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  4. Mouse Epidermal growth factor,EGF ELISA Kit

Mouse Epidermal growth factor,EGF ELISA Kit

  • 中文名稱：
    小鼠表皮生長因子(EGF)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08028m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠表皮生長因子(EGF)酶聯(lián)免疫試劑盒（CSB-E08028m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的EGF含量。EGF即表皮生長因子，廣泛存在于人體組織和體液中。它能與細(xì)胞膜上的受體結(jié)合，激活下游信號通路，調(diào)控細(xì)胞生長、增殖和分化。在醫(yī)學(xué)研究中，其信號通路異常與多種疾病相關(guān)，是藥物研發(fā)等領(lǐng)域的重要靶點(diǎn)。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，靈敏度為1.95 pg/mL。主要應(yīng)用于基礎(chǔ)科研場景，包括但不限于小鼠疾病模型構(gòu)建、藥物干預(yù)效果評估、細(xì)胞培養(yǎng)上清液分析等實(shí)驗(yàn)體系，為探索EGF在生理調(diào)控或病理狀態(tài)下的動態(tài)變化提供可靠工具，實(shí)驗(yàn)數(shù)據(jù)需結(jié)合其他分子生物學(xué)方法進(jìn)行系統(tǒng)驗(yàn)證。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    EgfPro-epidermal growth factor ELISA Kit; EGF) [Cleaved into: Epidermal growth factor] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    7.8 pg/mL-500 pg/mL
  • 靈敏度：
    1.95 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:2

    Average %

    89

    Range %

    80-92

    1:4

    Average %

    93

    Range %

    91-105

    1:8

    Average %

    95

    Range %

    90-103

    1:16

    Average %

    93

    Range %

    86-98

  • 回收率：

    The recovery of mouse EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    89-98

    EDTA plasma (n=4)

