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Mouse Cartilage oligomeric matrix protein(COMP) ELISA kit

  • 中文名稱:
    小鼠軟骨寡聚基質(zhì)蛋白(COMP)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-EL005778MO
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    小鼠軟骨寡聚基質(zhì)蛋白(COMP)酶聯(lián)免疫試劑盒(CSB-EL005778MO)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的COMP含量。試劑盒檢測(cè)范圍為6.25 ng/mL-400 ng/mL,適用于小鼠骨骼疾病模型(如基因敲除模型、藥物干預(yù)模型)中COMP蛋白水平的動(dòng)態(tài)監(jiān)測(cè),為軟骨代謝機(jī)制研究、骨關(guān)節(jié)損傷修復(fù)評(píng)估等科研領(lǐng)域提供可靠工具,滿足科研實(shí)驗(yàn)室對(duì)骨關(guān)節(jié)生物標(biāo)志物的高通量檢測(cè)需求本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    CompCartilage oligomeric matrix protein ELISA kit; COMP ELISA kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    6.25 ng/mL-400 ng/mL
  • 靈敏度:
    1.56 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長:
    450 nm
  • 研究領(lǐng)域:
    Cell Biology
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse COMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %91
    Range %86-95
    1:2Average %102
    Range %97-107
    1:4Average %91
    Range %85-97
    1:8Average %97
    Range %91-103
  • 回收率:
    The recovery of mouse COMP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-98
    EDTA plasma (n=4)9790-100
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    4002.512 2.600 2.556 2.370
    2001.680 1.571 1.626 1.440
    1000.955 0.962 0.959 0.773
    500.576 0.561 0.569 0.383
    250.394 0.384 0.389 0.203
    12.50.313 0.324 0.319 0.133
    6.250.259 0.264 0.262 0.076
    00.187 0.185 0.186
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7.
  • 基因功能參考文獻(xiàn):
    1. COMP could normally have a protective role against PASMC phenotype switching PMID: 28860005
    2. these findings revealed the essential role of COMP in retarding the development of vascular aging and vascular smooth muscle cell senescence. PMID: 27498005
    3. COMP deficiency drove macrophages toward the atherogenic phenotype and thereby aggravated atherosclerotic calcification. PMID: 27151399
    4. COMP forms a complex with collagens intracellularly that is a prerequisite for collagen secretion. PMID: 26746240
    5. The accumulation and thereby the functionality of thrombospondin in extracellular matrix is controlled by concentration-dependent, intermolecular "matrix trapping" mechanism. PMID: 25995382
    6. COMP deficiency shortened tail-bleeding and clotting time and accelerated ferric-chloride-induced thrombosis. COMP specifically inhibited thrombin-induced platelet aggregation, activation, and retraction and the thrombin-mediated cleavage of fibrinogen. PMID: 26045608
    7. COMP immunoreactivity was observed in about half of the investigated plaques from the ApoE null mice, mainly located along the intima-medial border. Plaques in the brachiocephalic artery from ApoE mice lacking COMP were increased in size with 54%. PMID: 25133350
    8. study will facilitate better awareness of the differential diagnoses that might be associated with the PSACH/MED spectrum and subsequent care of PSACH/MED patients PMID: 24312420
    9. The lack of arthritis, together with high levels of COMP-specific antibodies, in COMP-deficient mice indicates that susceptibility to arthritis is COMP specific and that endogenous expression of COMP in wild-type mice tolerizes B cells in vivo. PMID: 23754310
    10. results imply that COMP is not a key upstream mediator of the anabolic effects of ML on the skeleton. PMID: 23098652
    11. Lack of COMP and matrilin 3 leads to increased deposition of TIMP-3, which causes partial inactivation of matrix metalloproteinases in bone, including MMP-13. PMID: 22378539
    12. A novel form of chondrocyte stress triggered by the expression of a human-like mutation in COMP is central to the pathogenesis of pseudoachondroplasia. PMID: 22006726
    13. reducing steady state levels of COMP mRNA alleviates intracellular retention of other extracellular matrix proteins associated with the pseudoachondroplasia cellular pathology PMID: 20421976
    14. Data show that cartilage oligomeric matrix protein (COMP) promotes cell attachment via independent mechanisms involving cell surface CD47 and alphaVbeta3 integrin and that cell attachment to COMP induces formation of fascin-stabilized actin microspikes. PMID: 20033473
    15. Cartilage oligomeric matrix protein-deficient mice have normal skeletal development. PMID: 12024046
    16. Comp was expressed in tendon clone cells. PMID: 12837285
    17. Negative regulation of transcription is an important mechanism for chondrocyte-specific expression of the COMP gene PMID: 15183430
    18. ADAMTS-7 is the first metalloproteinase found to bind directly to and degrade COMP PMID: 16585064
    19. COMP appears to be required for granulin PC cell-derived growth factor-mediated chondrocyte proliferation PMID: 17307734
    20. a mutation in the C-terminal domain of COMP exerts a dominant-negative effect on both intra- and extracellular processes. PMID: 17588960
    21. contribution of COMP to the phenotype of mice deficient in both collagen IX and COMP appears minor, even though clear differences in the deposition of matrilin-3 were detected PMID: 18191556
    22. COMP-deficient mice develop an early onset collagen-induced arthritis and more severe arthritis during the chronic phase. PMID: 19014566

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  • 亞細(xì)胞定位:
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族:
    Thrombospondin family
  • 組織特異性:
    Expressed only in cartilage, including nasal, knee epiphyseal and rib tissues.
  • 數(shù)據(jù)庫鏈接: