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  4. Mouse Calcitonin,CT ELISA Kit

Mouse Calcitonin,CT ELISA Kit

  • 中文名稱：
    小鼠降鈣素(CT)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E05133m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠降鈣素(CT)酶聯(lián)免疫試劑盒（CSB-E05133m）檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的CALCA含量。CALCA 是重要靶點(diǎn)，它編碼降鈣素基因相關(guān)肽等，在痛覺傳導(dǎo)、血管調(diào)節(jié)等生理過(guò)程起關(guān)鍵作用。研究機(jī)制上，聚焦其信號(hào)通路及受體相互作用，旨在通過(guò)藥物干預(yù)該靶點(diǎn)，為偏頭痛、心血管病等疾病治療提供新思路。試劑盒檢測(cè)范圍為12.5 pg/mL-800 pg/mL，本產(chǎn)品專為科研實(shí)驗(yàn)設(shè)計(jì)，可廣泛應(yīng)用于小鼠疾病模型構(gòu)建、細(xì)胞因子調(diào)控機(jī)制研究、藥物干預(yù)效果評(píng)估等基礎(chǔ)研究領(lǐng)域，為代謝性疾病及骨骼系統(tǒng)研究提供可靠檢測(cè)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名：
    CALCA Calcitonin
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    12.5 pg/mL-800 pg/mL
  • 靈敏度：
    3.12 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 84  
    Range % 80-88  
    1:2 Average % 95  
    Range % 91-99  
    1:4 Average % 86  
    Range % 83-89  
    1:8 Average % 97  
    Range % 92-102  
  • 回收率：
    The recovery of mouse CT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 87 82-93  
    EDTA plasma (n=4) 100 95-105  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    800 2.026 2.068 2.047 1.945  
    400 1.412 1.465 1.439 1.337  
    200 0.908 0.935 0.922 0.820  
    100 0.568 0.534 0.551 0.449  
    50 0.346 0.326 0.336 0.234  
    25 0.269 0.268 0.269 0.167  
    12.5 0.189 0.198 0.194 0.092  
    0 0.101 0.103 0.102    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Calcitonin family
  • 數(shù)據(jù)庫(kù)鏈接：