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  4. Mouse Disintegrin and metalloproteinase domain-containing protein 33(ADAM33) ELISA kit

Mouse Disintegrin and metalloproteinase domain-containing protein 33(ADAM33) ELISA kit

  • 中文名稱：
    小鼠解整合素樣金屬蛋白酶33(ADAM33)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL001290MO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠解整合素樣金屬蛋白酶33(ADAM33)酶聯(lián)免疫試劑盒（CSB-EL001290MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ADAM33含量。ADAM33是一種編碼解整合素和金屬蛋白酶的基因，其表達與哮喘、慢性阻塞性肺疾病和鼻炎等疾病相關。該基因通過參與細胞粘附、遷移和信號傳導過程，在多種生物過程中發(fā)揮作用，如受精、肌肉發(fā)育和神經(jīng)發(fā)生。ADAM33基因的多態(tài)性與哮喘等疾病的易感性有關。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于基礎研究中ADAM33的表達水平分析，如探索肺部疾病模型中的分子調控機制、評估藥物干預對氣道重塑的影響，或驗證基因編輯動物模型中靶蛋白的表達變化。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
  • 別名：
    Adam33Disintegrin and metalloproteinase domain-containing protein 33 ELISA kit; ADAM 33 ELISA kit; EC 3.4.24.- ELISA kit
  • 縮寫：
    ADAM33
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADAM33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %93
    Range %88-97
    1:2Average %84
    Range %80-88
    1:4Average %95
    Range %90-100
    1:8Average %104
    Range %99-109
  • 回收率：
    The recovery of mouse ADAM33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9085-94
    EDTA plasma (n=4)9287-96
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.658 2.552 2.605 2.434
    5001.881 2.006 1.944 1.773
    2501.128 1.204 1.166 0.995
    1250.627 0.653 0.640 0.469
    62.50.372 0.401 0.387 0.216
    31.20.276 0.264 0.270 0.099
    15.60.234 0.239 0.237 0.066
    00.174 0.167 0.171
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 基因功能參考文獻：
    1. The mouse and human predicted proteins consisted of 797 and 813 amino acids, respectively, and they shared 70% homology of the entire amino acid sequence. PMID: 11814695
    2. Identification and preliminary characterization of mouse Adam33. PMID: 11897009
    3. ADAM33 is not essential for growth or reproduction in the mouse and does not modulate baseline or allergen-induced airway responsiveness. PMID: 16943435
    4. Adam33/Adam33 shows 2 significant increments in expression during lung morphogenesis, suggesting important developmental regulation. PMID: 19665773
  • 亞細胞定位：
    Membrane; Single-pass type I membrane protein.
  • 數(shù)據(jù)庫鏈接：