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  4. Mouse Cold-inducible RNA-binding protein(CIRBP) ELISA kit

Mouse Cold-inducible RNA-binding protein(CIRBP) ELISA kit

  • 中文名稱：
    小鼠冷誘導(dǎo)RNA結(jié)合蛋白(CIRBP)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL005440MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠冷誘導(dǎo)RNA結(jié)合蛋白(CIRBP)酶聯(lián)免疫試劑盒（CSB-EL005440MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的CIRBP含量。CIRBP（冷誘導(dǎo)RNA結(jié)合蛋白）是一種進(jìn)化上保守的RNA結(jié)合蛋白，包含RNA識(shí)別結(jié)構(gòu)域（RRM）和RGG結(jié)構(gòu)域。它在多種應(yīng)激條件下表達(dá)變化，調(diào)控轉(zhuǎn)錄、轉(zhuǎn)錄后和翻譯過程。CIRBP在細(xì)胞應(yīng)激反應(yīng)、基因毒性應(yīng)激、炎癥、神經(jīng)可塑性、干細(xì)胞特性和癌癥發(fā)生發(fā)展中發(fā)揮重要作用。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，本試劑盒適用于體外檢測(cè)小鼠模型中CIRBP的動(dòng)態(tài)表達(dá)變化，可廣泛應(yīng)用于低溫應(yīng)激模型構(gòu)建、代謝性疾病機(jī)制研究、炎癥相關(guān)信號(hào)通路探索等科研領(lǐng)域，為研究CIRBP在生理調(diào)節(jié)或病理狀態(tài)下的功能提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Cirbp ELISA kit; CirpCold-inducible RNA-binding protein ELISA kit; A18 hnRNP ELISA kit; Glycine-rich RNA-binding protein CIRP ELISA kit
  • 縮寫：
    CIRBP
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CIRBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 89
    Range % 85-94
    1:2 Average % 102
    Range % 96-107
    1:4 Average % 92
    Range % 87-98
    1:8 Average % 94
    Range % 89-98
  • 回收率：
    The recovery of mouse CIRBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 98 93-102
    EDTA plasma (n=4) 103 92-107
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 2.490 2.567 2.529 2.397
    500 2.021 2.123 2.072 1.940
    250 1.415 1.341 1.378 1.246
    125 0.791 0.782 0.787 0.655
    62.5 0.469 0.473 0.471 0.339
    31.25 0.289 0.271 0.280 0.148
    15.6 0.210 0.214 0.212 0.080
    0 0.131 0.132 0.132 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Promotes assembly of stress granules (SGs), when overexpressed. Seems to play an essential role in cold-induced suppression of cell proliferation. Acts as a translational repressor. Acts as a translational activator. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN.
  • 基因功能參考文獻(xiàn)：
    1. CIRP induces lung endoplasmic reticulum stress and downstream responses to cause sepsis-associated acute lung injury. PMID: 28128330
    2. Study demonstrates CIRP-induced endothelial cell (EC)pyroptosis in the lungs of C57BL/6 mice for the first time. CIRP stimulates the assembly and activation of Nlrp3 inflammasome in EC accompanied with caspase-1 activation, IL-1beta release and induction of proinflammatory cell death pyroptosis. PMID: 27217302
    3. tissue damage induced NADPH oxidase activation and increased the release of reactive oxygen species via cold-inducible RNA-binding protein (CIRP)-TLR4-MyD88 signaling. PMID: 28492546
    4. temperature-dependent accumulation of Cirbp mRNA is controlled primarily by the regulation of splicing efficiency, defined as the fraction of Cirbp pre-mRNA processed into mature mRNA PMID: 27633015
    5. this paper shows that cold air stimulation induced MUC5AC expression in wild-type mice but not in CIRP-/-) mice. PMID: 27423012
    6. state. These data collectively suggest that a deficiency in CIRP accelerates the wound healing process PMID: 26743936
    7. These results can provide new insights into the molecular mechanisms of Cirp function. PMID: 25597958
    8. Findings suggest that CIRP could exert protective effects against oxidative stress, and that it might be a novel neuroprotective agent PMID: 25498861
    9. targeting CIRP offers potential therapeutic implications in the treatment of hepatic I/R injury. PMID: 25186836
    10. Cirp appears to play a critical carcinogenic function and its expression might be a useful biomarker for hepatocellular carcinomas risk prediction. PMID: 25611373
    11. Cirp promotes the development of intestinal inflammation and colorectal tumors through regulating apoptosis and production of TNFalpha and IL23 in inflammatory cells. PMID: 25187386
    12. alcohol exposure activates microglia to produce and secrete CIRP PMID: 24223948
    13. Extracellular CIRP is a detrimental factor in stimulating inflammation to cause neuronal damage in cerebral ischemia PMID: 24613680
    14. Down-regulated CIRP is involved in testicular injury after testicular torsion/detorsion. PMID: 24094970
    15. Depletion of Cirbp is found to increase the susceptibility of cells to the TNF-mediated inhibition of high amplitude expression of clock genes and modulates the TNF-induced cytokine response. PMID: 24337574
    16. In animal models of hemorrhage and sepsis, CIRP is upregulated in the heart and liver and released into the circulation. In macrophages under hypoxic stress, CIRP translocates from the nucleus to the cytosol and is released. PMID: 24097189
    17. Moderate hypothermia resulted in significant up-regulation of both RBM3 and CIRP mRNA in murine organotypic hippocampal slice cultures PMID: 23415676
    18. we discovered that downregulation of CIRP resulted in increased germ cell apoptosis, possibly via the activation of the p44/p42, p38 and SAPK/JNK MAPK pathways PMID: 23001445
    19. mRNAs binding with CIRP in testis were mostly associated with translation regulator activity, antioxidant activity, envelope and reproduction, including important mRNAs related to male infertility. PMID: 22819822
    20. CIRP confers robustness to circadian oscillators through regulation of CLOCK expression. PMID: 22923437
    21. Cirp functions to fine-tune the proliferation of undifferentiated spermatogonia by interacting with Dyrk1b. PMID: 22711815
    22. These data suggest that mild hypothermia protects cells from TNF-alpha-induced apoptosis, at least partly, via induction of Cirp, and that Cirp protects cells by activating the ERK pathway. PMID: 16569452
    23. These findings further reveal the diversity of mechanisms by which CIRP is regulated by environmental stresses and provide new insights into CIRP cytoplasmic function. PMID: 17967451
    24. CIRP enhanced extracellular signal-regulated kinase 1 and 2 (ERK1/2) phosphorylation, and treatment with an MEK inhibitor decreased the proliferation caused by CIRP. PMID: 19158277
    25. Overexpression of CIRP may reduce testicular damage induced by cryptorchidism by down-regulating the levels of p53 and Fas. PMID: 19331798

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  • 亞細(xì)胞定位：
    Nucleus, nucleoplasm. Cytoplasm.
  • 組織特異性：
    Ubiquitous.
  • 數(shù)據(jù)庫(kù)鏈接：