Mouse Biglycan(BGN) ELISA kit
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中文名稱(chēng):小鼠雙糖鏈蛋白聚糖(BGN)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-EL002683MO
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:小鼠雙糖鏈蛋白聚糖(BGN)酶聯(lián)免疫試劑盒(CSB-EL002683MO)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的BGN含量。BGN作為靶點(diǎn),其它在特定生理和病理過(guò)程中發(fā)揮作用。研究機(jī)制上,圍繞其在細(xì)胞信號(hào)傳導(dǎo)、分子相互作用等層面展開(kāi)探究,旨在明確其功能及與疾病的關(guān)聯(lián),為相關(guān)疾病的診斷、治療方案制定及新藥研發(fā)提供依據(jù)。試劑盒檢測(cè)范圍為0.625 ng/mL-40 ng/mL,科研人員可通過(guò)該產(chǎn)品探究BGN在組織纖維化模型(如肝纖維化、肺纖維化)、腫瘤發(fā)生發(fā)展機(jī)制、炎癥性疾病中的動(dòng)態(tài)變化,或用于評(píng)估基因編輯動(dòng)物模型中BGN的表達(dá)調(diào)控效果,為細(xì)胞外基質(zhì)相關(guān)分子機(jī)制研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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別名:Bgn ELISA Kit; Biglycan ELISA Kit; Bone/cartilage proteoglycan I ELISA Kit; PG-S1 ELISA Kit
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縮寫(xiě):
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類(lèi)型:serum, plasma, tissue homogenates
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檢測(cè)范圍:0.625 ng/mL-40 ng/mL
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靈敏度:0.156 ng/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Signal Transduction
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse BGN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 102 Range % 99-103 1:2 Average % 95 Range % 91-99 1:4 Average % 88 Range % 84-92 1:8 Average % 99 Range % 95-103 -
回收率:
The recovery of mouse BGN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 104 100-108 EDTA plasma (n=4) 93 89-97 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 40 2.378 2.496 2.437 2.319 20 1.863 1.965 1.914 1.796 10 1.432 1.467 1.450 1.332 5 1.025 1.090 1.058 0.940 2.5 0.666 0.644 0.655 0.537 1.25 0.437 0.470 0.454 0.336 0.625 0.269 0.288 0.279 0.161 0 0.116 0.119 0.118 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Inhibition of bone morphogenetic protein 4 alleviates angiotensin II-induced abdominal aortic aneurysm by reducing inflammation and endothelial-mesenchymal transition Y Weng, X Wang, Y Tang, C Du, X Li, K Zhu, Y Bao,Atherosclerosis,2025
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:May be involved in collagen fiber assembly.
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基因功能參考文獻(xiàn):
- Knockout results provide evidence that decorin and biglycan are necessary for maintaining collagen fibril structure, fiber realignment, and mechanical properties of mature tendons. PMID: 28882761
- A novel biological pathway has been discovered of soluble biglycan inducing HIF-2alpha protein stabilization and Epo production presumably in an oxygen-independent manner, ultimately giving rise to secondary polycythemia. PMID: 27600268
- Results show that Bgn plays a role in the process of angiogenesis during fracture healing, and that effect appears to be partially mediated through endostatin suppression. PMID: 27072616
- Asporin deficiency changes skin glycosaminoglycan composition, and decorin and biglycan content, which may explain the changes in skin mechanical properties. PMID: 28859141
- Biglycan plays a protective role during the progression of atherosclerosis in ApoE-deficient mice by inhibiting thrombin generation. PMID: 27034473
- These genes were concordantly induced by TAC in WT but not in biglycan KO mice. CONCLUSIONS: Left ventricular pressure overload induces biglycan expression in cardiac fibroblasts. Ablation of biglycan improves cardiac function and attenuates left ventricular hypertrophy and fibrosis after long-term pressure overload. PMID: 27789290
- Importance of biglycan and decorin as targets for the manipulation of fetal membrane extracellular matrix stability in the context of inflammation. PMID: 25914258
- Biglycan deficiency leads to loosely packed aortic collagen fibers, increased susceptibility of aortic elastin fibers to angII-induced stress, and up-regulation of vascular perlecan content. PMID: 25093698
- Biglycan signaling supported fetal membrane remodeling during early gestation in the absence of concomitant changes in TGFbeta levels. PMID: 24373743
- Lumican and biglycan influence corneal keratocyte lamellipodia organization and are critical in the regulation of stromal collagen fibrillogenesis. PMID: 24447998
- Biglycan-triggered TLR-2- and TLR-4-signaling exacerbates the pathophysiology of ischemic acute kidney injury. PMID: 24480070
- De novo expression of circulating biglycan evokes an innate inflammatory tissue response via MyD88/TRIF pathways. PMID: 24361484
- Early stage patellar tendon healing was inferior in biglycan-null and decorin-null mice as compared to wild type. PMID: 24157578
- This suggests that biglycan and decorin may have sequential roles in the tendon response to injury. PMID: 24072490
- Mast cell chymase degrades the alarmins heat shock protein 70, biglycan, HMGB1, and interleukin-33 (IL-33) and limits danger-induced inflammation. PMID: 24257755
- extracellular matrix biglycan mediates breast cancer normalization induced by embryonic mesenchyme PMID: 23817524
- Biglycan plays a role in tendon viscoelasticity that cannot be completely explained by its role in collagen fibrillogenesis. PMID: 23592048
- Biglycan deletion alters adiponectin expression in murine adipose tissue and 3T3-L1 adipocytes. PMID: 23189205
