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  4. Human β-defensins 3 ELISA Kit

Human β-defensins 3 ELISA Kit

  • 中文名稱：
    人β-防御素3酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E14187h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人β-防御素3酶聯(lián)免疫試劑盒（CSB-E14187h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液、組織培養(yǎng)上清液、組織勻漿樣本中的DEFB103A含量。DEFB103A，也稱為beta Defensin 3，是一種由中性粒細(xì)胞產(chǎn)生的抗菌肽，屬于β-防御素家族。該蛋白在免疫系統(tǒng)中發(fā)揮重要作用，具有抗菌、免疫調(diào)節(jié)和保護(hù)作用。其機(jī)制可能涉及抑制腫瘤生長和增殖，促進(jìn)抗腫瘤免疫反應(yīng)。試劑盒檢測范圍為125 pg/mL-8000 pg/mL，可滿足科研實(shí)驗(yàn)中定量分析hBD-3在疾病模型、免疫調(diào)控機(jī)制或藥物干預(yù)研究中的動態(tài)變化需求，為探索其在感染、自身免疫性疾病或腫瘤微環(huán)境中的作用提供可靠工具；適用于體外樣本的批量檢測，為研究人員評估hBD-3的生物學(xué)功能及相關(guān)通路研究提供高效解決方案本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    BD 3 ELISA Kit; BD-3 ELISA Kit; BD3 ELISA Kit; beta 103 ELISA Kit; Beta defensin 103A ELISA Kit; Beta defensin 103A precursor ELISA Kit; Beta defensin 3 ELISA Kit; Beta-defensin 103 ELISA Kit; Beta-defensin 3 ELISA Kit; D103A_HUMAN ELISA Kit; DEF B3 ELISA Kit; DEFB 103 ELISA Kit; DEFB 103A ELISA Kit; DEFB 3 ELISA Kit; DEFB-3 ELISA Kit; DEFB103 ELISA Kit; DEFB103A ELISA Kit; DEFB103B ELISA Kit; DEFB3 ELISA Kit; Defensin ELISA Kit; Defensin beta 103A ELISA Kit; Defensin beta 3 ELISA Kit; Defensin like protein ELISA Kit; Defensin-like protein ELISA Kit; HBD 3 ELISA Kit; hBD-3 ELISA Kit; HBD3 ELISA Kit; HBP 3 ELISA Kit; HBP3 ELISA Kit; mBD3 ELISA Kit
  • 縮寫：
    DEFB103A
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, urine, cell culture supernates, tissue homogenates
  • 檢測范圍：
    125 pg/mL-8000 pg/mL
  • 靈敏度：
    31.25 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human β-defensins 3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %96
    Range %91-101
    1:2Average %98
    Range %92-104
    1:4Average %92
    Range %87-96
    1:8Average %90
    Range %86-94
  • 回收率：
    The recovery of human β-defensins 3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8782-94
    EDTA plasma (n=4)10298-106
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    80002.339 2.508 2.424 2.270
    40001.833 1.869 1.851 1.697
    20001.185 1.198 1.192 1.038
    10000.699 0.687 0.693 0.539
    5000.375 0.392 0.384 0.230
    2500.294 0.281 0.288 0.134
    1250.208 0.211 0.210 0.056
    00.153 0.155 0.154 　
  • 本試劑盒所含材料：
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human BD3 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled BD3 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

相關(guān)問答

■ 常見問題解答
Q:

We want to use pbs buffer 7.4 as sample instead of serum/plasma etc. Would this affect the results? Can you give us any protocol/suggestion? We need an urgent reply:CSB-E14948h Human Antibacterial Peptide LL-37 ELISA Kit and CSB-E14187h Human β-defensins 3 ELISA Kit.

A:
Thanks for your inquiry.Sorry we did not ever tested PBS 7.4 as sample and we are not sure how it will go on.PBS is mainly composed of phosphate.
Normally,there is no detection target in PBS.May I know if you were trying to say that the customer want to use PBS 7.4 as the sample diluent,not the mentioned serum,plasma sample?The kit is equiped with sample diluent in it and we suggest the customer use the sample diluent in the kit directly.
If further questions,pls let us know.