    93

    90-100

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    500

    2.638

    2.684

    2.661

    2.503

    250

    2.154

    2.067

    2.111

    1.953

    125

    1.671

    1.638

    1.655

    1.497

    62.5

    1.126

    1.079

    1.103

    0.945

    31.2

    0.693

    0.711

    0.702

    0.544

    15.6

    0.382

    0.363

    0.373

    0.215

    7.8

    0.295

    0.284

    0.290

    0.132

    0

    0.161

    0.155

    0.158

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    EGF（表皮生長因子）是一種生長因子蛋白，參與細(xì)胞增殖、分化和存活等生理過程。最新研究表明，EGF可作為一種重要的癌癥治療靶點(diǎn)，對多種惡性腫瘤的治療具有潛在的作用。此外，EGF還可以促進(jìn)細(xì)胞的再生和修復(fù)，因此在皮膚科學(xué)和組織修復(fù)領(lǐng)域也有廣泛的應(yīng)用。最近的一項(xiàng)研究發(fā)現(xiàn)，EGF在視網(wǎng)膜病變和神經(jīng)系統(tǒng)恢復(fù)中也具有重要作用，為這些疾病的治療提供了新的思路和策略。
  • 功能：
    EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6.
  • 基因功能參考文獻(xiàn)：
    1. In mice, both EGF and pimecrolimus groups showed less erythema with significantly reduced inflammation and decreased expression of thymic stromal lymphopoietin. EGF relieved S. aureus-induced inflammation and AD-like skin lesions in Nc/Nga mice. PMID: 29862299
    2. hijacks miR-198/FSTL1 wound-healing switch and steers a two-pronged pathway toward metastasis PMID: 28827448
    3. These results indicate that Kindlin-1 is essential in EGF-induced re-epithelialization in skin wound healing and provide additional rationale for the clinical application of EGF in the treatment of acute wounds. PMID: 28290610
    4. concentration of EGF is critical for the switch between hair follicle growth and inhibition, and EGF promotes DP cell proliferation via Notch signaling pathway PMID: 27109378
    5. EGF promotes FoxM1 expression through the ERK signal pathway PMID: 26022336
    6. Data indicate that Sonic hedgehog (Shh) stimulate branching morphogenesis (BrM) and induced synthesis of mRNAs for Ptch1 protein, epidermal growth factor (EGF) and receptors of the ErbB receptors ErbB1, ErbB2 and ErbB3. PMID: 26930157
    7. Either LIF or EGF is needed during development of pre-implantation embryo. PMID: 27096934
    8. PXR activation stimulates EGF-mediated hepatocyte proliferation in mice, at least in part, through inhibiting FOXO3 from accelerating cell-cycle progression. PMID: 26574435
    9. Data (including data from studies in knockout mice) suggest that Epab (embryonic poly(A)-binding protein), which is oocyte specific, is required for ability of cumulus cells and granulosa cells to exhibit responsiveness to Egf/Egfr signaling. PMID: 26492470
    10. modulation of EGF signaling affects in vitro expansion and differentiation of progenitors from embryonic pancreas of both mice and man. PMID: 25925840
    11. TLR4 blockade prevented TPN-associated intestinal mucosa atrophy by preserving proliferation and preventing apoptosis. This is driven by a reduction in TNF-alpha abundance and increased EGF. PMID: 25782989
    12. EGF is required for cardiac differentiation of P19CL6 cells through interaction with GATA-4 in a time- and dose-dependent manner. PMID: 25504289
    13. These data demonstrate that Mcl-1 is essential for mammopoiesis and identify EGF as a critical trigger of Mcl-1 translation to ensure survival of milk-producing alveolar cells. PMID: 25730472
    14. results indentify EGF signalling as a robust vasculogenic inductive pathway for ATMCs, leading to their transdifferentiation into functional VSMC-like cells. PMID: 24967966
    15. MEKK1 PHD controls p38 and JNK activation during TGF-beta, EGF and microtubule disruption signalling, but does not affect MAPK responses to hyperosmotic stress. PMID: 25260751
    16. VEGFR1-mediated signaling plays a critical role in gastric ulcer healing and angiogenesis through enhanced EGF expression on VEGFR1+CXCR4+ cells PMID: 23982810
    17. The combination of EGF-FGF2 stimulates the proliferation. PMID: 24907656
    18. IL-6 may act as a new potential cumulus expansion-related transcript, which may be involved in the integration of TrkA and EGF signaling in affecting expansion of cumulus oocyte complexes. PMID: 24215827
    19. EGF treatment increases Cx43 phosphorylation up to 4-fold and induces efficient gap junction endocytosis. PMID: 24492000
    20. A previously unrecognized protective role is played by EGF in atopic dermatitis. EGF also has a new role in modulating IL-17 responses in the skin. PMID: 24337738
    21. Insulin treatment resulted in increased vascular leakage apparently mediated by betacellulin and signaling via the epidermal growth factor (EGF) receptor. PMID: 23831329
    22. While EGF activated Gab1 and Shc equally, within the same concentration range, HGF very potently and almost exclusively activated Gab1, having only a minimal effect on Shc PMID: 24126105
    23. These results support the argument that aberrant hyper-signals of EGF have significant impact on mouse behavioral traits and dopamine metabolism. PMID: 23669645
