Mouse Catalase (CAT) ELISA Kit
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中文名稱:小鼠過氧化氫酶(CAT)酶聯(lián)免疫試劑盒
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貨號:CSB-E14190m
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規(guī)格:96T/48T
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價(jià)格:¥3800/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:小鼠過氧化氫酶(CAT)酶聯(lián)免疫試劑盒(CSB-E14190m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的CAT含量。靶點(diǎn)研究是醫(yī)藥領(lǐng)域的關(guān)鍵環(huán)節(jié),通過深入了解疾病分子機(jī)制和生物學(xué)特性,尋找新的藥物靶點(diǎn),設(shè)計(jì)精確高效的藥物治療方案。研究機(jī)制包括基因組學(xué)、蛋白質(zhì)組學(xué)和生物信息學(xué),以及高通量篩選技術(shù)。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL,適用于體外培養(yǎng)細(xì)胞模型中的抗氧化能力評估、動(dòng)物實(shí)驗(yàn)中組織氧化應(yīng)激水平分析,以及疾病模型(如代謝綜合征、神經(jīng)退行性疾?。┲醒趸瘬p傷機(jī)制的探索性研究,為生命科學(xué)基礎(chǔ)研究提供可靠的檢測工具本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:Cat ELISA kit; Cas-1 ELISA kit; Cas1Catalase ELISA kit; EC 1.11.1.6 ELISA kit
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縮寫:
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, cell culture supernates, tissue homogenates
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檢測范圍:15.6 pg/mL-1000 pg/mL
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靈敏度:3.9 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Cancer
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1000 Average % 87 Range % 82-93 1:2000 Average % 93 Range % 84-99 1:4000 Average % 98 Range % 94-106 1:8000 Average % 93 Range % 86-98 -
回收率:
The recovery of mouse CAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 89-98 EDTA plasma (n=4) 92 87-96 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 1000 2.403 2.453 2.428 2.291 500 2.178 2.196 2.187 2.050 250 1.745 1.787 1.766 1.629 125 1.217 1.309 1.263 1.126 62.5 0.761 0.778 0.770 0.633 31.2 0.482 0.499 0.491 0.354 15.6 0.312 0.333 0.323 0.186 0 0.138 0.136 0.137 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Gui-zhi-fu-ling-wan alleviates bleomycin-induced pulmonary fibrosis through inhibiting epithelial-mesenchymal transition and ferroptosis ZY Chen, MM Ma, R Wang, QQ Zhang,Frontiers in Pharmacology,2025
- Integrating oxygen-boosted sonodynamic therapy and ferroptosis via engineered exosomes for effective cancer treatment M Wu, Z Zhang, D Li, X Ruan, J Yang, S Chen,Theranostics,2025
- Hesperidin Nanoformulation: A Potential Strategy for Reducing Doxorubicin-Induced Renal Damage via the Sirt-1/HIF1-α/VEGF/NF-κB Signaling Cascade FA Alherz, TA El-Masry, GA Oriquat, E Elekhnawy,Pharmaceuticals,2024
- Walnut extract protects against hepatic inflammation and toxicity induced by a high‐fat diet G Ali, A Zeb, M Usman, S Al‐Babili,Food Science & Nutrition,2024
- Effects of elafibranor on liver fibrosis and gut barrier function in a mouse model of alcohol-associated liver disease A Koizumi,World Journal of Gastroenterology,2024
- Vitamin D deficiency exacerbates alcohol-related liver injury via gut barrier disruption and hepatic overload of endotoxin A Shibamoto,The Journal of nutritional biochemistry,2023
- Melatonin protected against kidney impairment induced by 5‐fluorouracil in mice ME Elbanan,Journal of experimental zoology. Part A, Ecological and integrative physiology,2023
- Oxidative stress and mitochondrial dysfunction in brain of vinclozolin exposed animals VK Verma,Neurochemistry international,2024
- Interaction between dual specificity phosphatase-1 and cullin-1 attenuates alcohol-related liver disease by restoring p62-mediated mitophagy R Li,International journal of biological sciences,2023
- Development of skin health test efficacy modules in vivo YH Lin,International Journal of Scientific Research Updates,2022
- Pyrvinium pamoate ameliorates cyclosporin A-induced hepatotoxicity via the modulation of Wnt/β-catenin signaling and upregulation of PPAR- SA Faheem,International Immunopharmacology,2022
- Combatting Nitrosative Stress and Inflammation with Novel Substituted Triazinoindole Inhibitors of Aldose Reductase in PC12 Cells Exposed to 6-Hydroxydopamine Plus High Glucose Z Elmazoglu,Neurotoxicity Research,2020
- Nrf2-ARE signals mediated the anti-oxidative action of electro-acupuncture in an MPTP mouse model of Parkinson´s disease Lv E. et al,Free Radic Res.,2015
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells.
