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  4. Mouse Creatine kinase M-type(CKM) ELISA kit

Mouse Creatine kinase M-type(CKM) ELISA kit

  • 中文名稱：
    小鼠M型肌酸激酶(CKM)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL005459MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠M型肌酸激酶(CKM)酶聯(lián)免疫試劑盒（CSB-EL005459MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的CKM含量。試劑盒檢測(cè)范圍為1.56 mU/mL-100 mU/mL，適用于小鼠模型研究中肌肉損傷評(píng)估、運(yùn)動(dòng)生理學(xué)實(shí)驗(yàn)或藥物干預(yù)后能量代謝變化的分析，例如肌肉疾病模型機(jī)制研究、運(yùn)動(dòng)性肌損傷評(píng)價(jià)或心肌代謝相關(guān)藥物的科研篩選，為肌肉相關(guān)疾病的病理機(jī)制探索提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Ckm ELISA Kit; Ckmm ELISA Kit; Creatine kinase M-type ELISA Kit; EC 2.7.3.2 ELISA Kit; Creatine kinase M chain ELISA Kit; Creatine phosphokinase M-type ELISA Kit; CPK-M ELISA Kit; M-CK ELISA Kit
  • 縮寫：
    CKM
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma
  • 檢測(cè)范圍：
    1.56 mU/mL-100 mU/mL
  • 靈敏度：
    0.39 mU/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CKM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %90
    Range %86-95
    1:2Average %98
    Range %92-100
    1:4Average %90
    Range %87-96
    1:8Average %91
    Range %85-95
  • 回收率：
    The recovery of mouse CKM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-99
    EDTA plasma (n=4)9995-106
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/mlOD1OD2AverageCorrected
    1002.893 3.112 3.003 2.890
    502.323 2.394 2.359 2.246
    251.487 1.555 1.521 1.408
    12.50.902 0.980 0.941 0.828
    6.250.431 0.408 0.420 0.307
    3.120.347 0.321 0.334 0.221
    1.560.248 0.260 0.254 0.141
    00.115 0.110 0.113
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • 基因功能參考文獻(xiàn)：
    1. We conclude that ILK negatively and independently of PI3K regulated MEF2C phosphorylation activity and MCK mRNA expression in C2C12 cells PMID: 26788505
    2. The Fxn KO/Mck mice tested from one to two months of age showed abnormal gait patterns accompanied by a loss in motor skills. PMID: 25765157
    3. PI3K and AKT regulated skeletal muscle differentiation by regulating the expression of Myogenin and MCK. PMID: 23732671
    4. CK-M overexpression led to significantly increased contractile function at baseline and during adrenergic stimulation and increased survival after thoracic aortic constriction-induced heart failure. PMID: 22201686
    5. strong down-regulation of MCK activity contributes to F-actin instability and induces post-translational modification of alphaB-crystallin and desmin PMID: 21768101
    6. These studies indicate that the age-associated oxidative and nitrative modification of CKm results in a decrease in its activity and may cause structural changes that promote oligomerization and aggregation. PMID: 20195383
    7. Not involved in levels of creatine nnd creatine kinase in skeletal muscle of knockout mice. PMID: 12640020
    8. one of the consequences of M-CK and AK1 deficiency is hampered phosphoryl delivery to the actomyosin ATPase, resulting in a loss of contractile performance. PMID: 12730234
    9. Allosteric activation of phosphorylase b accounts for the accelerated glycogenolysis in CK-/- muscle during contraction. PMID: 12963789
    10. Data show that Six4 is transcriptional regulatory element X (TrexBF) in skeletal myocytes and transactivates the muscle creatine kinase enhancer PMID: 14966291
    11. the expression of muscle creatine kinase (MCK) gene in the heart and skeletal muscle of mice during aging PMID: 15040454
    12. metabolic failure induced by CK deficiency profoundly affects the ability of mice to engage in chronic bouts of endurance running exercise and that this decrease in performance is also associated with muscle wasting PMID: 15831533
    13. CK-MM autoantibodies can modulate the rate of CK clearance from the circulation in myositis PMID: 16810680
    14. Data show that spatial relations among organelles of muscle cells undergo adaptation in response to nonstructural stimuli such as metabolic (creatine kinase)deficiency. PMID: 16855221
    15. The creatine kinase energy transport system was evaluated in the failing mouse heart. PMID: 17481652
    16. Dec proteins repressed a MyoD-activated promoter activity of muscle creatine kinase gene through class A E-box in an HDAC1-independent manner. PMID: 17487425
    17. Creatine kinase (CK) and adenylate kinase (AK) are especially important in facilitating energy metabolism during very high energy demands. PMID: 19156695

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  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    ATP:guanido phosphotransferase family
  • 數(shù)據(jù)庫鏈接：

    KEGG: mmu:12715

    STRING: 10090.ENSMUSP00000003643

    UniGene: Mm.2375