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  4. Mouse Apolipoprotein C-III(APOC3) ELISA kit

Mouse Apolipoprotein C-III(APOC3) ELISA kit

  • 中文名稱：
    小鼠載脂蛋白C-III(APOC3)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL001933MO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠載脂蛋白C-III(APOC3)酶聯(lián)免疫試劑盒（CSB-EL001933MO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的APOC3含量。APOC3是一種重要的靶點。它在脂質(zhì)代謝中發(fā)揮關(guān)鍵作用，其異常表達(dá)與高脂血癥等疾病密切相關(guān)。研究機制主要集中于探究其調(diào)控血脂的具體分子通路，旨在通過干預(yù)APOC3來調(diào)節(jié)血脂水平，為相關(guān)心血管疾病的防治提供新策略。試劑盒檢測范圍為18.75 ng/mL-1200 ng/mL，該產(chǎn)品適用于高脂血癥、糖尿病或動脈粥樣硬化等代謝性疾病小鼠模型中APOC3的動態(tài)監(jiān)測，也可用于探究肝臟、脂肪組織等器官中APOC3的表達(dá)分布及調(diào)控機制。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Apoc3Apolipoprotein C-III ELISA kit; Apo-CIII ELISA kit; ApoC-III ELISA kit; Apolipoprotein C3 ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    18.75 ng/mL-1200 ng/mL
  • 靈敏度：
    9.375 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse APOC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 94  
    Range % 90-98  
    1:200 Average % 91  
    Range % 88-95  
    1:400 Average % 83  
    Range % 80-87  
    1:800 Average % 88  
    Range % 84-92  
  • 回收率：
    The recovery of mouse APOC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 92-103  
    EDTA plasma (n=4) 86 82-92  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    1200 0.087 0.089 0.088    
    600 0.188 0.191 0.190    
    300 0.382 0.378 0.380    
    150 0.653 0.645 0.649    
    75 1.131 1.146 1.139    
    37.5 1.516 1.527 1.522    
    18.75 1.819 1.921 1.870    
    0 2.369 2.265 2.317    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma. Plays a multifaceted role in triglyceride homeostasis. Intracellularly, promotes hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion; extracellularly, attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs). Impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by remnant receptors. Formed of several curved helices connected via semiflexible hinges, so that it can wrap tightly around the curved micelle surface and easily adapt to the different diameters of its natural binding partners.
  • 基因功能參考文獻：
    1. Both triglyceride-rich lipoproteins (TRLs) and ApoCIII contribute to the progression of atherosclerosis, and the modulation of TRLs and ApoCIII may represent a novel therapeutic approach against hypertriglyceridemia induced atherosclerosis. PMID: 30223835
    2. C3(QK) variant is a gain-of-function mutation that can stimulate VLDL1 production, through enhanced DNL PMID: 28887372
    3. intestinal apoC-III overexpression results in the secretion of smaller, less dense chylomicron particles along with reduced triacylglycerol secretion from the intestine PMID: 28159868
    4. ApoC-III inhibits turnover of TG-rich lipoproteins primarily through a hepatic clearance mechanism mediated by the LDLR/LRP1 axis PMID: 27400128
    5. These data strongly suggest that intestinal apoC-III is not a FoxO1 target and support the idea that apoC-III is not regulated coordinately with hepatic apoC-III, and establishes another key aspect of apoC-III that is unique in the intestine from the liver. PMID: 28739253
    6. APOC3, whose dysregulation is liable for hypertriglyceridemia, is not a predisposing factor for linking overnutrition to NAFLD in obesity PMID: 28115523
    7. Severe hypertriglyceridaemia resulting from ApoCIII overexpression promotes restenosis and atherosclerosis PMID: 26160324
    8. Under conditions of islet insulin resistance, locally produced apoCIII is an important diabetogenic factor involved in impairment of beta-cell function. PMID: 25941406
    9. decreased ApoAI synthesis might be accounted for the lower plasma HDL level in ApoCIII transgenic mice PMID: 25969427
    10. ApoCIII hyperactivates beta cell CaV1 channels through SR-BI/beta1 integrin-dependent coactivation of PKA and Src. PMID: 23949443
    11. Increased plasma APOC3 concentrations predispose mice to diet-induced nonalcoholic fatty liver and hepatic insulin resistance. PMID: 21793029
    12. Glucose induces apoCIII transcription, which may represent a mechanism linking hyperglycemia, hypertriglyceridemia, and cardiovascular disease in type 2 diabetes. PMID: 21183731
    13. PGC-1beta regulates plasma triglyceride metabolism through stimulating apolipoprotein C3 (APOC3) expression and elevating APOC3 levels in circulation PMID: 20889132
    14. The apoC-III metabolism may contribute to dyslipidemia in CKD, and this requires further investigation. PMID: 19542564
    15. Association between SstI polymorphism of the gene, glucose intolerance and cardiovascular risk in renal transplant recipients PMID: 11959336
    16. ApoB lipoproteins that contain apoCIII increase THP-1 cell adhesion to ECs via PKCalpha and RhoA-mediated beta1-integrin activation. PMID: 16461842
    17. oxidized fatty acids may act through an APOA5/APOClll mechanism that contributes to lowering of TG levels other than PPAR* induction PMID: 18243209
    18. the apoCIII enhancer contributes to the maintenance of an active chromatin subdomain of the apoAI/CIII/AIV genes, but not apoAV PMID: 18678879
    19. These results suggest that apoC-III may play a specific role in lipid storage and mobilization in adipocytes, non-lipoprotein-secreting cells, and indicate the functional role of RXRalpha during adipocyte differentiation. PMID: 19121312

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Apolipoprotein C3 family
  • 數(shù)據(jù)庫鏈接：