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基因功能參考文獻(xiàn):
- It has been shown that genetic ablation of Nrf2 abolishes an adaptive muscle NQO1 activity and catalase induction. PMID: 29402993
- Catalase overexpression modulates metabolic parameters in a new 'stress-less' leptin-deficient mouse model. PMID: 28645653
- homocysteine reacts with native catalase and/or redox-active transition metal ions to generate thiyl radicals that mediate compound II formation, a temporarily inactive state of the enzyme. PMID: 27848965
- Catalase ameliorates diabetes-induced cardiomyopathy through reduced RelA-mediated transcription of BECN1. PMID: 28643395
- Mitochondrial catalase induces neoplastic cell transformation through nucleolin-dependent Cox-2 mRNA stabilization. PMID: 29097213
- Moderate MnSOD and/or catalase overexpression in desmin-null hearts leads to a marked decrease in intracellular reactive oxygen species, ameliorates mitochondrial and other ultrastructural defects, minimizes myocardial degeneration and leads to a significant improvement of cardiac function. PMID: 28629836
- Mitochondrial catalase overexpressed transgenic mice are protected against lung fibrosis in part via preventing alveolar epithelial cell mitochondrial DNA damage. PMID: 27840320
- Mice devoid of catalase develop an obese, pre-diabetic phenotype and provide compelling evidence for catalase (or its products) being integral in metabolic regulation. PMID: 27939935
- Data indicate that alpha-syntrophin plays an important role in the regulation of oxidative stress from endogenously generated reactive oxygen species (ROS) during myoblast differentiation by modulating the protein stability of catalase. PMID: 28485891
- Overexpression of catalase reduced the concentrations of microsomal benzo(a)pyrene phenols and diols/diones by ~45 and 95%, respectively. PMID: 27607467
- Results indicate that mitochondrial catalase likely functions to preclude the formation of high levels of H2O2 without perturbing redox-dependent signaling. PMID: 27614223
- Age strongly interacts with catalase targeted to the mitochondria, consistent with antagonistic pleiotropy in the reverse of the typical direction. PMID: 27061426
- data suggest that catalase is required not only to scavenge reactive oxygen species, but also to protect DNA from oxidative damage during meiotic maturation in mouse oocytes. PMID: 27160095
- These results indicate that CAT expression is significantly decreased in the hippocampi of aged animals and decreased CAT expression may be closely associated with aging. PMID: 27221506
- catalase protects mouse hearts against diabetic cardiomyopathy, partially by suppressing NF-kappaB-dependent inflammatory responses and associated protein nitration. PMID: 26456065
- Hypoxia-generated red blood cells have low catalase and are preferentially destroyed. PMID: 26017143
- The study shows how maturation of active catalase can be influenced by nitric oxide, S-nitrosylated GAPDH, and thioredoxin-1, and how maturation may become compromised in inflammatory conditions such as asthma. PMID: 25659933
- Data suggest that embryonic catalase is a determinant of risk for EtOH embryopathies. PMID: 26074427
- exposure to either 14- or 28-day chronic stress resulted in a depressive-like syndrome, behavioural invigoration and aggression, and decreased activity of two major brain peroxidation enzymes, superoxide dismutase and catalase. PMID: 24786329
- The antioxidant catalase counteracts against high fat diet-induced cardiac geometric and functional anomalies possibly via an IKKbeta-AMPK-dependent restoration of myocardial autophagy. PMID: 24993069
- The SirT1 regulates the expression of several antioxidant genes in bovine aortic endothelial cells, including Mn superoxide dismutase, catalase, peroxiredoxins 3 and 5, thioredoxin 2, thioredoxin reductase 2, and uncoupling protein 2. PMID: 23461683
- The levels of protein carbonyls, reactive oxygen species, and catalase in the cerebral hemispheres of young and aged mice, were evaluated. PMID: 24338129
- The expression levels of catalase and SOD2 are decreased in astrocytes when SIRT1 is inhibited. PMID: 24565075
- Data indicate that transgenic mice over-expressing human SOD1 or catalase were protected from loss of plasma membrane integrity (LPMI) at early but not late periods of reperfusion. PMID: 23911407
