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  4. Mouse α-Melanocyte Stimulating Hormone(α-MSH) ELISA Kit

Mouse α-Melanocyte Stimulating Hormone(α-MSH) ELISA Kit

  • 中文名稱：
    小鼠α-黑色素細(xì)胞刺激素(α-MSH)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E15876m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠α-黑色素細(xì)胞刺激素(α-MSH)酶聯(lián)免疫試劑盒（CSB-E15876m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液樣本中的α-MSH含量。α - MSH即α - 促黑素細(xì)胞激素，它是一種內(nèi)源性神經(jīng)肽。背景上，其在體內(nèi)多種生理調(diào)節(jié)中起重要作用。研究機(jī)制方面，它能與相應(yīng)受體結(jié)合，參與調(diào)節(jié)黑素生成、能量代謝、免疫反應(yīng)及炎癥調(diào)控等過程，是當(dāng)前生物學(xué)和醫(yī)學(xué)領(lǐng)域的研究熱點(diǎn)。試劑盒檢測范圍為12.5 pg/mL-800 pg/mL，適用于體外實(shí)驗(yàn)?zāi)Ｐ椭笑?MSH分泌水平的動(dòng)態(tài)監(jiān)測，例如評估藥物干預(yù)對神經(jīng)內(nèi)分泌通路的影響、炎癥模型中肽類激素變化分析，或黑色素細(xì)胞功能相關(guān)的基礎(chǔ)研究本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    alpha-melanocyte stimulating hormone, alpha-MSH
  • 縮寫：
    α-MSH
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates
  • 檢測范圍：
    12.5 pg/mL-800 pg/mL
  • 靈敏度：
    3.12 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Biochemicals
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse α-MSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %89
    Range %86-95
    1:2Average %92
    Range %88-99
    1:4Average %94
    Range %90-99
    1:8Average %93
    Range %87-98
  • 回收率：
    The recovery of mouse α-MSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-97
    EDTA plasma (n=4)9591-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    8002.873 2.801 2.837 2.678
    4002.268 2.245 2.257 2.098
    2001.654 1.558 1.606 1.447
    1000.897 0.867 0.882 0.723
    500.581 0.562 0.572 0.413
    250.387 0.366 0.377 0.218
    12.50.298 0.284 0.291 0.132
    00.161 0.157 0.159 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)