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Mouse Decorin(DCN) ELISA kit

  • 中文名稱:
    小鼠核心蛋白聚糖(DCN)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-EL006554MO
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    小鼠核心蛋白聚糖(DCN)酶聯(lián)免疫試劑盒(CSB-EL006554MO)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的DCN含量。DCN為一種靶點。它的背景可能與特定疾病的病理過程相關。研究機制方面,可能是其通過與相關分子相互作用,參與細胞信號傳導等生理過程,進而影響疾病發(fā)展,未來或許可基于此開展疾病的診斷、治療等方面的研究。試劑盒檢測范圍為78 pg/mL-5000 pg/mL,靈敏度為19.5 pg/mL。組織修復、纖維化疾病及腫瘤微環(huán)境研究;心血管疾病模型、皮膚組織再生、肝肺纖維化機制研究以及細胞外基質(zhì)動態(tài)變化分析;探索DCN在病理生理過程中的調(diào)控作用本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    Dcn ELISA Kit; Decorin ELISA Kit; Bone proteoglycan II ELISA Kit; PG-S2 ELISA Kit; PG40 ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    78 pg/mL-5000 pg/mL
  • 靈敏度:
    19.5 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse DCN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    90

    Range %

    83-94

    1:2

    Average %

    107

    Range %

    97-112

    1:4

    Average %

    94

    Range %

    86-98

    1:8

    Average %

    88

    Range %

    81-92

  • 回收率:

    The recovery of mouse DCN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    94

    88-100

    EDTA plasma (n=4)

    88

    81-93

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    5000

    2.005

    1.911

    1.958

    1.808

    2500

    1.192

    1.142

    1.167

    1.017

    1250

    0.809

    0.789

    0.799

    0.649

    625

    0.490

    0.503

    0.497

    0.347

    312

    0.357

    0.366

    0.362

    0.212

    156

    0.280

    0.295

    0.288

    0.138

    78

    0.205

    0.214

    0.210

    0.060

    0

    0.148

    0.152

    0.150

     