靶點(diǎn)詳情

  • 功能：
    Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed.
  • 基因功能參考文獻(xiàn)：
    1. this study shows that hBD3 amplifies the response to bacterial DNA in both mouse and human immune cells in a TLR9-dependent manner PMID: 28102569
    2. the role of DEFB103 gene copy number variation (CNV) in ankylosing spondylitis (AS) susceptibility, was investigated. PMID: 26224324
    3. It may be concluded hepatitis B virus up-regulates HBD-3 and A3G expression in vivo and in vitro in placental trophoblast and lack of this up-regulation is possibly associated with intrauterine transmission of hepatitis B. PMID: 25196417
    4. The encoded peptide displays antimicrobial activity against S. aureus and E. faecium. PMID: 11085990
    5. these observations suggest that there may be an interracial difference in DEFB4/103A copy numbers between admixed populations and a relationship between DEFB1 single nucleotide polymorphisms and DEFB4/103A copy number variation PMID: 23194186
    6. Human beta-defensin 3 peptide is increased and redistributed in Crohn's ileitis PMID: 23511030
    7. study demonstrates that HBD2 and 3 activate plasmacytoid dendritic cells by enhancing the intracellular uptake of CpG and self DNA and promote DNA-induced IFN-alpha production in a TLR9-dependent manner PMID: 23776172
    8. Mapping of key residues in the interaction between human Beta-defensin 3 and CXCR4 reveals key defensin motifs involved in protein binding. PMID: 23659571
    9. These results suggest an important role for hBD3 in inducing dendritic cell activation, migration, and polarization. PMID: 22951718
    10. Using a large, unique cohort of pediatric CA-SAB, this study found no significant association between DEFB1 genetic variation or DEFB4/DEFB103 gene copy number and susceptibility for Staphylococcus aureus bacteremia PMID: 22384213
    11. Human beta-defensin 3 exhibits further functions than antimicrobial peptide activity in cultured bone cells, including stimulation of proliferation and differentiation. PMID: 21520074
    12. Data show that high-glucose conditions inhibited the BD3 expression of epidermal keratinocytes. PMID: 21442129
    13. hBD-1 might function as a tumor suppressor gene in oral squamous cell carcinoma, while hBD-2 and -3 might be protooncogenes. PMID: 21280982
    14. extraplacental membranes can react differentialy to the arrival of E. coli, secreting HBD2 and HBD3 mainly in the choriodecidua region. PMID: 21122132
    15. PPARgamma regulates the 1,25D-induced hBD-3 and cathelicidin expression in keratinocytes through the regulation of AP-1 and p38 activity. PMID: 20970965
    16. hBD3 represents a novel NF-kappaB-regulated mediator of CCR7 expression and anti-apoptotic pathways. PMID: 21071608
    17. Inducibility of HBD3 influences the severity of Gram-positive skin infection in humans in vivo. PMID: 20404083
    18. Data show that beta-defensin cluster (DEFB4, DEFB103 and DEFB104) varied between 2 and 9 copies per genome, and high copy numbers (>4) were underrepresented among patients, suggesting that increased copy numbers could protect from CD. PMID: 20483368
    19. Human pulmonary cells produce hBD-3 upon L. pneumophila infection via a TLR-JNK-AP-1-dependent pathway which may contribute to an efficient innate immune defense. PMID: 20615218
    20. The beta-defensin antimicrobial peptides hBD1, hBD2 and hBD3 were expressed by immature dendritic cells as well as gingival epithelial cells. PMID: 20618959

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Beta-defensin family
  • 組織特異性：
    Highly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. Low expression in salivary gland, bone marrow, colon, stomach, polyp and larynx. No expression in small intestine.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 15967

    OMIM: 606611

    KEGG: hsa:414325

    STRING: 9606.ENSP00000324633

    UniGene: Hs.283082