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  4. Mouse Aromatase(ARO) ELISA Kit

Mouse Aromatase(ARO) ELISA Kit

  • 中文名稱：
    小鼠芳香酶酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E14312m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠芳香酶酶聯(lián)免疫試劑盒（CSB-E14312m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CYP19A1含量。CYP19A1又稱芳香化酶，是催化雄激素轉(zhuǎn)化為雌激素的限速酶。其作為重要藥物靶點(diǎn)，在乳腺癌等激素依賴性疾病中高度表達(dá)，與腫瘤生長、增殖密切相關(guān)。研究機(jī)制主要圍繞抑制其活性，減少雌激素合成，以此達(dá)到治療疾病的目的。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，適用于研究芳香酶在激素代謝調(diào)控中的作用，廣泛參與生殖系統(tǒng)發(fā)育、內(nèi)分泌平衡及性激素依賴性疾病的分子機(jī)制研究，可為生殖生物學(xué)、腫瘤微環(huán)境分析及內(nèi)分泌疾病模型構(gòu)建提供可靠數(shù)據(jù)支持，例如用于卵巢功能評估、激素代謝異常研究或藥物干預(yù)下酶活性變化的動(dòng)態(tài)監(jiān)測本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Cyp19a1 ELISA kit; Arom ELISA kit; Cyp19Aromatase ELISA kit; EC 1.14.14.14 ELISA kit; CYPXIX ELISA kit; Cytochrome P-450AROM ELISA kit; Cytochrome P450 19A1 ELISA kit; Estrogen synthase ELISA kit
  • 縮寫：
    CYP19A1
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ARO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 100  
    Range % 95-106  
    1:2 Average % 89  
    Range % 84-93  
    1:4 Average % 93  
    Range % 89-96  
    1:8 Average % 98  
    Range % 93-104  
  • 回收率：
    The recovery of mouse ARO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-99  
    EDTA plasma (n=4) 92 86-98  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    400 2.258 2.112 2.185 2.009  
    200 1.644 1.695 1.670 1.494  
    100 1.028 1.112 1.070 0.894  
    50 0.588 0.604 0.596 0.420  
    25 0.334 0.358 0.346 0.170  
    12.5 0.249 0.257 0.253 0.077  
    6.25 0.214 0.226 0.220 0.044  
    0 0.181 0.171 0.176 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3,17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively. Catalyzes three successive oxidations of C19 androgens: two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid. Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen. Also displays 2-hydroxylase activity toward estrone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
  • 基因功能參考文獻(xiàn)：
    1. This study demonstrated that Aromatase expression in spinal cord and medulla in sex difference. PMID: 28649695
    2. These studies suggest an association between adiposity, aromatase estrogenic capacity and atrial arrhythmogenicity. PMID: 28822806
    3. Increased adipose tissue aromatase activity reduces adipose tissue inflammation and improves insulin sensitivity in male mice. PMID: 28655716
    4. Aromatase-knockout (ArKO) mice cannot synthesize estrogens in vivo. Male ArKO mice developed hepatic steatosis accompanied by high testosterone levels. PMID: 28341248
    5. results show that the Aha1-Hsp90-PKM2/HIF-1alpha axis mediates the induction of aromatase in Li-Fraumeni Syndrome. PMID: 27467582
    6. aromatase could modulate pituitary LH-positive cells in males through local estradiol synthesis. PMID: 27160809
    7. Findings indicate that sex chromosome factors determine sex differences in aromatase expression in the stria terminalis and in the anterior amygdaloid area of mouse embryos. PMID: 26231585
    8. Aromatase plays an important role in the survival of metastatic ERalpha breast cancer cells by suppressing anoikis. PMID: 25837259
    9. aromatase may play a key role in the pathogenesis of Sjogren syndrome-like lesions by controlling the target organ and adipose tissue-associated macrophage; increased MPC1 protein expression has a role in adiposity PMID: 25447050
    10. The protective effect of female gender on abdominal aortic aneurysms is due to estrogen synthesis and requires the presence of both ovarian and extragonadal/peripheral aromatase. PMID: 24582702
    11. Male Aromatase knockout mice develop hepatic glucose intolerance. PMID: 24520329
    12. Cerebrovascular endothelial aromatase plays an important and sexually dimorphic role in cerebrovascular function and that aromatase inhibitors in clinical use may have cardiovascular consequences in both males and females. PMID: 24508640
    13. CYP19A1 gene is driven by promoters PII, I.4, and I.3 in the seminal vesicles, and by promoters PII and I.4 in the urinary bladder. PMID: 23239821
    14. Lhx2 is involved in the transcriptional regulation of aromatase in the rodent brain. PMID: 22734700
    15. Glucocorticoids alter aromatase expression in the hypothalamus and might regulate gonadotropin pulses and the menstrual cycle. PMID: 22705581
    16. Aromatase-deficient mice displayed increased cortical neuronal density and occasional cortical heterotopias PMID: 22426781
    17. Data show that the human aromatase gene can be expressed via its native promoters in a wide variety of mouse tissues and in a distribution pattern nearly identical to that of humans. PMID: 22508516
    18. expression level of estrogen receptors and aromatase in testis during aging PMID: 22366072
    19. investigation of role of aromatase in disease and inflammatory processes: Characterization of aromatase and apolipoprotein E double knockout mice as model of metabolic syndrome X. PMID: 22185842
    20. Impact of ovarian sex steroids on ovulation and ovulatory gene induction in aromatase-null mice. PMID: 22147013
    21. in the acute ischemia/reperfusion setting, more robust functional recovery, reduced cellular injury, and modified cardiomyocyte Ca(2+) handling was observe in aromatase-deficient hearts PMID: 22028441
    22. An organizational effect of estradiol during a critical period of development on the expression of biological rhythms. PMID: 21816154
    23. Mechanisms involved in amphetamine-induced c-fos expression are altered in aromatase knockout mice. PMID: 21093158
    24. Obesity is associated with inflammation and elevated aromatase expression in the mouse mammary gland PMID: 21372033
    25. The sexual differentiation of kisspeptin neuronal number was lost in aromatase knockout mice PMID: 19945459
    26. The study provides the first in vivo evidence linking the LH receptor, the EGFR, and ERK1/2 as sequential components of a pathway that regulates ovarian Cyp19a1 expression. PMID: 20093417
    27. Liver mitochondria isolated from ArKO mice displayed increased activity of the mitochondrial respiratory complex IV and were less prone to undergo cyclosporin A-sensitive mitochondrial permeability transition induced by calcium loading. PMID: 20194728
    28. FXR is able to compete with steroidogenic factor 1 in binding to a common sequence present in the aromatase promoter region interfering negatively with its activity. PMID: 20026603
    29. Data describe the effects of genistein and three synthetic estrogen receptor alpha/beta agonists in aromatase knockout mice by two routes of administration. PMID: 19686804
    30. Aromatase-deficient (ArKO) mice accumulate excess adipose tissue. PMID: 11850201
    31. Alternations in hepatic expression of fatty-acid metabolizing enzymes in ArKO mice and their reversal by the treatment with 17beta-estradiol or a peroxisome proliferator. PMID: 11850202
    32. Aromatase overexpression transgenic mice model: cell type specific expression and use of letrozole to abrogate mammary hyperplasia without affecting normal physiology. PMID: 11850204
    33. Bone phenotype of the aromatase deficient mouse. PMID: 11850207
    34. The ovarian phenotype of the aromatase knockout (ArKO) mouse. PMID: 11850223
    35. Characterization and purification of a protein binding to the cis-acting element for brain-specific exon 1 of the mouse aromatase gene. PMID: 11850232
    36. REVIEW: effects of depletion of endogenous estrogens in the aromatase deficient mouse (ArKO) with respect to spermiogenesis, development of obesity, and development of osteoporosis PMID: 12160996
    37. Inhibition of p-450 aromatase prevents feminisation and induces protection during cysticercosis PMID: 12350373
    38. The mouse Cyp19 gene includes three different promoters that specifically direct expression in ovary, testis, and brain. PMID: 12604651
    39. Induction of Fos in the accessory olfactory system by male odors persists in female mice with a null mutation of the aromatase (cyp19) gene. PMID: 12725902
    40. Forebrain Fos responses to reproductively related chemosensory cues in aromatase knockout mice. PMID: 12754080
    41. In male, but not female, aromatase knockout mice, there was an age-related reduction of prepulse inhibition of acoustic startle. PMID: 12884966
    42. aromatase knockout females presented with elevated serum cholesterol; conversely, aromatase knockout males had elevated hepatic cholesterol levels. PMID: 12933663
    43. These findings indicate that the biosynthetic enzyme P450 aromatase is key to endogenous neuroprotection in females and suggest that enhancing local, nongonadal estrogen formation could have therapeutic implications in cerebral ischemic neuropathology PMID: 14507969
    44. Review confirms the findings of aromatase neuroprotective effect in reactive astrocytes in vitro through use of aromatase knockout mice, which show enhanced hippocampal neurodegeneration in response to neurotoxins. PMID: 14993062
    45. aromatase activity is critical for normal autonomic control of the heart and, hence, for reducing the deleterious effects of high BP variability. PMID: 15178650
    46. Oestrogen-deficient female aromatase knockout (ArKO) mice exhibit depressive-like symptomatology. PMID: 15245494
    47. PGF(2alpha) decreases luteal P450arom mRNA levels at the end of pregnancy in rodents by inhibiting cyp19 expression. PMID: 15271885
    48. The aromatase immunostaining was found in cryptorchid-KE Leydig cells. PMID: 15823796
    49. Aromatase transgenic mice show increased levels of the estrogen receptor alpha and beta (ERalpha, ERbeta) suggesting that this receptor may play an important role in the initiation of estrogen-mediated mammary hyperplasia observed in these mice. PMID: 15955696
    50. p450 aromatase may have a role in male infertility associated with chronic inflammation in the testis PMID: 16306085

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  • 亞細(xì)胞定位：
    Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein.
  • 蛋白家族：
    Cytochrome P450 family
  • 數(shù)據(jù)庫鏈接：