Mouse Anti-Smooth Muscle Antibody,ASMA ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

小鼠抗平滑肌抗體(ASMA)酶聯(lián)免疫試劑盒（CSB-E08795m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ASM Ab含量。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，該產(chǎn)品可為研究者提供小鼠模型中ASMA水平的動態(tài)監(jiān)測，支持自身免疫性疾病機(jī)制探索、藥物干預(yù)效果評估及病理模型構(gòu)建等科研需求，尤其適用于肝臟、血管相關(guān)疾病動物實(shí)驗(yàn)的樣本分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

ASM Ab
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ASMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	87-102
1:2	Average %	98
	Range %	90-101
1:4	Average %	89
	Range %	84-93
1:8	Average %	107
	Range %	98-111

回收率：

The recovery of mouse ASMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	81-94
EDTA plasma (n=4)	114	102-117

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.