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Mouse Activin A,ACV-A ELISA Kit

  • 中文名稱:
    小鼠活化素A(ACV-A)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E04502m
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3800/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    小鼠活化素A(ACV-A)酶聯(lián)免疫試劑盒(CSB-E04502m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的ACV-A含量。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL,靈敏度為3.9 pg/mL。適用于小鼠模型在基礎(chǔ)研究中的多場景應(yīng)用,例如探究生殖生物學(xué)機制、炎癥性疾病模型中的分子調(diào)控、組織再生研究或藥物干預(yù)下ACV - A的動態(tài)變化分析,助力于發(fā)育生物學(xué)、免疫學(xué)及病理機制等領(lǐng)域的深入探索。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    ACV-A
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    15.6 pg/mL-1000 pg/mL
  • 靈敏度:
    3.9 pg/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

     

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(pg/ml)

    122.000

    132.198

    122.651

    123.043

    120.448

    120.448

    SD

    0.055

    0.064

    0.063

    0.071

    0.068

    0.074

    CV(%)

    5.56

    6.01

    6.34

    7.12

    6.96

    7.58

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ACV-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    95

    Range %

    86-105

    1:2

    Average %

    96

    Range %

    91-100

    1:4

    Average %

    93

    Range %

    90-99

    1:8

    Average %

    96

    Range %

    89-100

  • 回收率:

    The recovery of mouse ACV-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    89-99

    EDTA plasma (n=4)

    97

    92-100

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    1000

    2.249

    2.268

    2.259

    2.175

    500

    2.035

    2.055

    2.045

    1.961

    250

    1.601

    1.586

    1.594

    1.510

    125

    0.954

    0.972

    0.963

    0.879

    62.5

    0.534

    0.521

    0.528

    0.444

    31.2

    0.272

    0.283

    0.278

    0.194

    15.6

    0.184

    0.190

    0.187

    0.103

    0

    0.083

    0.085

    0.084

     

  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 最新研究進展:
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        α2-MG與腫瘤的關(guān)聯(lián)性也成為研究熱點。研究人員發(fā)現(xiàn),α2-MG在某些腫瘤中的表達水平顯著升高,與腫瘤的侵襲和預(yù)后密切相關(guān)。此外,α2-MG還能夠影響腫瘤細胞的增殖和轉(zhuǎn)移,并參與腫瘤微環(huán)境的調(diào)節(jié)。
        α2-MG在神經(jīng)系統(tǒng)中的作用也備受關(guān)注。研究發(fā)現(xiàn),α2-MG可以抑制神經(jīng)元的凋亡和炎癥反應(yīng),從而保護神經(jīng)系統(tǒng)免受損傷。此外,α2-MG還可以影響神經(jīng)元的突觸可塑性,從而調(diào)節(jié)神經(jīng)功能。
        α2-MG在腎臟疾病中的作用也得到了研究。研究發(fā)現(xiàn),α2-MG在腎臟疾病中的水平可以反映腎臟功能的損傷程度,并且α2-MG的降解和清除與腎臟疾病的進展密切相關(guān)。