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  4. Mouse C-C motif chemokine 25(CCL25) ELISA kit

Mouse C-C motif chemokine 25(CCL25) ELISA kit

  • 中文名稱：
    小鼠C-C趨化因子25（CCL25）酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL004789MO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠C-C趨化因子25（CCL25）酶聯(lián)免疫試劑盒（CSB-EL004789MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CCL25含量。CCL25，又稱胸腺表達(dá)趨化因子TECK，是一種CC趨化因子家族的小細(xì)胞因子，主要表達(dá)于胸腺和腸上皮。CCL25通過與其受體CCR9結(jié)合，參與T細(xì)胞的發(fā)育和細(xì)胞遷移，以及多種炎癥性疾病，并促進(jìn)炎癥反應(yīng)。其相互作用還可通過Akt/蛋白激酶B激活抑制細(xì)胞凋亡，支持T細(xì)胞存活。試劑盒檢測范圍為78 pg/ml- 5000 pg/ml，靈敏度為19.5 pg/ml。適用于多種科研場景，例如探索腸道炎癥性疾病模型（如結(jié)腸炎）中趨化因子的表達(dá)調(diào)控機(jī)制、評估免疫細(xì)胞遷移相關(guān)信號通路的功能研究，或用于藥物開發(fā)過程中靶向CCL25/CCR9軸的治療性化合物體外效果驗(yàn)證。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Ccl25 ELISA Kit; Scya25 ELISA Kit; Teck ELISA Kit; C-C motif chemokine 25 ELISA Kit; Chemokine TECK ELISA Kit; Small-inducible cytokine A25 ELISA Kit; Thymus-expressed chemokine ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    78 pg/ml - 5000 pg/ml
  • 靈敏度：
    19.5 pg/ml
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CCL25 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    101

    Range %

    98-105

    1:2

    Average %

    99

    Range %

    96-103

    1:4

    Average %

    89

    Range %

    85-94

    1:8

    Average %

    97

    Range %

    92-103

  • 回收率：

    The recovery of mouse CCL25 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    91-98

    EDTA plasma (n=4)

    88

    83-92

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    5000

    2.357

    2.366

    2.362

    2.231

    2500

    1.825

    1.812

    1.819

    1.688

    1250

    1.260

    1.366

    1.313

    1.182

    625

    0.751

    0.745

    0.748

    0.617

    312

    0.478

    0.498

    0.488

    0.357

    156

    0.313

    0.322

    0.318

    0.187

    78

    0.217

    0.221

    0.219

    0.088

    0

    0.128

    0.133

    0.131

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
  • 基因功能參考文獻(xiàn)：
    1. these results showed a site- and cell-specific expressions of Ccl25 and its receptor Ccr9 within both maternal and embryonic compartments of implantation sites and suggested a role in inducing specific Ccr9+ leukocytes at the maternal-fetal interface during the initial steps of embryo implantation in the mouse model PMID: 29154408
    2. MicroRNA-205 maintains T cell development following stress by regulating Foxn1 and its two regulated targets, stem cell factor and ccl25, following stress. PMID: 27646003
    3. CCR9 and CCL25 expressions are induced in the early stages of airway inflammation and they have an important role modulating eosinophils and lymphocytes recruitment at the first stages of inflammatory process PMID: 27795621
    4. TECK was shown to be expressed by osteoblasts, and its receptor, CCR9, by osteoclast precursors. TECK increased P. gingivalis LPS-induced osteoclast numbers in an in vitro osteoclast formation assay using osteoclast precursors. T PMID: 26921718
    5. CCR9/CCL25 is involved in acute skin transplantation rejection and anti-CCL25 strategies might be useful in preventing acute rejection. PMID: 23456208
    6. Accumulated CD11b(+) macrophages are critical for activating hepatic stellate cells through the CCR9/CCL25 axis and therefore promote liver fibrosis. PMID: 23460364
    7. The results revealed that during an allergic reaction, CCL25 drives IL-17 gammadelta T-cell mobilization to inflamed tissue via alpha4beta7 integrin and modulates IL-17 levels. PMID: 22539297
    8. CCL25/CCR9 interactions regulate inflammatory immune responses in the large intestinal mucosa by balancing different subsets of dendritic cells. PMID: 21283540
    9. Characterization of mouse CCX-CKR, a receptor for the lymphocyte-attracting chemokines TECK/mCCL25, SLC/mCCL21 and MIP-3beta/mCCL19: comparison to human CCX-CKR. (CCX-CKR) PMID: 11981810
    10. in vivo neutralization of the CCR9 ligand, CCL25, reduced the ability of activated CCR9(+) CD8alphabeta(+) lymphocytes cells to populate the small-intestinal epithelium PMID: 12393847
    11. role of CCL25 in the generation of the small-intestinal CD8alpha alpha(+)CD3(+) intraepithelial lymphocyte compartment PMID: 12442331
    12. chemokine (C-C motif) ligand 25 may thus play an important role in the adherence of mucosal lymphocytes to the microvessels of the small intestine but not the colon under uninflamed as well as inflamed conditions PMID: 14592943
    13. Neonatal CD8+ splenocytes uniformly express alpha(E) integrin and exhibit a high responsiveness to CC chemokine ligand 25. With increasing age, the frequency of CD8+ alpha(E) integrin(+) splenocytes decreases, roughly correlating with thymic involution. PMID: 15187103
    14. Epithelial cells and venular endothelium of small intestine are immunologically positive for CCL25, a chemokine which plays a direct role in intestinal homing of IgA antibody-secreting cells by mediating their extravasation into intestinal lamina propria. PMID: 15356112
    15. Chemokine CCL25 enhances CD103-mediated adhesion to E-cadherin. PMID: 15681774
    16. demonstrate a unique pattern of regulation for CCL25 and suggest a role for caudal type homeo box proteins in regulating CCL25 transcription PMID: 16517733
    17. Intracellular signaling required for Ccl25-stimulated T cell adhesion mediated by the integrin alpha4beta1. PMID: 17510295
    18. CCL25 is indispensable for CD8 T cells trafficking to the small intestine. PMID: 17548595
    19. Demonstrate development of intestinal inflammation in Tnf(DeltaARE) mice is critically dependent on beta7 integrin-mediated T-lymphocyte recruitment. the function of the CCL25/CCR9 axis appears dispensable in this model. PMID: 18439426
    20. CCL25 increases thymopoiesis after androgen withdrawal PMID: 18694999
    21. Ovarian CC chemokine thymus-expressed chemokine (TECK) appears to be a chemoattractant for CD8 alpha alpha-positive cells. PMID: 19109193

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 組織特異性：
    Specifically expressed by thymic dendritic cells. High levels in thymus and small intestine.
  • 數(shù)據(jù)庫鏈接：

    KEGG: mmu:20300

    STRING: 10090.ENSMUSP00000024004

    UniGene: Mm.7275