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  4. Mouse Claudin-5(CLDN5) ELISA kit

Mouse Claudin-5(CLDN5) ELISA kit

  • 中文名稱：
    小鼠封閉蛋白5(CLDN5)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL005507MO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠封閉蛋白5(CLDN5)酶聯(lián)免疫試劑盒（CSB-EL005507MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的CLDN5含量。CLDN5即閉合蛋白5，是緊密連接蛋白家族成員，在血管內(nèi)皮細胞廣泛表達，對維持血腦屏障完整性至關(guān)重要。研究發(fā)現(xiàn)其可作為腫瘤治療靶點，通過調(diào)節(jié)其表達或功能，影響腫瘤血管生成和轉(zhuǎn)移，為腫瘤診斷與治療提供新思路。試劑盒檢測范圍為18.75 pg/mL-1200 pg/mL，本試劑盒通過特異性捕獲抗體與檢測抗體的協(xié)同作用，可實現(xiàn)目標蛋白的高效識別與定量分析，適用于生命科學(xué)研究中探索CLDN5在組織屏障功能調(diào)控、疾病模型建立、藥物干預(yù)效果評估等領(lǐng)域的表達變化規(guī)律。為研究者提供了一種穩(wěn)定、可靠的檢測工具，適用于體外實驗?zāi)Ｐ椭蠧LDN5蛋白水平的動態(tài)監(jiān)測及相關(guān)分子機制研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Cldn5; Bec1; Claudin-5; Brain endothelial cell clone 1 protein; Lung-specific membrane protein
  • 縮寫：
    CLDN5
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    18.75 pg/mL-1200 pg/mL
  • 靈敏度：
    4.68 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CLDN5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 101
    Range % 95-104
    1:2 Average % 95
    Range % 90-99
    1:4 Average % 92
    Range % 87-98
    1:8 Average % 92
    Range % 86-97
  • 回收率：
    The recovery of mouse CLDN5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 88 83-92
    EDTA plasma (n=4) 93 87-98
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1200 2.312 2.422 2.367 2.240
    600 1.846 1.966 1.906 1.779
    300 1.261 1.229 1.245 1.118
    150 0.754 0.769 0.762 0.635
    75 0.443 0.456 0.450 0.323
    37.5 0.271 0.281 0.276 0.149
    18.75 0.208 0.202 0.205 0.078
    0 0.126 0.128 0.127 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
  • 基因功能參考文獻：
    1. This study demonstrated that the claudin-5(+) leukocytes in the central nervous system during neuroinflammation: a novel role for endothelial-derived extracellular vesicles. PMID: 27852330
    2. Claudin 5 in a murine model of allergic asthma: Its implication and response to steroid treatment PMID: 26409663
    3. while MMP-9 is not essential for hypoxic-induced cerebral angiogenesis, it plays an important role in post-hypoxic vascular pruning by degrading laminin and claudin-5 PMID: 25812799
    4. Studied if circulating TGFbeta1 drives changes in tight junction protein expression and MMP9 activity following acute liver failure; Mouse brain endothelial cells were treated with recombinant rTGFbeta1 and MMP9 and claudin-5 expression was assessed. PMID: 26006017
    5. In transgenic mice carrying mutated SOD1 genes, a disrupted blood-spinal cord barrier as well as decreased levels of tight junction proteins ZO-1, occludin, and claudin-5 were detected. PMID: 25853911
    6. The aim of the present study was to further characterize the estrogen-responsive elements of claudin-5 promoter. PMID: 24846172
    7. Two independent pathways triggered by PI3K mediate early and late loss of paracellular claudin-5 expression. PMID: 25281324
    8. Comparative modelling of intramolecular interfaces in the transmembrane region of claudins led to a complete Cldn5 model. PMID: 25174580
    9. silencing of claudin-5 did significantly attenuate simvastatin-mediated endothelial cell barrier protection in response to thrombin PMID: 24028293
    10. Claudin 5 showed identical mRNA expression levels in the brains of adult and aged mice. PMID: 22120415
    11. TNFalpha acts through NFkappaB signaling and requires a conserved promoter region for the down-regulation of Cldn5 expression. PMID: 22138107
    12. In female rats, increasing age and 17beta-estradiol treatment alters the expression of ERalpha and distinct blood-brain barrier tight-junction protein isoforms (occludin and claudin-5) without altering functional paracellular permeability. PMID: 21192956
    13. These findings suggest that claudin-5 is a main claudin expressed in podocytes and that the formation of tight junctions in the nephrosis may be due to local recruitment of claudin-5 rather than due to total upregulation of the claudin protein levels. PMID: 21271259
    14. This study sought to determine the cell signaling mechanism controlling endothelial CLDN5 expression during acute lung injury. PMID: 20525806
    15. envision that substances interfering with Cldn5-mediated paracellular barrier tightening could assist an improved drug delivery into the brain because Cldn5 is essential for blood-brain barrier integrity. PMID: 20080584
    16. Describe claudin-5 as a novel estrogen target in vascular endothelium and show in vivo (brain endothelium) and in vitro (brain and heart endothelium) effects of estrogen on claudin-5 levels. PMID: 19910637
    17. tight junctions in dermal vascular endothelial cells are composed of claudin-5. PMID: 12823443
    18. claudin-5 is specifically altered in utrophin/dystrophin-deficient (double knockout, dko hearts, suggesting that alterations of the lateral membranes of cardiomyocytes PMID: 15698839
    19. Measured changes in claudin5 after cardiac arrest in mouse lung in relation to warm ischemia time and lung injury. PMID: 18281348
    20. Amyotrophic lateral sclerosis-linked superoxide dismutase 1 (SOD1) mutants with different biochemical characteristics disrupted the blood-spinal cord barrier in mice by reducing the levels of the tight junction protein claudin-5. PMID: 18344992
    21. Hydrodynamic tail vein injection of Cldn5 siRNA attenuates Cldn5 expression. PMID: 18509865
    22. Tissue-specific regulation of the claudin-5 gene via glucocorticoids and a high vulnerability of claudin-5 to TNF alpha. PMID: 18996436
    23. the pro-inflammatory chemokine CCL2 mediates brain endothelial barrier disruption during CNS inflammation PMID: 19423710
    24. CLDN5 is expressed in the seminiferous epithelium, appears to be regulated by multiple influences, and contributes to blood-testis barrier function. PMID: 19571261
    25. Disruptions of occludin and claudin-5 in brain endothelial cells in vitro and in brains of mice with acute liver failure. PMID: 19821483

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  • 亞細胞定位：
    Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Claudin family
  • 組織特異性：
    Widely expressed with highest levels in the lung.
  • 數(shù)據(jù)庫鏈接：