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  4. Mouse Carnitine O-palmitoyltransferase 1, liver isoform(CPT1A) ELISA kit

Mouse Carnitine O-palmitoyltransferase 1, liver isoform(CPT1A) ELISA kit

  • 中文名稱：
    小鼠肉毒堿-棕櫚?；D移酶1，肝臟亞型(CPT1A)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL005922MO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠肉毒堿-棕櫚?；D移酶1，肝臟亞型(CPT1A)酶聯(lián)免疫試劑盒（CSB-EL005922MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、細胞裂解物、組織勻漿樣本中的CPT1A含量。CPT1A是一種重要的靶點。它在脂肪酸代謝中扮演關鍵角色，催化長鏈脂肪酸轉運入線粒體進行β-氧化。對其研究發(fā)現(xiàn)，異常的CPT1A活性與多種疾病如代謝綜合征、癌癥等相關，探索其作用機制有望為相關疾病治療提供新方向。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，該產(chǎn)品可應用于基礎醫(yī)學研究中肝臟代謝功能評估、肥胖或糖尿病動物模型能量代謝通路分析、藥物干預對脂肪酸氧化影響的體外實驗等領域，為探索脂質代謝調控機制及相關疾病發(fā)病機理提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Cpt1a; Cpt-1; Cpt1; Carnitine O-palmitoyltransferase 1, liver isoform; CPT1-L; Carnitine O-palmitoyltransferase I, liver isoform; CPT I; CPTI-L; Carnitine palmitoyltransferase 1A
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell lysates, tissue homogenates
  • 檢測范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CPT1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %89
    Range %83-100
    1:2Average %96
    Range %87-102
    1:4Average %90
    Range %85-99
    1:8Average %93
    Range %87-99
  • 回收率：
    The recovery of mouse CPT1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9786-104
    EDTA plasma (n=4)9389-97
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.006 2.034 2.020 1.843
    2001.372 1.385 1.379 1.202
    1000.735 0.765 0.750 0.573
    500.431 0.442 0.437 0.260
    250.286 0.291 0.289 0.112
    12.50.237 0.248 0.243 0.066
    6.250.215 0.223 0.219 0.042
    00.174 0.179 0.177
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Plays an important role in hepatic triglyceride metabolism.
  • 基因功能參考文獻：
    1. Alogliptin increased hepatic mRNA expression levels associated with fatty acid oxidation. In addition, the results of the present study suggested that ALO promotes CPT1a expression via Thr172 phosphorylation of AMPKalpha. PMID: 29512720
    2. Furthermore, given the already low abundance of Cpt1b in white adipose tissue, it is unlikely that decreases in its expression can quantitatively decrease whole body energy expenditure enough to contribute to an obese phenotype. PMID: 28330968
    3. beneficial effects of muscle CPT1mt expression suggest that a direct modulation of the malonyl-CoA/CPT1 partnership in skeletal muscle could represent a potential strategy to prevent obesity-induced insulin resistance PMID: 27507552
    4. The data suggest that AMPK is not required for the regulation of the intermediate filament interaction with CPT-I during exercise. PMID: 27941154
    5. These results suggest that n-3 polyunsaturated fatty acids could improve lipid oxidation-related enzymes in liver subjected to hemorrhagic shock/resuscitation. PMID: 27110821
    6. these results demonstrated that L-carnitine ameliorated liver inflammation and serum pro-inflammatory markers in cancer cachexia through regulating CPT I-dependent PPARg signaling PMID: 26647854
    7. Data show that peroxisome-proliferator-activated receptor beta/delta (PPARbeta/delta) activation restored the lipid-induced endothelial dysfunction by up-regulation of carnitine palmitoyltransferase)-1 (CPT-1). PMID: 26253087
    8. Our results indicated that exercise-induced CPT1b expression was at least in part mediated by HDAC5/MEF2A interaction. PMID: 25213552
    9. Data indicate that muscle mutated carnitine palmitoyltransferase 1 (CPT1mt) expression enhanced mitochondrial fatty acid oxidation (mFAO) capacity. PMID: 25713059
    10. Potential epigenetic mechanisms underlying transcriptional regulation of Cpt1alpha in hepatic steatosis occur in response to binge ethanol administration. PMID: 23905631
    11. an environment-dependent structural switch underlies the regulation of carnitine palmitoyltransferase 1A PMID: 21990363
    12. miR-370 acting via miR-122 may have a causative role in the accumulation of hepatic triglycerides by modulating initially the expression of SREBP-1c, DGAT2, and Cpt1alpha. PMID: 20124555
    13. Results show that CREB and HNF4alpha bind the CPT promoter, that PGC-1 is involved in liver carnitine palmitoyltransferase I (CPT) gene activation by cylic AMP, and that PGC-1 acts to coordinate the process of metabolic adaptation in the liver. PMID: 12107181
    14. role of thyroid hormone response unit formed between the promoter and first intron in mediating liver-specific induction by thyroid hormone PMID: 12493735
    15. Malonyl-CoA/CPTI interaction is a component of a metabolic signaling network that controls insulin secretion. PMID: 15677504
    16. modulation of CPT1A expression by PI3K-dependent signaling is the major mechanism by which cells suppress beta-oxidation during anabolic growth PMID: 17030509
    17. These results suggested that astaxanthin promoted lipid metabolism rather than glucose utilization during exercise via CPT I activation, which led to improvement of endurance and efficient reduction of adipose tissue with training. PMID: 18082622

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  • 亞細胞定位：
    Mitochondrion outer membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Carnitine/choline acetyltransferase family
  • 數(shù)據(jù)庫鏈接：