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  4. Mouse Collagen Type Ⅳ,Col Ⅳ ELISA KIT

Mouse Collagen Type Ⅳ,Col Ⅳ ELISA KIT

  • 中文名稱：
    小鼠Ⅳ型膠原(Col Ⅳ)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08884m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠Ⅳ型膠原(Col Ⅳ)酶聯(lián)免疫試劑盒（CSB-E08884m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Col Ⅳ含量。Col Ⅳ，即IV型膠原，是一種主要的細(xì)胞外基質(zhì)蛋白，在多種生理過程中扮演關(guān)鍵角色，如血管生成、組織修復(fù)和細(xì)胞粘附。其研究機(jī)制涉及與MMP-12等酶的相互作用，以及通過降解Col-Ⅳ來誘導(dǎo)血管生成等生物學(xué)過程。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL，適用于科研領(lǐng)域中小鼠模型的相關(guān)研究，例如通過檢測血清/血漿中Col Ⅳ濃度評估全身性基底膜代謝狀態(tài)，或通過分析肝、腎等組織勻漿的Col Ⅳ表達(dá)水平，探究器官特異性纖維化進(jìn)展及藥物干預(yù)效果?？蓾M足體外實(shí)驗(yàn)中對Col Ⅳ動態(tài)變化的定量分析需求，為基底膜相關(guān)機(jī)制研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Collagen Type 4,Col 4,_x000D_ Collagen Type IV,Col IV
  • 縮寫：
    Col Ⅳ
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.78 ng/mL-50 ng/mL
  • 靈敏度：
    0.195 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse COL Ⅳ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 100  
    Range % 97-103  
    1:2 Average % 89  
    Range % 84-93  
    1:4 Average % 88  
    Range % 84-92  
    1:8 Average % 101  
    Range % 97-106  
  • 回收率：
    The recovery of mouse COL Ⅳ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 85 81-90  
    EDTA plasma (n=4) 96 93-99  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    50 2.457 2.569 2.513 2.334  
    25 1.612 1.608 1.610 1.431  
    12.5 0.949 0.968 0.959 0.780  
    6.25 0.537 0.548 0.543 0.364  
    3.12 0.394 0.375 0.385 0.206  
    1.56 0.304 0.312 0.308 0.129  
    0.78 0.259 0.262 0.261 0.082  
    0 0.178 0.179 0.179    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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