Mouse 5-Hydroxytryptamine,5HT ELISA Kit
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中文名稱:小鼠5羥色胺(5-HT)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E08365m
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規(guī)格:96T/48T
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價(jià)格:¥3800/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:小鼠5羥色胺(5-HT)酶聯(lián)免疫試劑盒(CSB-E08365m)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的5HT含量。5-HT即血清素,是一種重要的神經(jīng)遞質(zhì)。它廣泛分布于中樞神經(jīng)系統(tǒng)與外周組織。在研究機(jī)制上,參與調(diào)節(jié)情緒、睡眠、食欲等多種生理功能,異常水平與抑郁癥等精神疾病相關(guān)。當(dāng)前圍繞其開展的研究,旨在開發(fā)相關(guān)藥物來治療各類身心疾病。試劑盒檢測(cè)范圍為250 pg/mL-4000 pg/ml,該產(chǎn)品適用于神經(jīng)科學(xué)研究中的遞質(zhì)動(dòng)態(tài)分析、藥理學(xué)實(shí)驗(yàn)的干預(yù)效果評(píng)估、行為學(xué)模型驗(yàn)證以及代謝相關(guān)疾病動(dòng)物模型的機(jī)制探索本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫:5HT
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:250 pg/mL-4000 pg/mL
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靈敏度:125 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Neuroscience
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測(cè)定原理:quantitative
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測(cè)定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse 5HT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 95 Range % 90-89 1:200 Average % 90 Range % 86-94 1:400 Average % 84 Range % 80-88 1:800 Average % 97 Range % 92-101 -
回收率:
The recovery of mouse 5HT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 87-99 EDTA plasma (n=4) 89 83-96 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average 4000 0.130 0.120 0.125 2000 0.243 0.241 0.242 1000 0.452 0.419 0.436 500 0.792 0.831 0.812 250 1.068 1.120 1.094 0 2.151 2.026 2.089 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Quercetin Alleviates Breast Cancer-Related Depression by Inhibiting Neutrophil Extracellular Traps via Inhibition of Sphingosine 1-Phosphate/Sphingosine 1-Phosphate Receptor Axis Q Zhu, Y Tao, Y Han, Y He, Y Fu, H Yang,Phytotherapy Research,2025
- Inhaling arsenic aggravates airway hyperreactivity by upregulating PNEC-sourced 5-HT in OVA-induced allergic asthma X Lu, ZX Tan, YX Yao, ZY Li, YY Zhu, QQ Yang,Ecotoxicology and Environmental Safety,2025
- Impact of Altered Gut Microbiota on Ketamine-Induced Conditioned Place Preference in Mice C Li, C Zhu, G Tu, Z Chen, Z Mo,Neuropsychiatric Disease and Treatment,2024
- The role of gut microbiota in anorexia induced by T-2 toxin T Huang,Ecotoxicology and environmental safety,2024
- Tryptophan metabolic pathway plays a key role in the stress-induced emotional eating Y Zhu,Current research in food science,2024
- Uncovering the effect and mechanism of Jiawei Xiaoyao Wan in treating breast cancer complicated with depression based on network pharmacology and experimental analysis Y Bai,Phytomedicine,2024
- Supplementation of Crataegi fructus alleviates functional dyspepsia and restores gut microbiota in mice L Hao,/,2024
- Kaempferol Improves Breast Cancer-Related Depression through the COX-2/PGE2 Pathway Q Zhu,IMR Press,2023
- Host miR-129-5p reverses effects of ginsenoside Rg1 on morphine reward possibly mediated by changes in B. vulgatus and serotonin metabolism in hippocampus C Li,Gut microbes,2023
- Asperosaponin VI Protects Against Bone Loss Due to Hindlimb Unloading in Skeletally Growing Mice Through Regulating Microbial Dysbiosis Altering the 5-HT Pathway YB Niu,Calcified tissue international,2023
- Theabrownin inhibits obesity and non-alcoholic fatty liver disease in mice via serotonin-related signaling pathways and gut-liver axis HY Li,Journal of advanced research,2023
- Anti-depressant-like effect of fermented Gastrodia elata Bl. by regulating monoamine levels and BDNF/NMDAR pathways in mice M Gao,Journal of ethnopharmacology,2022
- Bisphenol A impairs cognitive function and 5-HT metabolism in adult male mice by modulating the microbiota-gut-brain axis Yinhua Ni,Chemosphere,2021
- Saponin extracts from Gynostemma pentaphyllum (Thunb.) Makino display sedative-hypnotic and anxiolytic effects CY Shen,Industrial Crops and Products,2020
- Titanium dioxide nanoparticles via oral exposure leads to adverse disturbance of gut microecology and locomotor activity in adult mice Zhang S,Archives of Toxicology,2020
- Modulation of TGF-β/Smad and ERK signaling pathways mediates the anti-fibrotic effect of mirtazapine in mice El-Tanbouly DM.et al,Toxicol Appl Pharmacol,2017
- Physiological and behavioral responses in offspring mice following maternal exposure to sulfamonomethoxine during pregnancy. Zhang Q.et al,Neurosci Lett.,2016
產(chǎn)品評(píng)價(jià)
樣品類型:細(xì)胞培養(yǎng)上清液
樣品信息:小鼠
稀釋比:其他 1:2
產(chǎn)品評(píng)價(jià): 我用檢測(cè)PC12細(xì)胞上清液,標(biāo)準(zhǔn)曲線的OD值為y = -1.0317x + 2.6063,R2 = 0.9866 樣本的OD值為0.1084~0.2402 快速便捷,非常好用
By 廖老師
樣品類型:其他樣品處理方法 細(xì)胞勻漿液,用適量ripa裂解后取離心后上清做
樣品信息:小鼠
稀釋比:其他 1:20
產(chǎn)品評(píng)價(jià): CSB-E08365m檢測(cè)不同濃度的戊酸給給藥后的LLC培養(yǎng)上清液以及細(xì)胞勻漿液的5HT含量,標(biāo)準(zhǔn)曲線的OD值為:4000-0.08,2000-0.15,1000-0.17,500-0.43,250-0.87,0-1.01,樣品OD值為0.19~0.67,陰性對(duì)照是0.99左右,產(chǎn)品快速便捷,操作簡(jiǎn)單,重復(fù)性較好,但是貌似沒有對(duì)細(xì)胞樣品的處理指南。陰性對(duì)照是水(0.9900,0.9142,0.9521),樣品則為0.19~0.67;標(biāo)曲的0濃度吸光值為(1.0943,1.0101)。
By 廖老師
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