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  4. Mouse Chitinase-3-like protein 1(CHI3L1) ELISA kit

Mouse Chitinase-3-like protein 1(CHI3L1) ELISA kit

  • 中文名稱：
    小鼠幾丁質(zhì)酶3樣蛋白1(CHI3L1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL005346MO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠幾丁質(zhì)酶3樣蛋白1(CHI3L1)酶聯(lián)免疫試劑盒（CSB-EL005346MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的CHI3L1含量。CHI3L1是一個重要的靶點。它在生理和病理過程中發(fā)揮作用，與炎癥、纖維化等密切相關。研究機制方面，主要聚焦于其在細胞信號傳導、免疫調(diào)節(jié)等方面的功能，探索其在疾病發(fā)生發(fā)展中的作用及潛在的治療干預方式。試劑盒檢測范圍為0.47 ng/mL-30 ng/mL，本試劑盒適用于科研場景中探索CHI3L1在疾病模型中的表達動態(tài)，例如評估炎癥性疾病或纖維化動物模型中該蛋白的分泌水平變化，亦可應用于體外細胞實驗中對藥物干預或基因調(diào)控后CHI3L1釋放量的定量分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Chi3l1 ELISA Kit; Brp39 ELISA Kit; Chil1 ELISA Kit; Chitinase-3-like protein 1 ELISA Kit; BRP39 protein ELISA Kit; Breast regression protein 39 ELISA Kit; Cartilage glycoprotein 39 ELISA Kit; CGP-39 ELISA Kit; GP-39 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    0.47 ng/mL-30 ng/mL
  • 靈敏度：
    0.11 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CHI3L1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 84
    Range % 80-88
    1:2 Average % 91
    Range % 86-95
    1:4 Average % 103
    Range % 99-107
    1:8 Average % 94
    Range % 85-98
  • 回收率：
    The recovery of mouse CHI3L1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 89 85-93
    EDTA plasma (n=4) 95 90-100
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    30 2.601 2.476 2.539 2.319
    15 1.933 1.819 1.876 1.656
    7.5 1.140 1.204 1.172 0.952
    3.75 0.692 0.682 0.687 0.467
    1.88 0.457 0.441 0.449 0.229
    0.94 0.349 0.358 0.354 0.134
    0.47 0.284 0.297 0.291 0.071
    0 0.224 0.215 0.220 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.
  • 基因功能參考文獻：
    1. Chi3l1 is a regulator of Th1 and CTL which could be a therapeutic target to enhance anti-tumor immunity. PMID: 29403003
    2. Nrf2/Keap1 system regulates VSMC apoptosis during neointimal formation, thereby inhibiting neointimal hyperplasia after a vascular injury. PMID: 27198666
    3. Knockdown of Chi3l1 in the arterial endothelium suppressed the development of atherosclerosis in amyloid precursor protein transgenic mice. PMID: 29344304
    4. CHI3L1 may be a previously unrecognized biomarker for diagnosing Gaucher Diseases (GD) and for evaluating the therapeutic effects of new GD drug. PMID: 29396296
    5. CHI3L1 deficiency in donor splenocytes dramatically increased the severity of aGVHD through enhancing Tfh cell differentiation PMID: 28656528
    6. miR-24 serves as a molecular regulator in S. aureus-induced macrophage polarization through targeting of CHI3L1 and regulation of the MAPK pathway PMID: 28303416
    7. The mouse model was used to validate the function of CHI3L1 in cancer metastasis in vivo PMID: 28143526
    8. CHI3L1-mediated cell proliferation/survival involves partial downregulation of the pro-apoptotic S100A9 protein that is highly expressed during the acute phase of colitis, by binding to the S100A9 receptor, RAGE (Receptor for Advanced Glycation End products). PMID: 26431492
    9. IL-18 is a potent stimulator of Chi3l1 and that Chi3l1 is an important mediator of IL-18-induced inflammatory, fibrotic, alveolar remodeling, and cytotoxic responses. PMID: 25955511
    10. CHI3L1 is induced during fungal infection, and it regulated antifungal innate immune responses in the cornea. PMID: 26238714
    11. Astrocytic expression of chitinase 3-like protein 1 limits the extent of both astrocytic and microglial/macrophage facets of neuroinflammation. PMID: 25377763
    12. Results do not support a role of BRP-39 in the pathogenesis of experimental autoimmune encephalitis. PMID: 26079949
    13. CHI3L1 was increased in plasma and brain tissue in experimental cerebral malaria, but targeted Chi3l1 deletion did not alter cytokine production or survival in this model. PMID: 25047113
