)胞、B細(xì)胞和單核細(xì)胞的活化和增殖，降低細(xì)胞因子的產(chǎn)生，從而發(fā)揮抗炎和免疫調(diào)節(jié)的作用。此外，α2-MG還可以促進某些免疫細(xì)胞的增殖和活化，增強免疫應(yīng)答。
        α2-MG在腫瘤生物學(xué)中也具有重要作用。研究表明，α2-MG的表達水平在某些腫瘤中升高，并與腫瘤的發(fā)生、進展和預(yù)后密切相關(guān)。此外，α2-MG還可以調(diào)節(jié)腫瘤細(xì)胞的增殖、轉(zhuǎn)移和凋亡，參與腫瘤微環(huán)境的調(diào)節(jié)。
        α2-MG在心血管疾病中的作用備受關(guān)注。研究表明，α2-MG可以通過抑制血小板的聚集和血管收縮，降低血液黏稠度和血壓，從而發(fā)揮保護心血管系統(tǒng)的作用。此外，α2-MG還可以參與血管生成和修復(fù)，促進血管再生。
        α2-MG在神經(jīng)系統(tǒng)中的作用也得到研究。研究表明，α2-MG可以通過抑制神經(jīng)元凋亡和炎癥反應(yīng)，保護神經(jīng)系統(tǒng)免受損傷。此外，α2-MG還可以影響神經(jīng)元的突觸可塑性和神經(jīng)傳遞，從而調(diào)節(jié)神經(jīng)功能。
  • 功能：
    Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
  • 基因功能參考文獻：
    1. Serum alpha2MG levels might reflect endothelial dysfunction evaluated by flow-mediated dilation in patients with chronic stroke or cardiovascular risk factors PMID: 29093276
    2. The blood coagulation pathway may be a key regulatory network pathway contributing to the allergic inflammatory response in AR patients. A2M, which is regulated by STAT3, may be an important protein in the pathogenesis of allergic rhinitis in AR patients. PMID: 27228572
    3. Proteomics study showed a strong association of FN1, A2M, C4BPA and CFB in molecular subtypes of breast cancer, in which, C4BPA and A2M demonstrated a potent signature in blood plasma and tissue samples of Luminal-B (LB) and Triple-negative (TN)subtypes in BC patients, respectively. PMID: 27498393
    4. BBB disruption is present in ACS, and elevated levels of IL-6 and C3 in CSF in diffuse NPSLE PMID: 29036223
    5. Case Reports: inflammatory myofibroblastic tumors of the lung containing a chimeric A2M-ALK gene categorized as a specific type of inflammatory myofibroblastic tumor that develops exclusively in neonates and infants. PMID: 28705706
    6. The plasma level of alpha2M was found positively correlated, while HCII level was negatively correlated with higher vulnerability of carotid plaques. Both proteins were efficient in differentiating stable and vulnerable carotid plaques. These provide potential new targets for the research of carotid plaque vulnerability. Plasma alpha2M and HCII may be potential biomarkers for evaluation of the vulnerability of carotid ... PMID: 28087279
    7. Stabilizers during pasteurization improved alpha-2-macroglobulin (alpha2-M) activity by more than 300% points compared with the unstabilized control. PMID: 28215695
    8. Data show that low that low globulin (GLB) levels were significantly associated with favorable survival, and low ALB levels were associated with a significantly worse survival . PMID: 26476541
    9. This work demonstrates that alpha2M*/CS-GRP78 acts as an upstream regulator of the PDK1/PLK1 signaling axis to modulate c-MYC transcription and its target genes, suggesting a therapeutic strategy for targeting c-MYC-associated malignant progression. PMID: 27002159
    10. Meta-analysis. Our data indicate that the rs669 (A/G) polymorphisms in A2M gene are associated with increased risk in Parkinson disease. PMID: 25495992
    11. These data demonstrate that alpha2M* and LRP1 modulate the BKCachannel in human myometrium and that BKCaand its immunomodulatory interacting partners regulate Ca(2+)dynamics in hMSMCs during pregnancy. PMID: 27044074
    12. we identify a single serum factor, alpha2-macroglobulin (alpha2M), which is both required and sufficient for rosetting mediated by the parasite protein P. falciparum erythrocyte membrane protein 1 protein HB3VAR06 and some other rosette-mediating PfEMP1 proteins PMID: 26134405
    13. A2M is expressed in the vasculature and NR4A receptors modulate VSMC MMP2/9 activity by several mechanisms including the up-regulation of A2M. PMID: 25809189
    14. Data indicate that the expression of salivary alpha2 macroglobulin (alpha2-M) showed a remarkable increase in type 2 diabetes mellitus (T2DM), which may be associated with functional disorders of the salivary gland. PMID: 25867373
    15. A2M is not the only biological candidate gene for AD determination PMID: 25574746
    16. Alpha2M causes neurodegeneration in a p75(NTR)- and proNGF-dependent manner. PMID: 26217017
    17. there is no enough evidence for associations of A2M gene polymorphisms (5 bp I/D, Ile1000Val) with Alzheimer's disease risk PMID: 24756728
    18. phosphorylating the T-loop Akt residue Thr(308) by PDK1 requires Raptor of the mTORC1 complex as a platform or scaffold protein. PMID: 24516643
    19. genetic variation within the A2M gene influences CSF Ab42 levels. PMID: 24011543
    20. alpha2M is a specialized chaperone that prevents the extracellular accumulation of misfolded and potentially pathogenic proteins, particularly during innate immune system activity PMID: 24799681
    21. FEIgG and alpha2m/C are powerful predictors of outcome and responsiveness to steroids and cyclophosphamide; their predictive value, if validated in prospective studies, may be useful in clinical practice PMID: 24093110
    22. There is no statistically significant difference in allele or genotype frequencies between patients with sporadic Alzheimer's disease and control subjects for the A2M polymorphism. PMID: 23186781
