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  4. Mouse Coagulation Factor XIII A1 Polypeptide(F13A1)ELISA kit

Mouse Coagulation Factor XIII A1 Polypeptide(F13A1)ELISA kit

  • 中文名稱：
    小鼠凝血因子XIII A1酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13519m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠凝血因子XIII A1酶聯(lián)免疫試劑盒（CSB-E13519m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的F13A1含量。F13A1是一個(gè)重要靶點(diǎn)。它參與凝血過程，F(xiàn)13A1編碼的凝血因子XIII A亞基在穩(wěn)定纖維蛋白凝塊中起關(guān)鍵作用?？茖W(xué)家圍繞其功能和作用機(jī)制開展研究，探索通過調(diào)節(jié)它來干預(yù)凝血異常相關(guān)疾病的新策略。試劑盒檢測(cè)范圍為1.56 ng/mL-100 ng/mL，適用于科研領(lǐng)域中對(duì)凝血機(jī)制的基礎(chǔ)研究、藥物開發(fā)中凝血調(diào)節(jié)劑的體外活性評(píng)估，或心血管疾病模型動(dòng)物中凝血相關(guān)生物標(biāo)志物的動(dòng)態(tài)分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    F13a1 ELISA kit; F13aCoagulation factor XIII A chain ELISA kit; Coagulation factor XIIIa ELISA kit; EC 2.3.2.13 ELISA kit; Protein-glutamine gamma-glutamyltransferase A chain ELISA kit; Transglutaminase A chain ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    1.56 ng/mL-100 ng/mL
  • 靈敏度：
    0.39 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Blood Coagulation
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse F13A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %87
    Range %82-93
    1:200Average %95
    Range %90-99
    1:400Average %93
    Range %87-98
    1:800Average %102
    Range %96-108
  • 回收率：
    The recovery of mouse F13A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9691-102
    EDTA plasma (n=4)9893-105
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    1002.684 2.657 2.671 2.487
    502.398 2.317 2.358 2.174
    251.865 1.898 1.882 1.698
    12.51.247 1.214 1.231 1.047
    6.250.673 0.699 0.686 0.502
    3.120.488 0.498 0.493 0.309
    1.560.357 0.386 0.372 0.188
    00.183 0.184 0.184 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
  • 基因功能參考文獻(xiàn)：
    1. Factor XIII-A and TG2 regulate resorption, adipogenesis and plasma fibronectin homeostasis in bone and bone marrow. PMID: 28387755
    2. Suggest that resident macrophages maintain plasma FXIII-A and exclude the platelet lineage as a major contributor. PMID: 28596376
    3. These findings provide insight into assembly of the fibrinogen/FXIII-A2B2 complex in both physiologic and therapeutic situations. PMID: 27561317
    4. Mast cells have the ability to produce and degrade FXIIIA depending on their chymase expression profile: mast cells expressing chymase degrade FXIIIA, whereas mast cells that do not express chymase mainly produce FXIIIA PMID: 27302551
    5. A lack of factor XIII leads to prolonged clotting times, increased signs of inflammation, and elevated bacterial dissemination in streptococcal skin diseases. PMID: 27338836
    6. FXIII is a significant determinant of mucosal healing and clinical outcome following inflammatory colitis induced mucosal injury PMID: 26098308
    7. These findings expose a newly recognized, essential role for fibrin crosslinking during whole blood clot formation and consolidation and establish FXIIIa activity as a key determinant of thrombus composition and size. PMID: 26324704
    8. related to islet dysfunction during the development of type 2 diabetes PMID: 26018251
    9. 5-HT inhibits Factor XIII-A-mediated plasma fibronectin matrix assembly and crosslinking in osteoblast cultures via direct competition with transamidation PMID: 25460579
    10. High Transglutaminase factor XIII promotes arthritis through mechanisms linked to inflammation and bone erosion. PMID: 25336631
    11. Fibrinogen from mice harboring the Fibgamma390-396A mutation exhibited reduced binding to FXIII, and plasma from these mice exhibited delayed FXIII activation and fibrin crosslinking, indicating these residues mediate FXIII binding and activation. PMID: 24983320
    12. FXIII-A serves as a preadipocyte-bound proliferation/differentiation switch that mediates effects of hepatocyte-produced circulating pFN. PMID: 24934257
    13. Physiological significance of mouse TG2 and Factor XIII through identification of substrate proteins in MC3T3-E1 cells. PMID: 22045386
    14. Studies indicate that in FXIII-A-knockout mice, tail-tip bleeding times are doubled and clot stability, assessed by thromboelastography, was impaired compared with wild-type mice. PMID: 23075332
    15. FXIIIA mRNA was regulated by the MAP kinase pathway. PMID: 21959563
    16. Data indicate that FXIIIa-targeted probe signals showed good overlap with immune-fluorescent fibrin staining images. PMID: 22272319
    17. factor XIIIA transglutaminase activity regulates osteoblast matrix secretion and deposition by affecting microtubule dynamics PMID: 21283799
    18. Inhibition of TG2 and FXIIIA during early development of lesions reduced the macrophage content in the media of atherosclerotic vessels, while not affecting lesion size or geometric remodeling PMID: 20810110
    19. Knockout mouse studies suggest that plasma FXIII-A does not derive primarily (if at all) from circulating platelets and indeed may not derive from the megakaryocyte lineage. PMID: 20086247
    20. All FXIII2/2 and FXIII2/+ mice died within 5 days after MI from left ventricular (LV) rupture and had locally reduced levels of FXIII. PMID: 17183677
    21. Factor XIII A subunit deficieny is associated with impaired clot retraction PMID: 19996413
    22. impaired clot stabilization in FXIII-A mutant mice PMID: 12529747
    23. Maternal FXIII plays a critical role in uterine hemostasis and maintenance of the placenta during gestation. PMID: 12933578
    24. platelet FXIII and calpain have roles in regulating integrin alpha(IIb)beta3 adhesive function PMID: 15131115
    25. FXIII has an essential role in wound repair and remodeling PMID: 16113836
    26. analysis of the expression, localization and activity of TG2 and FXIIIA in mineralizing tissues [review] PMID: 16368540
    27. Results demonstrate that MC3T3-E1/C14 osteoblasts express two transglutaminase isoforms--TG2 and FXIIIA. PMID: 16469487
    28. the entire FXIIIA gene is expressed, thus indicating that the 37-kDa FXIIIA is not a splice variant but a product of posttranslational proteolytic processing. PMID: 17341477
    29. Carditis and fibrosis in FXIII-deficient mice might be caused by a faulty or delayed reparative process that was initiated by abnormal haemorrhagic events within heart tissue. PMID: 18278192
    30. FXIIIa supports the early survival of micrometastases by a mechanism linked to NK cell function PMID: 18315549

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  • 亞細(xì)胞定位：
    Cytoplasm. Secreted.
  • 蛋白家族：
    Transglutaminase superfamily, Transglutaminase family
  • 數(shù)據(jù)庫鏈接：