- The glycosaminoglycans chains of biglycan (BGN) promote BGN-assisted BMP-4 function. PMID: 22895561
- Bgn inhibits the major properties of HER-2/neu-transformed cells, which is inversely modulated by the PKC signaling cascade. PMID: 22582394
- Increased messenger RNA (mRNA) expression of extracellular matrix genes BGN and COL1A1 was observed in the mouse epididymal adipose tissue after high-fat diet. PMID: 21775118
- Biglycan/decorin mixed double knockout mouse is a model of dystocia and delayed labor onset. PMID: 22253749
- Extracellular matrix protein biglycan plays a novel role in regulating synapse stability. PMID: 22396407
- Use of a transplant system and a fracture healing model revealed that expression of Wnt-induced secreted protein 1 was decreased in bone formed by biglycan-deficient cells, further suggesting reduced Wnt signaling in vivo. PMID: 21969569
- Biglycan is expressed/developmentally regulated in placenta and fetal membranes; data from mutant mouse strains suggest that both biglycan and decorin contribute to gestational success (i.e., prevent premature birth). PMID: 21502335
- critical for temporomandibular joint subchondral bone integrity PMID: 21917603
- Biglycan and decorin reduced the proliferation of pre-adipocytes, partly by induction of apoptosis. Furthermore, the anti-proliferative capabilities of decorin and biglycan were nullified with removal of GAG side-chains. PMID: 21702857
- bgn deficiency promotes myofibroblast differentiation and proliferation in vitro and in vivo likely due to increased responses to TGF-beta and SMAD2 signaling PMID: 21454527
- Biglycan and fibromodulin are novel key players in regulating chondrogenesis and extracellular matrix turnover during temoporomandibular joint osteoarthritis pathology. PMID: 20035055
- Biglycan deficiency significantly accelerated disc degeneration in mice. PMID: 19940720
- Beta1 integrin and htis protein's binding sites are localized to different laminin-G-domain-like (LG) modules within the laminin alpha5 chain G domain. PMID: 12519075
- decorin and biglycan play distinct roles in palatogenesis, and decorin was more actively involved in the process of secondary palate formation than biglycan. PMID: 12666199
- biglycan deficient mice have diminished capacity to produce marrow stromal cells, the bone cell precursors, and this deficiency increases with age PMID: 12975603
- Deficiency protects against increased trabecular bone turnover and bone loss in response to estrogen depletion. PMID: 14672350
- bgn is required for retaining an appropriate microenvironment for the maturation of osteoblastic stem cells PMID: 15173106
- In addition to the imbalance between differentiation and proliferation, there was a differential decrease in secretory leukocyte protease inhibitor (slpi) in bgn deficient osteoblasts treated with 1,25-(OH)(2)D(3) PMID: 16364709
- biglycan is important for the maintenance of muscle cell integrity and plays a direct role in regulating the expression and sarcolemmal localization of the intracellular signaling proteins dystrobrevin-1 and -2, alpha- and beta1-syntrophin and nNOS PMID: 16807372
- biglycan has a particularly important function during muscle and connective tissue development PMID: 16810681
- Biglycan is a ligand for two members of the sarcoglycan complex and regulates their expression at discrete developmental ages. PMID: 16883602
- Biglycan is a positive modulator of BMP-2 induced osteoblast differentiation PMID: 17120779
- At embryonic day 18.5, alizarin red/alcian blue staining showed that the Bgn/Dcn double deficient mice had hypomineralization of the frontal and parietal craniofacial bones. PMID: 17188951
- death of biglycan-deficient mice from aortic rupture implicates biglycan as essential for the structural and functional integrity of the aortic wall and implicates biglycan gene defects in the pathogenesis of aortic dissection and rupture PMID: 17502576
- These preliminary data suggest that WISP-1 and BGN may functionally interact and control each other's activity, thus regulating the differentiation and proliferation of osteogenic cells. PMID: 18701807
- Biglycan overexpression did not significantly affect the amelogenin expression in incisor and molar teeth in 3-day postnatal transgenic mice. PMID: 18727043
- decorin has a primary role in regulating fibril assembly, a function that can be fine-tuned by biglycan during early development. PMID: 19136671
- Findings indicate that normal expression of small leucine rich proteoglycans, such as biglycan and decorin, plays an important role in the highly orchestrated process of dentin mineralization. PMID: 19379665
- By signaling through TLR2/4, biglycan stimulates the expression of NLRP3 and pro-IL-1beta mRNA. PMID: 19605353
- Binds to the carboxyl-terminal third of the alpha-dystroglycan core polypeptide. This interaction requires the chondroitin sulfate side chains of biglycan. PMID: 10684260
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亞細(xì)胞定位:Secreted, extracellular space, extracellular matrix.
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蛋白家族:Small leucine-rich proteoglycan (SLRP) family, SLRP class I subfamily
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組織特異性:Found in several connective tissues, especially in articular cartilages.
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