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  4. Mouse Alanine Aminotransferase(ALT/GPT)ELISA Kit

Mouse Alanine Aminotransferase(ALT/GPT)ELISA Kit

  • 中文名稱：
    小鼠丙氨酸轉(zhuǎn)氨酶/谷丙轉(zhuǎn)氨酶(ALT/GPT)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E16539m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠丙氨酸轉(zhuǎn)氨酶/谷丙轉(zhuǎn)氨酶(ALT/GPT)酶聯(lián)免疫試劑盒（CSB-E16539m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、細(xì)胞裂解物樣本中的ALT/GPT含量。ALT/GPT即谷丙轉(zhuǎn)氨酶，主要存在于肝細(xì)胞內(nèi)。肝細(xì)胞受損時(shí)其釋放入血，致血清ALT/GPT水平升高，是反映肝細(xì)胞損傷的敏感指標(biāo)。臨床常通過(guò)檢測(cè)其活性輔助診斷肝臟疾病，如肝炎、肝硬化等，研究也圍繞其在疾病中的變化機(jī)制展開(kāi)。試劑盒檢測(cè)范圍為0.156 mU/mL-10 mU/mL，廣泛應(yīng)用于肝損傷模型評(píng)估、藥物肝毒性研究、代謝性疾病機(jī)制探索等科研領(lǐng)域，特別適用于體外培養(yǎng)肝細(xì)胞或動(dòng)物模型中肝臟相關(guān)病理生理變化的動(dòng)態(tài)監(jiān)測(cè)，為肝臟功能研究提供可靠的實(shí)驗(yàn)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    Gpt ELISA kit; Gpt1Alanine aminotransferase 1 ELISA kit; ALT1 ELISA kit; EC 2.6.1.2 ELISA kit; Glutamate pyruvate transaminase 1 ELISA kit; GPT 1 ELISA kit; Glutamic--alanine transaminase 1 ELISA kit; Glutamic--pyruvic transaminase 1 ELISA kit
  • 縮寫：
    ALT/GPT
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, cell lysates
  • 檢測(cè)范圍：
    0.156 mU/mL-10 mU/mL
  • 靈敏度：
    0.039 mU/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ALT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %88
    Range %83-95
    1:2Average %97
    Range %92-101
    1:4Average %106
    Range %98-113
    1:8Average %94
    Range %87-97
  • 回收率：
    The recovery of mouse ALT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9288-97
    EDTA plasma (n=4)10295-108
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/mlOD1OD2AverageCorrected
    102.645 2.566 2.606 2.421
    52.015 1.855 1.935 1.750
    2.51.194 1.155 1.175 0.990
    1.250.777 0.796 0.787 0.602
    0.6250.492 0.461 0.477 0.292
    0.3120.335 0.344 0.340 0.155
    0.1560.278 0.288 0.283 0.098
    00.191 0.178 0.185 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
  • 基因功能參考文獻(xiàn)：
    1. Dexamethasone induces a metabolic adaptive ALT isoenzyme response in livers of C57/BL6 mice. PMID: 22609950
    2. PKCdelta activation and protein levels are elevated in an animal model of steatohepatitis, which was recapitulated in a cell model, supporting the conclusion that PKCdelta plays a role in ALT release, the ER stress signal, and cell death PMID: 20971848
    3. In experiments where mice were provided ethanol chronically in drinking water, where stress is minimal, higher levels of ALT were noted in animals in the ethanol group as compared with animals in the control group. PMID: 15652761
  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class-I pyridoxal-phosphate-dependent aminotransferase family, Alanine aminotransferase subfamily
  • 組織特異性：
    Mainly expressed in liver, intestine, colon and white adipose tissue.
  • 數(shù)據(jù)庫(kù)鏈接：