- Data indicate no significant difference in CAT and CuZnSOD gene expression, but enzymatic activity of CuZnSOD and MnSOD significantly increased after sleep deprivation. PMID: 23360182
- Up-regulation of catalase caused by high-fat diet is designed to protect heart mitochondria from oxidative damage while not perturbing H2O2-mediated signaling. PMID: 23204527
- Antioxidant enzyme, catalase, reverses DNA damage response and cellular transdifferentiation in aortic valve sclerosis. PMID: 23241403
- Myocardial catalase expression protects against endotoxemic myocardial dysfunction in mice. PMID: 22902401
- catalase overexpression in renal proximal tubular cells ameliorated maternal diabetes-induced perinatal programming, mediated in part by triggering the Nrf2-HO-1 defense system. PMID: 22733796
- The increased expression of antioxidant catalase, specifically in hippocampal neurons, will provide protection from age-related cognitive decline. PMID: 21942371
- The findings indicated that lower skin catalase activity in male mice may at least in part contribute to increased ultraviolet light B-induced generation of Gr-1+CD11b+ myeloid cells. PMID: 22030957
- endogenous catalase plays an important role in protecting the kidney from diabetic stress through maintaining peroxisomal and mitochondrial fitness. PMID: 22315314
- physiological venous catalase activity prevents exercise-induced up-regulation of endothelial nitric oxide synthase and extracellular superoxide dismutase PMID: 21129156
- Data suggest that embryonic catalase plays an important protective role, and both physiological and phenytoin-enhanced oxidative stress can be embryopathic. PMID: 21478259
- Chestnut inner shell extract treatment with ethanol decreased CYP2E1 expression and increased activities of catalase and superoxide dismutase. PMID: 21457746
- Targeting catalase within mitochondria of tumor cells and tumor stromal cells suppresses ROS-driven tumor progression and metastasis. PMID: 21605372
- The data indicate catalase levels is decreased in stem cell-like characteristics. PMID: 21347610
- catalase may rescue paraquat-induced myocardial geometric and functional alteration possibly by alleviating JNK-mediated endoplasmic reticulum stress PMID: 20937379
- There were no acute functional improvements with catalase overexpression before myocardial infarction, but sustained H(2)O(2), influenced by catalase expression, levels may be critical in directing remodeling and cardiac function at later time points. PMID: 20971939
- The effect of increased mtDNA mutations on age-dependent cardiac structural changes and functional disturbance using Polgm/m mice and the contribution of mtreactive oxygen species in these mice by crossing Catalase to Polgm/m mice, was expored. PMID: 20456298
- overexpression of catalase decreases oxidant injury in the proximal tubular cells reverses abnormalities due to enhanced generation of reactive oxygen species in the proximal tubule cells PMID: 20237455
- peroxiredoxin-2, glutathione peroxidase-1, and catalase have roles in erythrocyte oxidative metabolism PMID: 19969073
- we have demonstrated that forced catalase expression in the mitochondria enhances exercise performance. Our result provides a framework for further elucidating the underlying mechanism PMID: 19690612
- A single base pair difference in the Cas1 open reading frame between the C57BL/6J and DBA/2J strains results in Ala-117 in the D2 strain and threonine at this position in the B6 strain. PMID: 11821648
- Overexpression of catalase diminishes the pressor response to NE and Ang II by reducing H(2)O(2) production in the arterial wall. PMID: 12517675
- The regulation of this gene's expression in mouse muscle cells is dependent on the CCAAT-binding factor NF-Y. PMID: 12659863
- findings indicate that, in addition to stimulating catalase activity, c-Abl and Arg promote catalase degradation in the oxidative stress response PMID: 12950161
- overexpression of catalase delays or prevents alterations in phospholipid metabolism during glucocorticoid-induced apoptosis PMID: 14572898
- effect of IGFBP-2 overexpression on the activity of catalas in two malignant cell lines PMID: 14710363
- the role of catalase in antioxidant defense is dependent on the type of tissue and the model of oxidant-mediated tissue injury PMID: 15178682
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亞細(xì)胞定位:Peroxisome.
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蛋白家族:Catalase family
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