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  4. Mouse Corticotropin-releasing factor-binding protein(CRHBP) ELISA kit

Mouse Corticotropin-releasing factor-binding protein(CRHBP) ELISA kit

  • 中文名稱：
    小鼠促腎上腺皮質(zhì)激素釋放因子結(jié)合蛋白(CRHBP)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL005964MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠促腎上腺皮質(zhì)激素釋放因子結(jié)合蛋白(CRHBP)酶聯(lián)免疫試劑盒（CSB-EL005964MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的CRHBP含量。CRHBP即促腎上腺皮質(zhì)激素釋放激素結(jié)合蛋白，它在應(yīng)激反應(yīng)調(diào)節(jié)等方面有重要作用。其研究機(jī)制主要圍繞它與促腎上腺皮質(zhì)激素釋放激素（CRH）的結(jié)合展開，通過(guò)結(jié)合CRH影響其生物活性，進(jìn)而調(diào)控下丘腦-垂體-腎上腺軸（HPA軸）的功能。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，參與應(yīng)激反應(yīng)、能量代謝及神經(jīng)內(nèi)分泌功能的動(dòng)態(tài)平衡研究；探究應(yīng)激相關(guān)疾病模型中CRHBP的動(dòng)態(tài)變化、評(píng)估HPA軸功能異常相關(guān)機(jī)制，或用于神經(jīng)內(nèi)分泌調(diào)控的基礎(chǔ)研究；助力于神經(jīng)生物學(xué)、內(nèi)分泌學(xué)及行為醫(yī)學(xué)等領(lǐng)域的實(shí)驗(yàn)研究本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    CrhbpCorticotropin-releasing factor-binding protein ELISA kit; CRF-BP ELISA kit; CRF-binding protein ELISA kit; Corticotropin-releasing hormone-binding protein ELISA kit; CRH-BP ELISA kit
  • 縮寫：
    CRHBP
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CRHBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 89-97  
    1:2 Average % 96  
    Range % 91-103  
    1:4 Average % 97  
    Range % 94-100  
    1:8 Average % 93  
    Range % 88-98  
  • 回收率：
    The recovery of mouse CRHBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 93-100  
    EDTA plasma (n=4) 91 84-98  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.582 2.776 2.679 2.501  
    500 2.193 2.223 2.208 2.030  
    250 1.446 1.465 1.456 1.278  
    125 0.821 0.865 0.843 0.665  
    62.5 0.565 0.612 0.589 0.411  
    31.25 0.319 0.295 0.307 0.129  
    15.6 0.256 0.246 0.251 0.073  
    0 0.178 0.177 0.178 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Binds CRF and inactivates it. May prevent inappropriate pituitary-adrenal stimulation in pregnancy.
  • 基因功能參考文獻(xiàn)：
    1. Repeated cycles of binge drinking alter CRF-BP mRNA expression in the medial prefrontal cortex. PMID: 27374820
    2. Ventral tegmental area CRH-BP is involved in the initial stages of escalated ethanol drinking. PMID: 26247973
    3. These studies demonstrate a role for the pituitary CRH-BP in attenuating the HPA response to stress in female mice. PMID: 25731977
    4. estrogen-regulated expression of corticotropin-releasing hormone-binding protein in lactotropes in female mice suggests that the pituitary is an important site for interactions between the hypothalamic-pituitary-adrenal axis and other endocrine systems PMID: 12446601
    5. Evidence is presented suggesting that the CRH-BP may be required for corticotropin-releasing factor-induced potentiation of NMDA receptors in dopaminergic neurons of the ventral tegmental area. PMID: 12895416
    6. GnRH induces a 3.7-fold increase in CRH-BP mRNA levels via multiple intracellular signaling pathways and a multipartite GnRH response element. PMID: 15976007
    7. analysis of regulation and function of CRH-BP [review] PMID: 16368564
    8. Deletion of corticotropin-releasing factor binding protein selectively impairs maternal, but not intermale aggression. PMID: 18929624

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    CRF-binding protein family
  • 數(shù)據(jù)庫(kù)鏈接：