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  4. Mouse Cardiotrophin-like cytokine factor 1(CLCF1) ELISA kit

Mouse Cardiotrophin-like cytokine factor 1(CLCF1) ELISA kit

  • 中文名稱：
    小鼠心臟營(yíng)養(yǎng)素樣細(xì)胞因子1(CLCF1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL005479MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠心臟營(yíng)養(yǎng)素樣細(xì)胞因子1(CLCF1)酶聯(lián)免疫試劑盒（CSB-EL005479MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的CLCF1含量。CLCF1 即睫狀神經(jīng)營(yíng)養(yǎng)因子樣細(xì)胞因子 1，可參與多種生理病理過(guò)程。在研究機(jī)制方面，它能與細(xì)胞表面受體結(jié)合，激活下游信號(hào)通路，影響細(xì)胞生長(zhǎng)、分化、存活等。對(duì)其深入研究有望為相關(guān)疾病如神經(jīng)系統(tǒng)疾病、癌癥等治療提供新靶點(diǎn)。試劑盒檢測(cè)范圍為23.5 pg/mL-1500 pg/mL，該產(chǎn)品可廣泛應(yīng)用于基礎(chǔ)科研領(lǐng)域，如心血管疾病動(dòng)物模型中CLCF1的分泌動(dòng)態(tài)研究、體外培養(yǎng)細(xì)胞因子釋放機(jī)制分析，以及心臟組織損傷修復(fù)相關(guān)的分子機(jī)制探索。實(shí)驗(yàn)操作流程標(biāo)準(zhǔn)化，兼容常規(guī)酶標(biāo)儀檢測(cè)，為研究者提供穩(wěn)定高效的CLCF1定量分析工具，滿足神經(jīng)科學(xué)、心血管研究及免疫調(diào)節(jié)等方向的實(shí)驗(yàn)需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    Clcf1 ELISA kit; Bsf3 ELISA kit; Nnt1Cardiotrophin-like cytokine factor 1 ELISA kit; B-cell-stimulating factor 3 ELISA kit; BSF-3 ELISA kit; Novel neurotrophin-1 ELISA kit; NNT-1 ELISA kit
  • 縮寫：
    CLCF1
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    23.5 pg/mL-1500 pg/mL
  • 靈敏度：
    5.8 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CLCF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 90
    Range % 85-94
    1:2 Average % 89
    Range % 85-93
    1:4 Average % 103
    Range % 98-109
    1:8 Average % 85
    Range % 80-90
  • 回收率：
    The recovery of mouse CLCF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 96 91-100
    EDTA plasma (n=4) 91 85-97
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1500 2.401 2.293 2.347 2.204
    750 1.927 1.876 1.902 1.759
    375 1.315 1.361 1.338 1.195
    187.5 0.869 0.837 0.853 0.710
    94 0.574 0.584 0.579 0.436
    47 0.338 0.319 0.329 0.186
    23.5 0.232 0.245 0.239 0.096
    0 0.146 0.140 0.143 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    In complex with CRLF1, forms a heterodimeric neurotropic cytokine that plays a crucial role during neuronal development. Also stimulates B-cells. Binds to and activates the ILST/gp130 receptor.
  • 基因功能參考文獻(xiàn)：
    1. Cardiotrophin-like cytokine factor 1 (CLCF1) and neuropoietin (NP) signalling and their roles in development, adulthood, cancer and degenerative disorders. PMID: 26198769
    2. CLC plays a role in astrocyte differentiation via STAT3 activation within the developing brain PMID: 12090754
    3. IL-6 (gp130) family cytokine members include CLC/NNT1/BSF3 and IL-27 [review] PMID: 15253382
    4. In this study, Clc knockout mice had decreased facial motility, did not suckle, died within 24 hours, and had 32% and 29% fewer motor neurons in the facial nucleus and lumbar ventral horn, respectively. PMID: 19098279
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    IL-6 superfamily
  • 數(shù)據(jù)庫(kù)鏈接：