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  4. Mouse Cytochrome-C,Cyt-C ELISA Kit

Mouse Cytochrome-C,Cyt-C ELISA Kit

  • 中文名稱：
    小鼠細胞色素C(Cyt-C)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08532m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠細胞色素C(Cyt-C)酶聯(lián)免疫試劑盒（CSB-E08532m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的CYCS含量。CYCS即細胞色素 c，是線粒體呼吸鏈的重要組成部分。它在細胞呼吸和能量代謝中起關(guān)鍵作用。研究發(fā)現(xiàn)，CYCS還參與細胞凋亡機制，當細胞受到凋亡信號刺激時，它會從線粒體釋放到胞質(zhì)中，激活凋亡相關(guān)酶，引發(fā)細胞程序性死亡。試劑盒檢測范圍為34.3 pg/mL-2200 pg/mL，適用于科研場景下細胞凋亡機制及線粒體功能相關(guān)研究；可為神經(jīng)退行性疾病模型、心肌缺血再灌注損傷、腫瘤治療響應(yīng)等基礎(chǔ)研究提供關(guān)鍵數(shù)據(jù)；適用于實驗室規(guī)模的高通量篩選或小樣本量研究，尤其在藥物干預(yù)后線粒體功能評估、基因編輯模型驗證等場景中具有重要應(yīng)用價值。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Cycs ELISA Kit; Cytochrome c ELISA Kit; somatic ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    34.3 pg/mL-2200 pg/mL
  • 靈敏度：
    8.5 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Cyt-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 92
    Range % 88-97
    1:2 Average % 84
    Range % 80-88
    1:4 Average % 90
    Range % 85-94
    1:8 Average % 97
    Range % 92-101
  • 回收率：
    The recovery of mouse Cyt-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 101 95-105
    EDTA plasma (n=4) 87 83-91
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2200 2.352 2.512 2.432 2.240
    1100 1.615 1.578 1.597 1.405
    550 1.073 1.023 1.048 0.856
    275 0.639 0.681 0.660 0.468
    137.5 0.461 0.489 0.475 0.283
    68.75 0.387 0.374 0.381 0.189
    34.3 0.318 0.305 0.312 0.120
    0 0.194 0.189 0.192 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.; Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
  • 基因功能參考文獻：
    1. the amount of cytochrome c in lymphomyeloid cells does not affect their development and normal functioning PMID: 25716737
    2. Data suggest that peroxidase activation of cytochrome c may induce apoptosis and contribute to anti-cancer properties of alpha-tocopherol succinate. PMID: 25278024
    3. Data indicate that mitochondrial alpha 7 nicotinic acetylcholine receptors (alpha7 nAChRs) regulate cytochrome c (cyt c) release by engaging intramitochondrial protein kinases. PMID: 24412630
    4. proposed that mutation of residue 41, and interaction with cardiolipin, increase peroxidase activity by altering the 40-57 Omega loop and its hydrogen bond network with the propionate of haem ring A; these changes enhance access of hydrogen peroxide and substrate to the haem PMID: 24329121
    5. Data indicate that Y48H missense mutation of cytochrome c (CYCS) gene is associated with respiratory reduction and increased apoptosis in fibroblast. PMID: 24326104
    6. Translocation of a Bak C-terminus mutant from cytosol to mitochondria to mediate cytochrome C release: implications for Bak and Bax apoptotic function. PMID: 22442658
    7. mitochondrial import and direct electron transfer from cytochrome c to Rac1 modulates mitochondrial H(2)O(2) production in alveolar macrophages pulmonary fibrosis. PMID: 22157762
    8. The course of etoposide-induced apoptosis from damage to DNA and p53 activation to mitochondrial release of cytochrome c. PMID: 11864976
    9. results suggest that activation of mitochondrial ATP-dependent potassium channels protects neurons against apoptosis by preventing cytochrome c release from the mitochondria PMID: 11919514
    10. Both neurons and cancer cells strictly inhibit cytochrome c-mediated apoptosis by a mechanism dependent on glucose metabolism. PMID: 19029908

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  • 亞細胞定位：
    Mitochondrion intermembrane space. Note=Loosely associated with the inner membrane.
  • 蛋白家族：
    Cytochrome c family
  • 組織特異性：
    Found in embryos and in adult liver and heart.
  • 數(shù)據(jù)庫鏈接：