    14. mechanism of BRP-39 in bleomycin-induced pulmonary fibrosis PMID: 24659093
    15. Sem7a and its receptors regulate Chi3l1 in pulmonary melanoma metastasis PMID: 25511377
    16. Data show that IL-1-induced transcriptin factor RelB/NF-kappa B p50 Subunit complex formation was further promoted by oncostatin M and that these complexes directly bound to the secreted protein cartilage glycoprotein 39 (YKL-40) promoter. PMID: 25681350
    17. These results indicate that catalytically inactive BRP-39 and Ym1 are constitutive genes in normal mouse lung. PMID: 25294623
    18. BRP-39 is involved in repressing the M1 proinflammatory phenotype in hyperoxia, thereby deactivating inflammatory responses in macrophages and preventing neonatal lung injury. PMID: 24347826
    19. Chi3l1 binds to IL-13Ralpha2, stimulates MAPK, Akt/PKB, Wnt signaling and regulates melanoma metastasis. PMID: 23972995
    20. Stat3 downstream gene product chitinase 3-like 1 is elevated in lung cancer PMID: 23613996
    21. We identified the macrophage-secreted chitinase-like protein Brp-39 as a critical component of this reparative response that serves to limit tubular cell apoptotic death via activation of Akt, improving animal survival after kidney ischemia/reperfusion. PMID: 23291472
    22. BRP-39-deficient mice demonstrate the importance of BRP-39 in modulating the severity of clinical experimental autoimmune encephalomyelitis and central nervous system neuroinflammation. PMID: 23041842
    23. Chitinase 3-like-1 is induced by Streptococcus pneumoniae and plays central roles in promoting bacterial clearance and mediating host tolerance. PMID: 22817986
    24. CHI3L1 up-regulates pro-inflammatory mediators, C-chemokine ligand 2, chemokine CX motif ligand 2 and matrix metalloproteinase-9 all of which contribute to tumor growth and metastasis; study shows that CHI3L1 plays a role in tumor progression PMID: 21866546
    25. YKL-40-mediated inhibition of cell differentiation and polarization in the presence of lactogenic hormones may represent its important function during mammary tissue involution. PMID: 21991364
    26. YKL-40/BRP-39 levels are increased in human and murine CF airway fluids PMID: 21949714
    27. The carbohydrate-binding region of CHI3L1 is critical in activating Akt signaling in colon epithelial cells, and the activation may be associated with the development of chronic colitis. PMID: 21546314
    28. breast regression protein-39 has a role in the pathogenesis of cigarette smoke-induced inflammation and emphysema PMID: 20656949
    29. BRP-39 is functionally important in house dust mite-induced inflammation. BRP-39 is a biomarker of cigarette smoke induced inflammation which is the byproduct of an IL-1 inflammatory pathway. PMID: 21473774
    30. pathogenic role in colitis, presumably by enhancing the adhesion and invasion of bacteria on/into colonic epithelial cells. PMID: 16472595
    31. Cloning, expression, characterization and crystallization of BRP39, a signalling glycoprotein expressed during mammary gland apoptosis PMID: 19041398
    32. BRP-39 deficient mice show diminished antigen-induced Th2 cell responses, rescued by YKL-40 administration. PMID: 19414556

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  • 亞細胞定位：
    Secreted, extracellular space. Cytoplasm. Endoplasmic reticulum. Note=Detected in bronchoalveolar lavage (BAL) fluids. Localizes mainly to endoplasmic reticulum when overexpressed in cells, with some protein also detected throughout the cytoplasm.
  • 蛋白家族：
    Glycosyl hydrolase 18 family
  • 組織特異性：
    Detected in lung in pulmonary macrophages and alveolar type 2 cells and in bronchoalveolar lavage (BAL) fluids. Expressed in mammary tumor cells (at protein level). Expressed in lung. Not detected in non-inflammatory colon.
  • 數(shù)據(jù)庫鏈接：