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  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Monkey Complement 3(C3)ELISA Kit

Monkey Complement 3(C3)ELISA Kit

  • 中文名稱：
    猴子補(bǔ)體蛋白3(C3)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E16528Mk
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    猴子補(bǔ)體蛋白3(C3)酶聯(lián)免疫試劑盒（CSB-E16528Mk）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液樣本中的C3含量。C3作為靶點(diǎn)，是補(bǔ)體系統(tǒng)中的關(guān)鍵成分。其在免疫防御等方面發(fā)揮重要作用，異常激活會(huì)引發(fā)多種疾病。研究機(jī)制主要聚焦于調(diào)控C3激活過(guò)程，以此開發(fā)藥物干預(yù)補(bǔ)體過(guò)度激活，為相關(guān)疾病提供新治療策略。試劑盒檢測(cè)范圍為4.68 ng/mL-300 ng/mL，適用于科研領(lǐng)域中對(duì)猴模型（如食蟹猴、恒河猴等）的免疫學(xué)研究，例如評(píng)估疾病模型中補(bǔ)體系統(tǒng)的激活狀態(tài)、藥物或疫苗干預(yù)后免疫應(yīng)答的動(dòng)態(tài)變化，或體外實(shí)驗(yàn)中細(xì)胞因子誘導(dǎo)的補(bǔ)體通路調(diào)控分析本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    C3Complement C3 [Cleaved into: Complement C3 beta chain; Complement C3 alpha chain; C3a anaphylatoxin; C3-beta-c ELISA kit; C3bc); Acylation stimulating protein ELISA kit; ASP ELISA kit; C3adesArg); Complement C3b alpha' chain; Complement C3c alpha' chain fragment 1; Complement C3dg fragment; Complement C3g fragment; Complement C3d fragment; Complement C3f fragment; Complement C3c alpha' chain fragment 2] ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Monkey
  • 樣本類型：
    serum, plasma, cell culture supernates
  • 檢測(cè)范圍：
    4.68 ng/mL-300 ng/mL
  • 靈敏度：
    1.17 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    300 2.276 2.296 2.286 2.144  
    150 1.896 1.854 1.875 1.733  
    75 1.520 1.539 1.530 1.388  
    37.5 1.195 1.174 1.185 1.043  
    18.75 0.864 0.883 0.874 0.732  
    9.37 0.531 0.557 0.544 0.402  
    4.68 0.345 0.365 0.355 0.213  
    0 0.141 0.143 0.142 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.; Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. In chronic inflammation, acts as a chemoattractant for neutrophils.; Acts as a chemoattractant for neutrophils in chronic inflammation.; adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-internalization and recycling of C5AR2.
  • 基因功能參考文獻(xiàn)：
    1. The structure of bovine C3 clearly demonstrates that the main chain around the thioester undergoes a helical transition upon activation, a rearrangement that is proposed to be the basis for the high level of reactivity of the thioester group. PMID: 16831446
  • 亞細(xì)胞定位：
    Secreted.
  • 數(shù)據(jù)庫(kù)鏈接：