  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能:
    May affect the rate of fibrils formation.
  • 基因功能參考文獻:
    1. The absence of a dermatan sulfate chain on decorin does not appear to overtly influence its functional properties in vivo. PMID: 28412940
    2. Fibroblasts differentiated to adipocytes and treated with TIP39 also showed increased decorin and production of chondroitin sulfate. Furthermore, the skin of PTH2R(-/-) mice showed abnormal extracellular matrix structure, decreased decorin expression, and skin hardness. PMID: 28454729
    3. The disruption of decorin-restricted TGFbeta signalling leads to higher stiffness of articular cartilage matrix, rendering joints more resistant to osteoarthritis. PMID: 27377816
    4. We could show that ablation of either candidate enhanced adipogenesis in visceral preadipocytes, while treatment of primary cultures with recombinant Sparcl1 and Dcn blocked adipogenesis in a dose dependent manner. In conclusion, our data suggests that the differences in adipogenesis between depots might be due to paracrine and autocrine feedback mechanisms which could in turn contribute to metabolic homeostasis PMID: 27317982
    5. A synthetic peptide corresponding to this decorin region dose-dependently inhibited the response to myostatin in cardiomyocytes PMID: 27559042
    6. Systemic delivery of an oncolytic adenovirus expressing decorin for the treatment of breast cancer bone metastases reduced tumor burden and inhibited bone destruction. PMID: 26467629
    7. Decorin is an autophagy-inducible proteoglycan and is required for proper in vivo autophagy. PMID: 26344480
    8. The results suggest that decorin plays a dual role in AAA. Adventitial decorin in normal aorta may protect against the development of AAA PMID: 25781946
    9. Development of congenital stromal dystrophy is dependent on export and extracellular deposition of truncated decorin. PMID: 26029887
    10. decorin may modulate follicular cycling and morphogenesis PMID: 24816226
    11. We found that decorin is abundantly secreted and deposited in normal connective tissue but its expression is consistently decreased in the tumor microenvironment. PMID: 24634138
    12. Decorin signaling supported fetal membrane remodeling at early stages of gestation in a TGFbeta-dependent manner, and fetal membrane stabilization at later stages of gestation without changes in TGFbeta levels. PMID: 24373743
    13. A decorin-deficient matrix affects skin chondroitin/dermatan sulfate levels and keratinocyte function. PMID: 24447999
    14. Decorin deficiency promotes hepatic carcinogenesis. PMID: 24361483
    15. decorin secreted from myotubes in response to exercise is involved in the regulation of muscle hypertrophy and hence could play a role in exercise-related restructuring processes of skeletal muscle. PMID: 24996176
    16. the gene encoding the small leucine-rich proteoglycan decorin is repressed by FOXD1 in cortical interstitial cells, compound genetic inactivation of DCN partially rescues the failure of progenitor cell differentiation in the Foxd1 null. PMID: 24284212
    17. Dimerization could be abolished by engineering glycosylation sites into the dimer interface; other interface mutants remained dimeric. The monomeric mutants were as stable as wild-type decorin in thermal unfolding experiments. PMID: 24169694
    18. Decorin lacking c-terminal repeat is retained intracellularly, its accumulation triggering endoplasmic reticulum stress that results in abnormal synthesis and secretion, leading to congenital stromal corneal dystrophy. PMID: 23685109
    19. Alterations in glycosylated decorin core protein might be implicated in myocardial remodeling and reverse remodeling, with a potential important role for CS/DS GAG chain-synthesizing enzymes. PMID: 23412898
    20. decorin induced VEGFR2-dependent mitochondrial fragmentation and loss of mitochondrial membrane potential PMID: 23798385
    21. decorin modulates delayed-type hypersensitivity responses by augmenting the induction of downstream effector cytokines of IFN-gamma and TNF-alpha, thereby influencing the recruitment of CD8(+) lymphocytes into the inflamed tissue. PMID: 23460644
    22. Lack of decorin leads to enhanced tumor formation in the liver. PMID: 23448253
    23. These data show for the first time that decorin has an impact on the biology of alpha2beta1 integrin and the vimentin intermediate filament system. PMID: 23226541
    24. DCN gene can inhibit the growth of nude mice xenograft, which is probably related with the decreased expressions of TGF-beta1 and MMP-9 protein and the inhibition of tumor angiogenesis. PMID: 23046927
    25. Decorin protein core affects the global gene expression profile of the tumor microenvironment in a triple-negative orthotopic breast carcinoma xenograft model. PMID: 23029096
    26. decorin plays a role in tendon viscoelasticity that cannot be completely explained by its role in collagen fibrillogenesis PMID: 22482685
    27. The expression of decorin, a naturally occurring TGF-beta suppressor, was elevated in Mstn-null mice. PMID: 22277753
    28. Decorin regions LRR6 and LRR5 participate in the interaction with LRP-1 and TGF-beta as well as in its dependent signaling. PMID: 22203668
    29. Wild type N-ras appears to mediate its anti-malignant effect by downregulating decorin expression. PMID: 21809347
    30. decorin prevented translational repression of PDCD4 by decreasing the activity of transforming growth factor-beta1 and the abundance of oncogenic miR-21, a translational inhibitor of PDCD4. PMID: 22087031
    31. Cleavage product of decorin serves as a functional receptor of resistin in adipocyte progenitors and may regulate white adipose tissue expansion. PMID: 21683670
    32. gradual increase in muscle regeneration PMID: 21416223
    33. these results implicated a role for decorin in mediating delayed-type hypersensitivity responses by influencing polymorphonuclear leukocyte attachment to the endothelium. PMID: 22043007
    34. Decorin is expressed in placenta/fetal membranes and is developmentally regulated in fetal membranes; data from mutant mouse strains suggest that both decorin and biglycan contribute to gestational success (i.e., prevent premature birth). PMID: 21502335
    35. We show a so far unknown function of decorin and chondroitin-6-sulfate: their ability to inhibit B16V cell migration by intracellular acidification. PMID: 21792923
    36. CTGF specifically induced the synthesis of decorin, suggesting a mechanism of autoregulation. PMID: 21454550
    37. Decorin can modify collagen I-stimulated, but not fibronectin-stimulated myoblast migration in vitro. PMID: 21059388
    38. decorin is a protective agent during the development of diabetic nephropathy PMID: 20083846
    39. Dcn has been identified as an imprinted gene by high-throughput screening using RIKEN cDNA microarray. PMID: 11820791
    40. decorin is dramatically induced in the desmin-null myocardium PMID: 11891192
    41. decorin exerts beneficial effects on tubulointerstitial fibrosis PMID: 11891213
    42. decorin binds fibrinogen in a Zn2+-dependent interaction PMID: 12582160
    43. decorin and biglycan play distinct roles in palatogenesis, and decorin was more actively involved in the process of secondary palate formation than biglycan PMID: 12666199
    44. Inhibition of myoblast migration via decorin expression is critical for normal skeletal muscle differentiation. PMID: 12871697
    45. These alterations in lung tissue mechanical behavior in Dcn-/- mice support a critical role for decorin in the formation of the lung collagen network. PMID: 15447936
    46. in DCN-deficient mice, the growth of corneal vessels was significantly diminished PMID: 15528932
    47. Results describe the effect of bone morphogenetic protein-2 (BMP-2) on the synthesis of proteoglycan during osteogenic conversion of myoblasts and suggest a role for decorin in cell response to BMP-2. PMID: 15920756
    48. decorin is not required for cell survival PMID: 15949467
    49. decorin is required for the proper fibrotic evolution of myocardial infarctions PMID: 15949932
    50. systemic overexpression of decorin reduces inflammation, triglycerides and fibrosis in atherosclerotic plaques of ApoE(-/-) mice resulting in slowing down of disease progression PMID: 16183063

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  • 亞細胞定位:
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族:
    Small leucine-rich proteoglycan (SLRP) family, SLRP class I subfamily
  • 數(shù)據(jù)庫鏈接: