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  4. Mouse Ephrin-A1(EFNA1) ELISA kit

Mouse Ephrin-A1(EFNA1) ELISA kit

  • 中文名稱：
    小鼠肝配蛋白A1受體(EFNA1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL007460MO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠肝配蛋白A1受體(EFNA1)酶聯(lián)免疫試劑盒（CSB-EL007460MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的EFNA1含量。EFNA1 即艾弗林 A1，是 Eph 受體酪氨酸激酶的膜結(jié)合配體。在腫瘤等疾病中，EFNA1 與其受體結(jié)合，參與調(diào)節(jié)細胞黏附、遷移、增殖等。研究聚焦其在腫瘤血管生成、侵襲轉(zhuǎn)移機制，有望為相關(guān)疾病治療提供新靶點和策略。試劑盒檢測范圍為93.75 pg/mL-6000 pg/mL，適用于基礎(chǔ)科研領(lǐng)域，如探究肝損傷模型、腫瘤微環(huán)境或再生醫(yī)學(xué)研究中EFNA1的表達調(diào)控機制，亦可應(yīng)用于藥物干預(yù)實驗或信號通路研究中靶點蛋白的動態(tài)監(jiān)測，為分子機制研究提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Efna1 ELISA Kit; Epgl1 ELISA Kit; Epl1 ELISA Kit; Lerk1 ELISA Kit; Ephrin-A1 ELISA Kit; EPH-related receptor tyrosine kinase ligand 1 ELISA Kit; LERK-1 ELISA Kit; Immediate early response protein B61) [Cleaved into: Ephrin-A1 ELISA Kit; secreted form] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    93.75 pg/mL-6000 pg/mL
  • 靈敏度：
    23.43 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse EFNA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 84
    Range % 80-88
    1:2 Average % 96
    Range % 93-99
    1:4 Average % 87
    Range % 83-90
    1:8 Average % 105
    Range % 100-110
  • 回收率：
    The recovery of mouse EFNA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 87 80-94
    EDTA plasma (n=4) 102 98-106
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    6000 2.702 2.694 2.698 2.530
    3000 2.184 2.201 2.193 2.025
    1500 1.585 1.602 1.594 1.426
    750 1.088 1.069 1.079 0.911
    375 0.669 0.679 0.674 0.506
    187.5 0.453 0.468 0.461 0.293
    93.75 0.314 0.330 0.322 0.154
    0 0.168 0.167 0.168 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis.
  • 基因功能參考文獻：
    1. EphA2 mAb treatment could partially inhibit LPSinduced inactivation of EphBEphrin B3 signalling, while Ephrin B3 overexpression could abrogate LPSinduced activation of EphA2Ephrin A1 signalling. EphB1/Ephrin B3 signalling may antagonise the EphA2/Ephrin A1dependent pathway following LPS treatment. PMID: 29901151
    2. Collectively, these data suggest that ATRA attenuates bleomycin-induced pulmonary fibrosis by regulating EphA2-EphrinA1 and PI3K-Akt signaling. PMID: 28743499
    3. Lipopolysaccharide exposure significantly up-regulated EphA2 and EphrinA1 expression. PMID: 27549114
    4. Activation of EphA1-Epha receptor axis attenuates diabetic nephropathy in mice. PMID: 28341121
    5. Following ephrin-A1 stimulation, truncated EphA2 did not detectably interfere with the phosphorylation of endogenous EphA2, and it potentiated cell adhesion possibly through modulation of integrin avidity. PMID: 27665280
    6. ADAM12 enhanced ephrin-A1 cleavage in response to transforming growth factor-betra1 in primary tumors. PMID: 23686306
    7. S100A8 and ephrin-A1 contribute to lung metastasis. PMID: 24103748
    8. We used in utero electroporation-mediated EphA7 overexpression in developing somatosensory corticothalamic axons to dissect EphA7/ephrin-A-dependent mechanisms involved in regulating both initial targeting and postnatal growth of the CT projections. PMID: 22821544
    9. Elevated levels of ephrin-A1 may contribute to diabetic keratopathies by persistently engaging EphA2 and prohibiting Akt-dependent corneal epithelial repair processes. PMID: 22247486
    10. Data indicate that ephrin-A1 regulates cardiac valve development, making ephrin-A1-deficient mice a novel model for congenital heart defects. PMID: 20960543
    11. Cooperation between Slit2 and ephrin-A1 regulates a balance between the pro- and antiangiogenic functions of Slit2. PMID: 21135133
    12. ephrin-A1 has a positive role in tumor growth in vivo, and are consistent with previous reports of ephrin-A1 acting through EphA receptors in the tumor microenvironment in vivo. PMID: 20154726
    13. When NIH3T3 cells were plated onto an ephrinA1-coated surface, the cells both adhered and spread. PMID: 12134157
    14. Immunohistological analyses reveal strong ephrin-A1 expression in lung tissue, low expression in cortical areas of lymph nodes, and none in T cell/B cell areas of the spleen. PMID: 14707054
    15. Whole-mount in situ hybridization revealed overlapping expression of the Epha1 receptor and its high-affinity ligands ephrin A1 (Efna1) and ephrin A3 (Efna3) in the primitive streak and the posterior paraxial mesoderm during early mouse development. PMID: 16466970
    16. Ephrins A1 and A5 are substrates for a cross-linking enzyme, tissue transglutaminase, which mediates the formation of oligomeric ephrin. PMID: 17707797
    17. Increased expression of ephrin-A1 accelerated the malignant progression of the intestinal adenoma to invasive tumors. PMID: 18246128
    18. HIF-2alpha plays an essential role in vascular remodeling during tumor vascularization through activation of at least ephrin A1. PMID: 18434321
    19. Radiotherapy-induced changes in ephrin-A1 gene expression related with angiogenesis may modulate microenvironment and influence responsiveness of tumors. PMID: 18997097

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  • 亞細胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor.; [Ephrin-A1, secreted form]: Secreted.
  • 蛋白家族：
    Ephrin family
  • 組織特異性：
    Expressed in myogenic progenitor cells.
  • 數(shù)據(jù)庫鏈接：