    23. Eosinophil activation occurred in early and late responses after L-ASA NPT in upper airway mucosa of AERD patients, where ApoA1, alpha2M and CP as well as CysLT may be involved in eosinophilic inflammation. PMID: 23786280
    24. A model is proposed that suggests that unlike proteases, which are irreversibly bound to activated alpha2M, hepcidin remains labile and available to down-regulate Fpn1. PMID: 23846698
    25. alpha2macroglobulin stimulates endothelial and early cardiac muscle differentiation of embryonic stem cells through generation of induction of FGF-2 expression. PMID: 23379699
    26. Serum monomeric alpha2-macroglobulin is highly expressed in many diabetic subjects. PMID: 23535567
    27. While activated alpha-2-microglobulin (AA2M) ligates only one GRP78 receptor molecule per AA2M, it may potentially serve as a reservoir for release of up to four binding fragments per molecule. PMID: 23721263
    28. Oxidative stress and the levels of endogenous plasma prolyl oligopeptidase (PREP) inhibitor alpha-2-macroglobulin (alpha2M) decrease PREP activity in patients with multiple sclerosis. PMID: 23643808
    29. IGFBP-2 associates with alpha2-macroglobulin in the blood circulation. PMID: 23397525
    30. [review] alpha2M has many diversified and complex functions but is primarily known by its ability to inhibit a broad spectrum of proteases without the direct blockage of the protease active site. PMID: 23086799
    31. protease-activated alpha2M has two mechanisms for inhibiting amyloid formation: via protease-alpha2M-mediated degradation of amyloidogenic substrates and by a chaperone action that prevents misfolded clients from continuing along the amyloid forming pathway. PMID: 23353684
    32. inhibitory activity of Aeromonas sobria serine protease by alpha2-macroglobulin PMID: 23089609
    33. Complexes between alpha2-macroglobulin and urokinase were detected in alveolar edema fluids as well as in lungs of patients with acute lung injury/acute respiratory distress syndrome where they were located mainly in close proximity to epithelial cells. PMID: 23064953
    34. an isoform of ASP cleaved proteins more in plasma and was inhibited by human alpha(2)-macroglobulin more slowly than single-chain ASP PMID: 22971340
    35. investigation of secondary structure and thermal stability of recombinant bodhesin 2, a spermadhesin from seminal plasma PMID: 22541546
    36. alpha-2-macroglobulin (a2M) deficiency develops from catabolism of a2M in androgen-dependent advanced prostate cancer patients PMID: 22576683
    37. Ethnicity affects the genetic association of A2M with rheumatoid arthritis in South Asian and Caucasian patients living in East Midlands/United Kingdom. PMID: 20401725
    38. We observed that the most informative biomarkers for predicting significant liver fibrosis in chronic hepatitis C patients were blood levels of alpha2-macroglobulin and hyaluronic acid PMID: 21971609
    39. Identify a novel transcriptional regulatory element contributing to the up-regulation of alpha2M during replicative senescence. PMID: 21541797
    40. alpha2-macroglobulin is neurotoxic by inhibiting neuroprotective activity of nerve growth factor via TrkA receptors. PMID: 21642630
    41. dimeric alpha2M as well as tetrameric alpha2M may play an important role in controlling beta2-m amyloid fibril formation PMID: 21216953
    42. The chaperone action of alpha-2-macroglobulin targets prefibrillar species to inhibit amyloid formation PMID: 19074141
    43. The chaperone action of alpha-2-macroglobulin inhibits the toxicity and uptake of A beta in human cerebrospinal fluid PMID: 19472074
    44. The extracellular chaperone alpha-2-macroglobulin is likely to help control amyloid formation and toxicity in vivo PMID: 18075673
    45. Alpha-2-macroglobulin is an extracellular chaperone PMID: 18171086
    46. statistically significant evidence of interaction between the polymorphisms in A2M, SLC6A4 and UCHL1 genes (global P = 0.0107, for the best model) and the risk for PD. PMID: 20861575
    47. LRP1-C/T, A2M-Ile/Val and APOE-epsilon 2/epsilon 3/epsilon 4 polymorphisms are associated with AD. PMID: 20637261
    48. Reduced expression of alpha-2 macroglobulin and complement factor B was detected in sera of patients with nasopharyngeal carcinoma. PMID: 20575108
    49. Haplotype -88G/25G might play a protective role in the development of SAD, and the protective effects of -88G and 25G were independent of APOEvarepsilon4 allele. PMID: 20493925
    50. Galectin-3 Binding Protein and Alpha-2 macroglobulin were differentially expressed on DVT patients in microparticles extracted from platelet-poor plasma PMID: 20156641

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Protease inhibitor I39 (alpha-2-macroglobulin) family
  • 組織特異性：
    Secreted in plasma.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 7

    OMIM: 103950

    KEGG: hsa:2

    STRING: 9606.ENSP00000323929

    UniGene: Hs.212838