    24. Data indicate that LTB4 receptor type 1 (BLT1)-knockout mice showed delayed liver repair and a 70-80% attenuation in expression of epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), and VEGF receptor 1 (VEGFR1). PMID: 23629862
    25. Data suggest Egf/Egf receptor signaling in cumulus cells (CC) downregulates Npr2 (natriuretic peptide receptor 2), decreases cGMP, elevates calcium, and induces meiotic resumption/oogenesis in cultured CC-oocyte complexes (in induced meiotic arrest). PMID: 23787120
    26. EGF and insulin/IGF prime endometrial epithelial cells to direct the mitogenic effects of estradiol. Furthermore, PTEN deficiency results in enhanced responsiveness to this combination, leading to hyperplasia of endometrial cells. PMID: 23669345
    27. Upregulation of endogenous EGF may act on top of an endocrinous cascade orchestrating the interactions between Sertoli cells and germ cells and may operate as defensive mechanism in response to testicular ischemia/reperfusion stress. PMID: 23241343
    28. study elucidated a previously unknown function of EGF in promoting hematopoietic stem cell regeneration after radiation-induced myelosuppression PMID: 23377280
    29. Suggest that FAK acts as a central coordinator of integrin and growth factor-mediated S-phase entry by regulating Cdk2 in EGF-stimulated hepatocytes. PMID: 23168795
    30. a novel link between EGF receptor stimulation, ILK-containing complexes, and activation of small Rho GTPases necessary for acquisition of front-rear polarity and forward movement PMID: 22160594
    31. The effects of EGF, insulin, and insulin-like growth factor (IGF)-1 correlate with reactive oxyen species (ROS) production in kidney cortical collecting duct cells. PMID: 23135700
    32. nucleobindin-2 regulates EGF-stimulated MAPK kinase/Erk signaling, cell proliferation, and adipocyte differentiation PMID: 22514047
    33. Neurotensin increases EGF expression in skin dendritic cells. PMID: 21767580
    34. It was suggested that, in this in vitro mouse model, EGF signaling during ovulation might protect the cumulus cells from the potential luteinizing effects of LH. PMID: 21293035
    35. Enteric glia promote intestinal mucosal healing via activation of focal adhesion kinase and release of proEGF. PMID: 21350188
    36. Rac1 through NADPH oxidase is part of the signaling pathway constituted by FAK, Rac1, and ERK that regulates focal adhesion disassembly during cell spreading. PMID: 21660950
    37. A switch in autocrine signaling to foster tumor growth that was initially triggered by EGF. PMID: 21464922
    38. The Tgf-beta(3) null mutant mouse palate presents several cellular anomalies that lead to the appearance of cleft palate reveals misexpression of EGF and Msx-1. PMID: 20881363
    39. Proprotein convertase PC7 enhances the activation of the EGF receptor pathway through processing of the EGF precursor. PMID: 21209099
    40. EGF signaling is indispensable for activation of proliferation and inhibition of unexpected cell death in intestinal epithelial cells PMID: 20714325
    41. EGF-induced MAPK signaling inhibits hemidesmosome formation through phosphorylation of the integrin beta 4. PMID: 20870721
    42. EGF/EGR-1 repressed transcriptional activation of the MMP-9 gene by stromal cells. PMID: 20472833
    43. 7,3',4'-Trihydroxyisoflavone inhibits epidermal growth factor-induced cell proliferation and transformation by suppressing cyclin-dependent kinases and phosphatidylinositol 3-kinase PMID: 20444693
    44. Phosphatidylinositol 4,5-bisphosphate activates Slo3 currents and its hydrolysis underlies the epidermal growth factor-induced current inhibition PMID: 20392696
    45. regulation of beta1-integrin expression/localization is involved in cellular processes, such as proliferation, induced by bFGF and EGF in mouse neuroepithelial cells PMID: 20371608
    46. conclude that neural stem/progenitor cell subpopulations reside in neurospheres that are distinguishable by their responsiveness to fibroblast growth factor-2 and EGF which is differentially regulated by chondroitin sulfate-carbohydrate structures. PMID: 20087964
    47. Palmitoylated EGFR exhibited only low-affinity EGF binding and impaired signal transduction. PMID: 19196031
    48. GH modulates EGF signaling in the liver. PMID: 20032199
    49. EGF is a key regulator of cell growth and development and a sevenfold increase is shown in EGF expression in FVB compared to B10.Q mice. PMID: 20080754
    50. Absence of post-translational aspartyl beta-hydroxylation of domains in mice leads to developmental defects and an increased incidence of intestinal neoplasia PMID: 11773073

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  • 亞細(xì)胞定位：
    Membrane; Single-pass type I membrane protein.
  • 數(shù)據(jù)庫鏈接：